Life Sciences Commons^™

The Art Of A Slugslayer: An Exploration Of The Process Of Extracting Radulae From Lehmannia Valentiana For The Purpose Of Viewing In The Scanning Electron Microscope, Elizabeth Gannon

Mahurin Honors College Capstone Experience/Thesis Projects

A radula is an anatomical structure found exclusively in mollusks that acts as both tongue and teeth in feeding. Its structure has been studied for more than a century, yet published methods for identifying, dissecting, and cleaning radulae for microscopy are almost unintelligible to anyone not already familiar with molluscan anatomy. The purpose of this project was to identify the best method of garden slug radula isolation and present it in a manner that anyone could understand and successfully use.

After identifying the location of the radula in the slug, the easiest means of removal proved to be simply regurgitating …

Growth And Survival Of Amphibians Exposed To Road Salt And Glyphosate-Based Herbicide, Jerica Eaton

Mahurin Honors College Capstone Experience/Thesis Projects

Amphibian populations are threatened by increasing levels of environmental pollution resulting from human activities. Areas with mixed land use that include agriculture and urban development experience increased contamination from surface herbicide and road deicer application. Upon entering water sources, these chemicals have the capacity to disrupt the natural dynamics of aquatic ecosystems. This study aimed to evaluate the effects of these contaminants on amphibian populations. I used the spotted salamander (Ambystoma maculatum) as a model, rearing larvae from hatching to metamorphosis. I subjected larvae in each of the treatment groups to varying levels of herbicide and salt concentrations. …

Teloportwrapper: A New Tool For Understanding The Dynamic World Of Fungal Telomere Ends, Trey Stansfield

Mahurin Honors College Capstone Experience/Thesis Projects

Telomeres are repetitive DNA sequence motifs found at eukaryote chromosome ends. Telomeres help protect chromosome ends from DNA damage and promote chromosome stability. Chromosomes play important roles in aging, mutation, and cancer. Eukaryotic pathogens also use telomeres to mutate and manage virulence genes. In response to chromosome end breakage newly formed telomeres, called de novo telomeres, are formed to recreate the lost telomere and sub-telomeric regions.

Magnaporthe oryzae is a fungal pathogen which causes wheat blast, a deadly plant disease in wheat. Magnaporthe oryzae is also known for its highly variable sub-regions which show high amounts of induced variability due …

Comparison Of Groundwater Chemistry And Associated Biodiversity Of Sulfidic Karst Habitats In Southcentral Kentucky, Kendall Wheeler

Mahurin Honors College Capstone Experience/Thesis Projects

In most ecosystems, primary production is conducted by plants or photosynthetic microbes; however, extremophilic sulfur-oxidizing bacteria can use hydrogen sulfide gas (H2S) as an electron donor to produce metabolic energy in a type of metabolism called chemoautotrophy. These ecosystems are a prominent feature of some karst landscapes, and two have been observed in the Mammoth Cave system, Sulphur River in Parker Cave and Marianne’s Pass in Mammoth Cave National Park respectively.

Sulfidic water is ubiquitous in southcentral Kentucky and is strongly associated with hydrocarbon deposits. This oil boom of the 1880s in this region predates most environmental regulation and records …