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Inheritance Of Dhurrin Content In Mature Sorghum Leaves, Herman J. Gorz, Francis A. Haskins, K. P. Vogel
Inheritance Of Dhurrin Content In Mature Sorghum Leaves, Herman J. Gorz, Francis A. Haskins, K. P. Vogel
Department of Agronomy and Horticulture: Faculty Publications
Seedlings of both KS8 and N32 sorghum [Sorghum bicolor (L.) Moench] were high in dhurrin [p-hydroxy-(S)-mandelonitrile-β-D-glucoside] and thus in hydrocyanic acid potential (HCN-p), but the HCN-p of mature leaves from field-grown plants of KS8 was only about one-tenth as high as that of N32. A study of the inheritance of this large difference between KS8 and N32 revealed that a single major gene pair was responsible. There were no obvious maternal effects and F,'s were generally intermediate in HCN-p level between the two parents, indicating that neither high nor low HCN-p was completely dominant. HCN-p …
Relationship Between Contents Of Leucoanthocyanidin And Dhurrin In Sorghum Leaves, Francis A. Haskins, Herman J. Gorz
Relationship Between Contents Of Leucoanthocyanidin And Dhurrin In Sorghum Leaves, Francis A. Haskins, Herman J. Gorz
Department of Agronomy and Horticulture: Faculty Publications
Flag leaves of 'Colman' forage sorghum (Sorghum bicolor) contain at least 25 times as much leucoanthocyanidin (LAC) and approximately half as much of the cyanogenic glucoside, dhurrin, as do flag leaves of 'White Collier' forage sorghum. Assays of flag leaves from 119 F2 plants and 11 F, lines from crosses between these two cultivars revealed a statistically significant negative association between levels of LAC and dhurrin. Both LAC and dhurrin are aromatic compounds, and the negative association between the two may be the result of competition for intermediates or products of the aromatic biosynthetic pathway. This rationale appears …
Influence Of Sample Treatment On Apparent Hydrocyanic Acid Potential Of Sorghum Leaf Tissue, Francis A. Haskins, Herman J. Gorz, R. M. Hill, J. Brakke Youngquist
Influence Of Sample Treatment On Apparent Hydrocyanic Acid Potential Of Sorghum Leaf Tissue, Francis A. Haskins, Herman J. Gorz, R. M. Hill, J. Brakke Youngquist
Department of Agronomy and Horticulture: Faculty Publications
When dhurrin [p-hydroxy-(S)-mandelonitrile-β-D-glucoside], the cyanogenic glucoside of sorghum [Sorghum bicolor (L.) Moench], is hydrolyzed by autoclaving, p-hydroxybenzaldehyde (p-HB) is released. The spectrophotometric determination of p-HB concentration in autoclaved sorghum leaf extracts provides a measure of the hydrocyanic acid potential (HCN-p) of leaf tissue. Extracts of field-grown sorghum leaves contained substances that interfered with this procedure, but ether extraction effectively separated p-HB from these interfering materials. We observed that when flag leaf tissue from field-grown sorghum was dried at 75°C and then autoclaved, HCN-p values were about three times as high …
Effect Of Freezing On The Hydrocyanic Acid Potential Of Field-Grown Sorghum Tillers, Francis A. Haskins, Herman J. Gorz, R. M. Hill, J. Brakke Youngquist
Effect Of Freezing On The Hydrocyanic Acid Potential Of Field-Grown Sorghum Tillers, Francis A. Haskins, Herman J. Gorz, R. M. Hill, J. Brakke Youngquist
Department of Agronomy and Horticulture: Faculty Publications
Tissue disruption caused by freezing and thawing may contribute to rapid enzymatic breakdown of dhurrin [p-hydroxy-(S)mandelonitrile-β-D-glucoside], the cyanogenic glucoside of sorghum [Sorghum bicolor (L.) Moench], but published reports are not in agreement as to the effects of freezing on the hydrocyanic acid potential (HCN-p) of field-grown sorghum leaves. These effects were investigated in a series of experiments in the fan of 1983, a period during which weather conditions at Lincoln, NE, permitted repeated sampling of frozen and non-frozen leaves of field-grown sorghum tillers. Assays of these samples, both spectrophotometrically and with the cyanide electrode, indicated …