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Full-Text Articles in Life Sciences

Isolation And Characterization Of Bacteriophages From Fish Tank Samples, Oluwaseyi Oluwagbeminiyi Oladele-Ajose Dec 2023

Isolation And Characterization Of Bacteriophages From Fish Tank Samples, Oluwaseyi Oluwagbeminiyi Oladele-Ajose

Electronic Theses and Dissertations

Bacteriophages (phages) are viral obligate intracellular parasites that infect bacteria. Research has been carried out on alternative treatment plans for bacterial infections due to the increase in antibiotic resistance. Phages have been proposed as an alternative to antibiotics. This research focused on the isolation and characterization of bacteria from small aquariums. A total of 42 bacterial isolates were isolated from seven different fish tank water samples and were tested to determine if they possessed phages. Spot plaque assay revealed presence of lytic phage for only one of the isolated bacterial strains, which was determined to belong to Enterobacteriaceae family based …


Molecular Detection And Characterization Of Rickettsia Sp. In Hard Ticks Collected In Nacogdoches, Texas, Haley Nicole Standifird Aug 2023

Molecular Detection And Characterization Of Rickettsia Sp. In Hard Ticks Collected In Nacogdoches, Texas, Haley Nicole Standifird

Electronic Theses and Dissertations

The number of cases of tick-borne diseases is increasing in the United States. Many efforts to control ticks have been made and are continuously being developed. These methods include surveillance of ticks and detection of the tick-borne pathogens.This study examined hard ticks in Nacogdoches, Texas, to test for the presence of Rickettsia sp. 47 ticks were collected from vegetation by flagging and their species, sex, and developmental stage were determined using available keys. Individual ticks were processed for nucleic acid extraction and molecular detection of Rickettsia sp. using PCR-based methods. The citrate cynthease gene, 17kDa gene, and outer membrane protein …


Characterization Of Molecular Content, Cytotoxicity, And Anti-Inflammatory Activity Of Extractions From Eupatorium Serotinum, Jordy Sloan May 2023

Characterization Of Molecular Content, Cytotoxicity, And Anti-Inflammatory Activity Of Extractions From Eupatorium Serotinum, Jordy Sloan

Electronic Theses and Dissertations

Inflammation is a complex and varied immune response which can be expressed chronically or as a temporary reaction to foreign invaders. Symptoms of inflammation include swelling, pain, soreness, and difficulty of motion. Recently, the effect of sesquiterpene lactones such as Helenalin from Arnica montana have been studied for their anti-inflammatory properties. In this study, we identified and harvested samples of Eupatorium serotinum, a plant historically used by Native Americans to treat a variety of medical illnesses. Crude extracts of the leaves, flowers, roots, and stems were prepared by ethanol extraction. The cytotoxicity of the different extracts on HEK293 cells …


Dolphin Behavioral Responses To Uncrewed Aerial Systems As A Function Of Exposure, Height, And Type, Savannah Damiano May 2023

Dolphin Behavioral Responses To Uncrewed Aerial Systems As A Function Of Exposure, Height, And Type, Savannah Damiano

Electronic Theses and Dissertations

Uncrewed aerial systems (UAS) are becoming a standard tool in the study of cetaceans, however, a comparative assessment of animal responses to UAS has not been established to gauge the most effective systems for cetacean study. We utilized Dolphin Quest Bermuda’s eleven bottlenose dolphins as subjects for such an investigation taking place over five weeks in 2022 and five weeks in 2023. The dolphins were evaluated for investigative behavioral responses to six off-the-shelf UAS types and a custom fixed wing system. Each UAS was flown in decreasing height vertically above the main dolphin lagoon to evaluate dolphin behavioral responses in …


Detection And Occurrence Of Nerodia Harteri (Serpentes: Colubridae) In An Upper Portion Of The Brazos River Watershed, Jessica Yates May 2022

Detection And Occurrence Of Nerodia Harteri (Serpentes: Colubridae) In An Upper Portion Of The Brazos River Watershed, Jessica Yates

