Open Access. Powered by Scholars. Published by Universities.®
- Discipline
Articles 1 - 2 of 2
Full-Text Articles in Life Sciences
Connexin-32 And Connexin-43 Immunoreactivity In Rodent Taste Buds, Amanda E. Bond
Connexin-32 And Connexin-43 Immunoreactivity In Rodent Taste Buds, Amanda E. Bond
Electronic Theses and Dissertations
Studies indicate that ATP is one of the primary neurotransmitters in taste transduction. ATP release occurs from taste cells via specific hemichannels such as pannexin/connexin hemichannels (Huang et al., 2007; Romanov et al., 2007). We hypothesize that Type II (receptor) and possibly Type III (presynaptic) cells release ATP at sites containing pannexin/connexin hemichannels. In this study, we examine the presence of connexin–32–LIR (Like Immunoreactivity) and connexin–43–LIR in rodent taste buds through immunocytochemical analysis and DAB (Di–amino–benzidine) immunoelectron microscopy. We observed that connexin–32–LIR co–localizes with P2X2–LIR in nerve fibers and in a small subset of NCAM–LIR cells. Connexin–32–LIR does not co–localize …
Dopamine-Beta-Hydroxylase And Norepinephrine Transporter Immunoreactivity In Rat Taste Buds, Jenna Ashley Walton
Dopamine-Beta-Hydroxylase And Norepinephrine Transporter Immunoreactivity In Rat Taste Buds, Jenna Ashley Walton
Electronic Theses and Dissertations
Of the handful of neurotransmitters modulating taste response, norepinephrine (NE) may be the least understood. Although the physiological role of NE has been tested, it is still unclear whether the rat taste bud may serve as an endogenous source of neurotransmitter or if it must be taken up from outside the taste bud. Immunocytochemical analysis of DBH has shown that DBH–LIR is present in taste cells of the rat circumvallate papillae. Specifically, DBH–LIR is present in a subset of Type II taste cells. Nearly all (98%) DBH–LIR cells express PLCβ2–LIR, and about half (41%) of PLCβ2–LIR cells express DBH–LIR. DBH–LIR …