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Full-Text Articles in Life Sciences
Positional Plasticity In Regenerating Amybstoma Mexicanum Limbs Is Associated With Cell Proliferation And Pathways Of Cellular Differentiation, Catherine D. Mccusker, Antony Athippozhy, Carlos Diaz-Castillo, Charless Fowlkes, David M. Gardiner, S. Randal Voss
Positional Plasticity In Regenerating Amybstoma Mexicanum Limbs Is Associated With Cell Proliferation And Pathways Of Cellular Differentiation, Catherine D. Mccusker, Antony Athippozhy, Carlos Diaz-Castillo, Charless Fowlkes, David M. Gardiner, S. Randal Voss
Biology Faculty Publications
BACKGROUND: The endogenous ability to dedifferentiate, re-pattern, and re-differentiate adult cells to repair or replace damaged or missing structures is exclusive to only a few tetrapod species. The Mexican axolotl is one example of these species, having the capacity to regenerate multiple adult structures including their limbs by generating a group of progenitor cells, known as the blastema, which acquire pattern and differentiate into the missing tissues. The formation of a limb regenerate is dependent on cells in the connective tissues that retain memory of their original position in the limb, and use this information to generate the pattern …
Gene Expression During The First 28 Days Of Axolotl Limb Regeneration I: Experimental Design And Global Analysis Of Gene Expression, S. Randal Voss, Alex Palumbo, Radha Nagarajan, David M. Gardiner, Ken Muneoka, Arnold J. Stromberg, Antony T. Athippozhy
Gene Expression During The First 28 Days Of Axolotl Limb Regeneration I: Experimental Design And Global Analysis Of Gene Expression, S. Randal Voss, Alex Palumbo, Radha Nagarajan, David M. Gardiner, Ken Muneoka, Arnold J. Stromberg, Antony T. Athippozhy
Biology Faculty Publications
While it is appreciated that global gene expression analyses can provide novel insights about complex biological processes, experiments are generally insufficiently powered to achieve this goal. Here we report the results of a robust microarray experiment of axolotl forelimb regeneration. At each of 20 post-amputation time points, we estimated gene expression for 10 replicate RNA samples that were isolated from 1 mm of heterogeneous tissue collected from the distal limb tip. We show that the limb transcription program diverges progressively with time from the non-injured state, and divergence among time adjacent samples is mostly gradual. However, punctuated episodes of transcription …