Electronic Theses and Dissertations

Semiaquatic snakes are disproportionately impacted by habitat alteration adjacent to wetlands because of their reliance on both terrestrial and aquatic habitats. Brazos River Watersnakes, Nerodia harteri, are endemic natricines with one of the most restricted geographic ranges in North America. I quantified detection and site occupancy probabilities of N. harteri and its sympatric congeners (N. erythrogaster and N. rhombifer), as well as microhabitat selection within the Nerodia community. Within occupied transects, search effort was positively correlated with detecting N. harteri, whereas environmental and/or habitat variables were better predictors for detecting the sympatric congeners. Microhabitat selection between …


Effect Of Mycorrhizae Inoculation On The Growth And Success Of Three Taxodium Distichum Hybrids In Saline - Impacted Coastal Soils, Elif Ilhan May 2022

Effect Of Mycorrhizae Inoculation On The Growth And Success Of Three Taxodium Distichum Hybrids In Saline - Impacted Coastal Soils, Elif Ilhan

Electronic Theses and Dissertations

In 2008, Galveston Island was severely impacted by Hurricane Ike, resulting in high salt deposition in the soil and groundwater. This caused a loss of many native plant species. A study was initiated to determine effective ways to promote the growth conditions of three bald cypress genotypes (Taxodium distichum var distichum and Taxodium distichum var mexicanum crosses) in salt-affected soils. The treatments applied were mycorrhizae inoculation, fertilizer application, and a combination of mycorrhizae inoculation and fertilizer application. A total of sixty (60) trees planted in plots of three rows and divided into five randomized replication blocks of four treatments …


Fish Community Assembly At Multiple Spatial Scales In East Texas Streams, Jake R. Swanson May 2022

Fish Community Assembly At Multiple Spatial Scales In East Texas Streams, Jake R. Swanson

Electronic Theses and Dissertations

Stream communities are structured by environmental processes that vary in strength across different spatiotemporal scales. Therefore, revealing how stream fish communities are influenced by environmental gradients may clarify how communities respond to disturbance associated with anthropogenic change. I investigated the relative role environmental variables play in the processes that structure the taxonomic and functional diversity of stream fish communities, as well as historical trends in occurrence and local habitat associated with a species of greatest conservation need within east Texas. The results of this study indicate that stream sites with more habitat complexity and stability support a more taxonomically and …


Characterization Of Hemocytes In Amblyomma Americanum And Investigation Of Traf6’S Role In Proliferation Of Hemocytes In Response To Infection, Jacquelyn T. May Apr 2022

Characterization Of Hemocytes In Amblyomma Americanum And Investigation Of Traf6’S Role In Proliferation Of Hemocytes In Response To Infection, Jacquelyn T. May

Electronic Theses and Dissertations

Amblyomma americanum is a medically important vector in the southeast United States. Cellular and molecular aspects of the immune system of this species were examined. To investigate cellular processes, hemocytes produced in response to gram-negative infection were characterized according to their ultrastructure. Four hemocyte types were identified: prohemocytes, plasmatocytes, type-I granulocytes, and type-II granulocytes. To elucidate molecular processes, we investigated AamTRAF as an immune-related gene by silencing the gene using RNAi then quantifying the hemocytes after inoculation with gram-negative and positive bacteria. It was found that silencing AamTRAF caused a decrease in hemocyte proliferation in response to gram-positive but not …


The Bees Of Two Sites In The Big Thicket National Preserve, Texas, With A Consideration Of The Effects Of A Rare Flooding Event, Archie Ray Sauls Jr. May 2021

The Bees Of Two Sites In The Big Thicket National Preserve, Texas, With A Consideration Of The Effects Of A Rare Flooding Event, Archie Ray Sauls Jr.

