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Western Michigan University

Honors Theses

Hereditary fructose intolerance

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Glut5 And Fructose Metabolism Inhibition In A Simulated Small Intestine Environment, Isabella Trainor Apr 2020

Glut5 And Fructose Metabolism Inhibition In A Simulated Small Intestine Environment, Isabella Trainor

Honors Theses

This thesis describes an experiment that was designed in order to obtain information that could be useful for treating individuals with HFI. The author hypothesized that MSNBA would inhibit GLUT5 and fructose metabolism in the small intestine. The final experimental design involves exposing a small intestine cell line to fructose and MSNBA. The cell line would be exposed to MSNBA over the course of three different periods, and data was intended to be obtained using western blotting and a fructose assay kit. Although the final experimental design project was not fully carried out due to circumstances surrounding COVID-19, a plausible …