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Influence Of The Metastatic Microenvironment On Stem-Like Human Breast Cancer Cells, Ashkan Sadri
Influence Of The Metastatic Microenvironment On Stem-Like Human Breast Cancer Cells, Ashkan Sadri
Electronic Thesis and Dissertation Repository
Ninety percent of breast cancer-related mortalities result from metastasis. We have previously demonstrated that stem-like ALDHhiCD44+ breast cancer cells are critical for metastasis, and preferentially target the lung and bone marrow (BM). We hypothesize organ tropism occurs through promotion of the ALDHhiCD44+ phenotype. Using a 2D ex vivo model, lung and BM conditioned media (CM) were utilized to assess their influence on stem-like phenotype and behavior. Exposure of human breast cancer cells to lung-CM significantly decreased the proportion of cells with a stem-like ALDHhiCD44+ phenotype, decreased expression of cancer stem cell (CSC)-related genes, and increased gene expression related to migration …