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Notch Family Members Follow Stringent Requirements For Intracellular Domain Dimerization At Sequence-Paired Sites, Jacob J. Crow, Allan R. Albig
Notch Family Members Follow Stringent Requirements For Intracellular Domain Dimerization At Sequence-Paired Sites, Jacob J. Crow, Allan R. Albig
Biology Faculty Publications and Presentations
Notch signaling is essential for multicellular life, regulating core functions such as cellular identity, differentiation, and fate. These processes require highly sensitive systems to avoid going awry, and one such regulatory mechanism is through Notch intracellular domain dimerization. Select Notch target genes contain sequence-paired sites (SPS); motifs in which two Notch transcriptional activation complexes can bind and interact through Notch’s ankyrin domain, resulting in enhanced transcriptional activation. This mechanism has been mostly studied through Notch1, and to date, the abilities of the other Notch family members have been left unexplored. Through the utilization of minimalized, SPS-driven luciferase assays, we were …