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Characterization Of Blood-Derived Human Progenitor Cells For Vascular Regeneration, David Putman
Characterization Of Blood-Derived Human Progenitor Cells For Vascular Regeneration, David Putman
Electronic Thesis and Dissertation Repository
Cardiovascular disease (CVD) remains a leading cause of premature death worldwide. Despite advances in treatment of ischemic diseases including myocardial infarction and peripheral arterial disease (PAD) there remains a need for effective revascularization therapies. Although early cell therapy trials investigated use of MNC from autologous bone marrow, emerging evidence indicates that purified progenitor cell populations are required to induce optimal vascular regeneration. In addition, autologous cells show reduced efficacy due to CVD-related progenitor dysfunction. This thesis presents preclinical studies characterizing several blood-derived cell types for the development of cellular therapies to treat PAD, focusing on the use of allogeneic umbilical …
Islet Regenerative Properties Of Ex Vivo Expanded Hematopoietic Progenitor Cells, Ayesh K. Seneviratne
Islet Regenerative Properties Of Ex Vivo Expanded Hematopoietic Progenitor Cells, Ayesh K. Seneviratne
Electronic Thesis and Dissertation Repository
Human umbilical cord blood (UCB) progenitor cells with high aldehyde dehydrogenase activity (ALDHhi), can stimulate endogenous islet regeneration after transplantation into mice with steptozotocin (STZ)-induced diabetes. However, UCB ALDHhi cell are extremely rare, and expansion will be required to develop cell-mediated strategies to treat patients with diabetes. To increase the number of progenitor cells available for clinical application, we expanded ALDHhi UCB cells under clinically applicable, serum-free hematopoietic-restricted conditions. 6 day expansion resulted in a 15-fold increase in total cell number, and a 3-fold increase in the number of HPC retaining high ALDH (ALDHhi HPC) …