Life Sciences Commons^™

Influence Of Calcareous Soil On Cry3bb1 Expression And Efficacy In The Field, David S. Wangila, Arnubio Valencia, Haichuan Wang, Blair D. Siegfried, Lance Meinke

Department of Entomology: Faculty Publications

Greater than expected injury by western corn rootworm (WCR) (Diabrotica virgifera virgifera LeConte) to Cry3Bb1 expressing maize hybrids (Zea mays L.) has been reported in southwestern Nebraska. Affected areas of some fields are often associated with high pH calcareous soils where maize growth is poor and iron chlorosis is common. As part of a comprehensive study to understand potential causes of unexpected injury, experiments were conducted during 2013 and 2014 to ascertain whether the calcareous soil conditions and associated poor maize growth negatively affect the expression of Cry3Bb1. Quantitative determination of Cry3Bb1 protein expression levels in root tissues …