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Full-Text Articles in Life Sciences
Action Spectrum For Photoentrainment Of The Circadian Clock In Wild-Type Chlamydomonas Reinhardtii, Jennifer Forbes=Stovall
Action Spectrum For Photoentrainment Of The Circadian Clock In Wild-Type Chlamydomonas Reinhardtii, Jennifer Forbes=Stovall
Masters Theses & Specialist Projects
The circadian clock is an endogenous timer that oscillates with a period of approximately 24 hours and is reset upon environmental time cues such as the daily light/ dark or temperature cycles. Chlamydomonas reinhardtii is an ideal model organism for research on the circadian clock, because it shows several well-characterized behaviors that exhibit a circadian rhythm. Its circadian rhythm of phototaxis (swimming toward light) has been automated. Former action spectrum studies using the circadian phototaxis rhythm as an indicator surprisingly found that pulses of blue light were not effective in resetting the circadian clock of dark-adapted cells. This may have …
Screening Rnai Transformants Of Chlamydomonas For Reduced Expression Of The Photoreceptor Cryptochrome, Jeremy Webb
Screening Rnai Transformants Of Chlamydomonas For Reduced Expression Of The Photoreceptor Cryptochrome, Jeremy Webb
Mahurin Honors College Capstone Experience/Thesis Projects
Chlamydomonas reinhardtii is a unicellular green alga. In C. reinhardtii, three types of photoreceptors are known to be present: rhodopsins, phototropins, and cryptochromes. The single cryptochrome is the most likely photoreceptor for adjusting the circadian clock to the daily light/dark cycles, because cryptochromes are involved in clock entrainment in higher plants and insects. In this segment of the research, C. reinhardtii strains, which were genetically modified through transformation with a RNA interference construct, were screened for reduction in cryptochrome compared to the control strain. After C. reinhardtii cultures were harvested in complete darkness, all soluble proteins were extracted from the …