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Full-Text Articles in Life Sciences
Role Of Cd4+Cd25+ Regulatory T Lymphocytes In Experimental Toxoplasmosis, Sanjay Varikuti
Role Of Cd4+Cd25+ Regulatory T Lymphocytes In Experimental Toxoplasmosis, Sanjay Varikuti
Masters Theses & Specialist Projects
Toxoplasmosis is an important cause of congenital disease, and it is one of the most common opportunistic infections in patients with acquired immunodeficiency syndrome. The need for a reliable experimental model of this infection is crucial not only for achieving a better understanding of the patho-physiology of the disease, but also for developing better methods for evaluating new therapeutic regimens. The purpose of the present study was to investigate the role of CD4+CD25+ T regulatory lymphocytes in mice infected with Toxoplasma gondii. T regulatory (Treg) cells have been shown to play an important role in our immune system in …
Quantum Dot Immuno-Conjugates Allow For Reliable Photo-Stable Detection Of Intracellular Stages Of Toxoplasma Gondii, Natalia Barahona Guerrero
Quantum Dot Immuno-Conjugates Allow For Reliable Photo-Stable Detection Of Intracellular Stages Of Toxoplasma Gondii, Natalia Barahona Guerrero
Mahurin Honors College Capstone Experience/Thesis Projects
Luminescent quantum dot nanoparticles have emerged as a highly effective alternative to organic fluorescence probes in a variety of applications employing the use of immunoconjugates. In the present study, we have successfully used quantum dot technology for the immunofluorescent detection of intracellular stages of Toxoplasma gondii in a CV-1 mammalian cell line. This assay will allow us to investigate the question of whether selenium and other antioxidants have a direct impact on the intracellular cycle of the parasite. A Lab-Tek cell culture system was used to determine optimal concentrations of primary and secondary antibodies. Infected CV-1 monolayers were fixed in …