Electronic Theses and Dissertations

Native bees are an important part of terrestrial ecosystems due to their coevolution with flowering plants. This study catalogued the bee fauna of two sandyland sites in the Big Thicket National Preserve and assessed whether a community was impacted by a historic hurricane-induced flooding event. It was hypothesized that a change in diversity metrics would be evident following the flood. Datasets were analyzed for differences in species richness, abundance, evenness, and Shannon’s diversity. Similarities between datasets were also assessed using the multivariate tests analysis of similarity (ANOSIM) and similarity of percentages (SIMPER). At two sites over two years of sampling …


Effects Of Anthropogenic Light And Noise On Anuran Breeding Behavior, Ashley Kobisk May 2021

Effects Of Anthropogenic Light And Noise On Anuran Breeding Behavior, Ashley Kobisk

Electronic Theses and Dissertations

The natural environment can be negatively impacted by a variety of human activities, including the production of artificial light at night and anthropogenic noise. Recent studies suggest that pollution from anthropogenic light and noise alters animal behavior. Despite being highly nocturnal and vocal animals, little attention has been given to anurans and the effects artificial light at night and anthropogenic noise have on their behavior. This study investigated the effects of artificial light at night and anthropogenic noise on anuran breeding systems in eastern Texas. Specifically, this study investigated whether (1) artificial light and anthropogenic noise altered calling behavior in …


Glare Reduction By Dark Facial Markings And Bills In Birds, Clara Lebow May 2020

Glare Reduction By Dark Facial Markings And Bills In Birds, Clara Lebow

Electronic Theses and Dissertations

Avian facial plumage, bill coloration, and feather microstructure may serve one or more adaptive functions. Several researchers have proposed that dark eyestripes, bills, and facial masks aid in reducing glare, however, there have been relatively few tests of this hypothesis. Dark facial markings have been shown to have an adaptive glare-reduction function in recent field studies of a few species, but this hypothesis has never been tested in a broad multispecies analysis. It is likely that feather microstructure influences feather brightness and has an effect on the efficacy of glare reduction properties of feathers. I examined the link between dark …


Bryophyte Assemblages On American Hornbeam (Carpinus Caroliniana) And The Surrounding Arboreal Communities Within The Forest Of East Texas, Cassey Edwards Dec 2018

Bryophyte Assemblages On American Hornbeam (Carpinus Caroliniana) And The Surrounding Arboreal Communities Within The Forest Of East Texas, Cassey Edwards

Electronic Theses and Dissertations

The bryophytes, the non-vascular plants, include the mosses (Bryophyta with approximately 13,000 species), the liverworts (Marchantiophyta with approximately 5,000 species), and the hornworts (Anthocerotophyta with approximately 150 species). Initial observations indicated that the midstory tree Carpinus caroliniana Walter (American hornbeam) has a bryophyte composition with an unusually high diversity of leafy liverworts. Voucher samples were taken from the four intercardinal directions (Northeast, Northwest, Southwest, and Southeast) from six Carpinus trees and their nearest neighbor that was neither Pinus nor another Carpinus at each of six sites across East Texas. Percent coverage, species richness, Shannon’s diversity and Pielou’s evenness were computed. …


Changes In The Microbial Community Of Lubomirskia Baicalensis Affected By Red Sponge Disease, Colin Rorex May 2018

Changes In The Microbial Community Of Lubomirskia Baicalensis Affected By Red Sponge Disease, Colin Rorex

Electronic Theses and Dissertations

Lake Baikal is the oldest known lake and a unique ecosystem, home to several species of fresh water sponge. A disease outbreak affecting the dominant species, Lubormirskia baialensis, was recently reported. The cause of the disease has not been determined but one of the current hypothesis is that the increase in methane concentration is correlated to the disease outbreak. This pilot study characterized the microbiomes of sick and healthy sponges through the use of 16S rRNA sequencing. Sick sponge microbiomes shared a conserved group of taxa while the healthy sponge microbiomes had greater diversity. Indicator species analysis identified two significant …


Vegetation Community Changes In Two National Forests In The Pineywoods, East Texas, Trisha L. Williams May 2017

Vegetation Community Changes In Two National Forests In The Pineywoods, East Texas, Trisha L. Williams

Electronic Theses and Dissertations

Despite extensive research into forest succession, little research has been directed to long-term studies. The fundamental properties of succession remain unclear and further research into terrestrial vegetation and an accounting of drivers in specific ecosystem types is required. This study addresses change in plant communities from five ecosystem types in two east Texas National Forests over a 20-year period. An analysis of 30 sample stands yields results due to various ecosystem drivers of vegetation change and uncovers plant community responses in multiple ecosystem types over this period. This research provided three key results: 1) that vegetation composition change occurs more …