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Systematic Changes In Gene Expression Patterns Following Adaptive Evolution In Yeast, Tracy L. Ferea, David Botstein, Patrick O. Brown, R. Frank Rosenzweig
Systematic Changes In Gene Expression Patterns Following Adaptive Evolution In Yeast, Tracy L. Ferea, David Botstein, Patrick O. Brown, R. Frank Rosenzweig
Biological Sciences Faculty Publications
Culturing a population of Saccharomyces cerevisiae for many generations under conditions to which it is not optimally adapted selects for fitter genetic variants. This simple experimental design provides a tractable model of adaptive evolution under natural selection. Beginning with a clonal, founding population, independently evolved strains were obtained from three independent cultures after continuous aerobic growth in glucose-limited chemostats for more than 250 generations. DNA microarrays were used to compare genome-wide patterns of gene expression in the evolved strains and the parental strain. Several hundred genes were found to have significantly altered expression in the evolved strains. Many of these …