Life Sciences Commons^™

Quantitative Proteomic And Metabolomic Profiling Reveals Altered Mitochondrial Metabolism And Folate Biosynthesis Pathways In The Aging Drosophila Eye, Hana Hall, Bruce R. Cooper, Guihong Qi, Aruna B. Wijeratne, Amber L. Mosley, Vikki M. Weake

Department of Biochemistry Faculty Publications

Aging is associated with increased risk of ocular disease, suggesting that age-associated molecular changes in the eye increase its vulnerability to damage. Although there are common pathways involved in aging at an organismal level, different tissues and cell types exhibit specific changes in gene expression with advanced age. Drosophila melanogaster is an established model system for studying aging and neurodegenerative disease that also provides a valuable model for studying age-associated ocular disease. Flies, like humans, exhibit decreased visual function and increased risk of retinal degeneration with age. Here, we profiled the aging proteome and metabolome of the Drosophila eye and …

Blue Light Induces A Neuroprotective Open Access Gene Expression Program In Drosophila Photoreceptors, Hana Hall, Jingqun Ma, Sudhanshu Shekhar, Walter D. Leon-Salas, Vikki M. Weake

Department of Biochemistry Faculty Publications

Background: Light exposure induces oxidative stress, which contributes to ocular diseases of aging. Blue light provides a model for light‑induced oxidative stress, lipid peroxidation and retinal degeneration in Drosophila melanogaster. In contrast to mature adults, which undergo retinal degeneration when exposed to prolonged blue light, newly‑eclosed fies are resistant to blue light‑induced retinal degeneration. Here, we sought to characterize the gene expression programs induced by blue light in fies of diferent ages to identify neuroprotective pathways utilized by photoreceptors to cope with light‑induced oxidative stress.

Results: To identify gene expression changes induced by blue light exposure, we profled the nuclear …

Proper Splicing Contributes To Visual Function In The Aging Drosophila Eye, Rachel Stegeman, Hana Hall, Spencer E. Escobedo, Henry C. Chang, Vikki M. Weake

Department of Biochemistry Faculty Publications

Changes in splicing patterns are a characteristic of the aging transcriptome; however, it is unclear whether these age‐related changes in splicing facilitate the progressive functional decline that defines aging. In Drosophila, visual behavior declines with age and correlates with altered gene expression in photoreceptors, including downregulation of genes encoding splicing factors. Here, we characterized the significance of these age‐regulated splicing‐associated genes in both splicing and visual function. To do this, we identified differential splicing events in either the entire eye or photoreceptors of young and old flies. Intriguingly, aging photoreceptors show differential splicing of a large number of visual function …

A Programmable Optical Stimulator For The Drosophila Eye, Xinping Chen, Walter D. Leon-Salas, Taylor Zigon, Donald F. Ready, Vikki M. Weake

Department of Biochemistry Faculty Publications

A programmable optical stimulator for Drosophila eyes is presented. The target application of the stimulator is to induce retinal degeneration in fly photoreceptor cells by exposing them to light in a controlled manner. The goal of this work is to obtain a reproducible system for studying age-related changes in susceptibility to environmental ocular stress. The stimulator uses light emitting diodes and an embedded computer to control illuminance, color (blue or red) and duration in two independent chambers. Further, the stimulator is equipped with per-chamber light and temperature sensors and a fan to monitor light intensity and to control temperature. An …