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Full-Text Articles in Life Sciences
Correlation Of Visualized Glucocorticoid Receptor And Apoptosis In Individual Clones Of Nb2 Cells, Devang M. Patel
Correlation Of Visualized Glucocorticoid Receptor And Apoptosis In Individual Clones Of Nb2 Cells, Devang M. Patel
Theses and Dissertations
The Nb2 lymphoma cell line is susceptible to glucocorticoid (GC)-induced apoptosis in the absence of mitogen and this effect is inhibited by the mitogen prolactin (PRL). This process is believed to be mediated by the glucocorticoid receptor (GR) and various downstream signals. The purpose of this study was to use the limiting dilution method to isolate subsets of Nb2 clones which expressed either highly enriched amounts of GR or an absence of GR and to relate the amount of GR to the magnitude of GC induced cytolysis. The presence of GR in these clones was determined by immunocytochemistry (ICC) using …
Characterization Of Rat Pulmonary Carboxylesterase, Timothy J. Wallace
Characterization Of Rat Pulmonary Carboxylesterase, Timothy J. Wallace
Theses and Dissertations
The 1839 bp cDNA for rat pulmonary carboxylesterase was cloned by reverse transcription polymerase chain reaction (RT PCR) from total rat lung RNA using specific primers derived from the 5' and 3' untranslated regions of rat hepatic cholesteryl ester hydrolase (CEH). The unique cDNA was sequenced and found to have 99% homology with hepatic CEH. This homology extends to the predicted amino acid sequences which show only six amino acid residue differences in the coding region: three conserved and three nonconserved changes. However, the catalytic activitites of the two proteins are dramatically different. While CEH hydrolyzes cholesterol oleate, the pulmonary …
The Roles Of Several Kinases In Mice Tolerant To Delta-9-Tetrahydrocannabinol, Matthew C. Lee
The Roles Of Several Kinases In Mice Tolerant To Delta-9-Tetrahydrocannabinol, Matthew C. Lee
Theses and Dissertations
It has been suggested that the CB1 G-protein-coupled receptor is internalized following agonist binding and activation of the second messenger pathways. The process of desensitization and resensitization is intimately involved with receptor internalization. Phosphorylation alters tolerance to cannabinoids thus contributing to tolerance. It is proposed that phosphorylation enhances the down-regulation of the CB1 receptor. These findings led us to look at which kinase(s) may be involved in cannabinoid tolerance. We therefore hypothesize that by preventing phosphorylation of the CB1 receptor, we may reverse tolerance. We evaluated our hypothesis by testing the role of several kinases in tolerance: protein kinase A …
Examination Of Signals Involved In Dexamethasone Induced Apoptosis In Nb2 Lymphoma Cells, Suhas Badarinath
Examination Of Signals Involved In Dexamethasone Induced Apoptosis In Nb2 Lymphoma Cells, Suhas Badarinath
Theses and Dissertations
Consistent with previous studies, we demonstrated that dexamethasone (Dex) caused cytolysis/apoptosis in log phase Nb2 lymphoma cells, while prolactin (Prl) inhibited this effect. The Nb2 model was used to investigate the mechanisms of apoptosis control with the aid of immunocytochemistry (ICC). We established with absorption the specificity of staining due to Fas, Fas Ligand, Bcl-2, and Bax antibodies (the specificity of anti-p53 could not be verified). Dex-induced cytolysis/apoptosis was detected in synchronized (G⁰/G¹) cells after 6 and 8 hours of Dex exposure. A novel, computerized technique was used to quantitate the proportion of cells immunostained for the signals of interest …