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Full-Text Articles in Life Sciences
Trypanosoma Cruzi Prevalence In The Domestic Canine Population In Central And Eastern Kentucky, Katelyn Cox
Trypanosoma Cruzi Prevalence In The Domestic Canine Population In Central And Eastern Kentucky, Katelyn Cox
Mahurin Honors College Capstone Experience/Thesis Projects
Domestic canines are regarded as natural sentinelsfor the transmission of vector-borne pathogens since infection in an owner’s dog suggests the presence of the vector in or around the household. In collaboration with the University of Kentucky’s Veterinary Diagnostic Lab in Lexington, Kentucky, we have investigated the prevalence of Trypanosoma cruzi (the causative agent of Chagas disease) in canines from central and eastern Kentucky via a serological test, polymerase chain reaction (PCR), and DNA sequencing. In addition, Canine SNAP 4Dx plus tests (Idexx Laboratories, Inc.) were used to determine the prevalence of four other vector-borne pathogens: Ehrlichia canis/ewingii, Borrelia burgdorferi, Anaplasma …
Molecular Analysis Of Trypanosoma Cruzi Isolates Obtained From Raccoons (Procyon Lotor) In Warren And Barren Counties Of Kentucky, Lipeng Bi
Masters Theses & Specialist Projects
Trypanosoma cruzi, the etiologic agent of Chagas disease, infects a variety of wild mammals in the southern United States, but it has only recently been isolated from raccoons trapped in the state of Kentucky. The purpose of the present study was to use a molecular genotyping approach, followed by DNA sequencing to determine the genotypes (type I, or types IIa-IIe) of 15 of the Kentucky isolates. DNA samples were prepared from 15 T. cruzi- isolates using a Qiagen mini kit, and PCR amplification was performed using published primers for the 24S α rDNA sequence (D71 and D72), the …
Trypanosoma Cruzi In Wild Raccoons And Opossums From Kentucky, Brian Chad Groce
Trypanosoma Cruzi In Wild Raccoons And Opossums From Kentucky, Brian Chad Groce
Masters Theses & Specialist Projects
Only 6 autochthonous cases of human Chagas disease have been documented in the U.S.A., however, as many as 5% of immigrants from Latin America may be infected with the etiologic agent, Trypanosoma cruzi. The parasite has been isolated from a variety of wild mammals, particularly in the southeastern region of the U.S.A. The goal of our study was to determine if the sylvatic cycle of T. cruzi infection occurs in Kentucky, and, if present, to assess the prevalence of infection in Warren and Barren counties. Raccoons and opossums were live-trapped between June and December, 2007. Animals were anesthetized with …