Life Sciences Commons^™

Fruits Of Nandina Domestica Are (Sometimes) Cyanogenic And (Sometimes) Hazardous To Birds, Scott Zona

Poisonous Plant Research (PPR)

A 12-month survey of cyanogenesis in the fruits and leaves of Nandina domestica (Berberidaceae) revealed that the leaves are strongly cyanogenic throughout the year, as measured by the Feigl-Anger test. Fruits vary in their cyanogenic potential depending on the degree of ripeness and clone. Green fruits are strongly and rapidly cyanogenic, but most ripe fruits are weakly and slowly cyanogenic. Some fruits tested negative for cyanide. Fruits tend to become less cyanogenic the longer they remain on the plant, so birds feeding on older fruits are at a lower risk of poisoning. More than other frugivores, cedar waxwings (Bombycilla …

The Effects Of Zno Nanoparticles On Egg, Larva, And Adult Rough-Skinned Newts (Taricha Granulosa), Austin Reid Spence

Undergraduate Honors Capstone Projects

The objective of this study was to examine the effects of zinc oxide (ZnO) nanoparticles on egg, larva, and adult rough-skinned newts, Taricha granulosa. To date, little research has been done to investigate the potentially detrimental effects of nanoparticles on amphibians, especially salamanders and newts (caudates). Chronic toxicity was tested on eggs and larvae, and acute toxicity was tested on eggs, larvae, and adults. For eggs, chronic exposure to ZnO nanoparticles caused higher mortality at 10.0 and 100.0 mg/L compared to 0.0, 0.1, and 1.0 mg/L. When given an acute exposure (24h) to nanoparticles at a late incubation stage, …

Effects Of Condensed Tannins On The Toxicity Of Fireweed (Senecio Madagascariensis) To Cattle, Carolyn L. Wong

All Graduate Theses and Dissertations, Spring 1920 to Summer 2023

Fireweed (Senecio madagascariensis) is a noxious and invasive weed affecting pastures in Hawaii, Australia, and South America. Fireweed contains compounds called pyrrolizidine alkaloids that are toxic to most grazing mammals. Toxic effects to cattle include irreversible damage to liver cells, hardening of the liver, and loss of liver function, which may lead to jaundice, swelling, and the accumulation of fluids in the stomach and other physiological malfunctions. External effects include rough appearance, diarrhea, low energy and dullness, photosensitization, and abnormal behavior, many of which can lead to death. Fireweed also can reduce pasture productivity by as much as …

The Distribution Of Fruit And Seed Toxicity During Development For Eleven Neotropical Trees And Vines, Noelle G. Beckman

Biology Faculty Publications

Secondary compounds in fruit mediate interactions with natural enemies and seed dispersers, influencing plant survival and species distributions. The functions of secondary metabolites in plant defenses have been well-studied in green tissues, but not in reproductive structures of plants. In this study, the distribution of toxicity within plants was quantified and its influence on seed survival was determined in Central Panama. To investigate patterns of allocation to chemical defenses and shifts in allocation with fruit development, I quantified variation in toxicity between immature and mature fruit and between the seed and pericarp for eleven species. Toxicity of seed and pericarp …

Investigation Of The Toxicity And Toxicokinetics Of Selenium From The Accumulator Plant Symphyotrichum Spathulatum (Western Mountain Aster) In Sheep, Amanda Wilhelm

All Graduate Theses and Dissertations, Spring 1920 to Summer 2023

This study was designed to observe the effects of selenium from plant material in sheep after a single, oral dose. Purified sodium selenite and selenomethionine were given as positive controls. The plant Symphyotrichum spathulatum (Western Mountain Aster) was collected, analyzed for selenium content, and administered orally to sheep at varying doses according to body weight. Clinical signs were observed for 7 days during which time whole blood, serum, and expired air were collected. Following euthanasia, tissues were collected for histopathological analysis and mineral analysis.

Clinical signs were less apparent than expected and included depression and mild dyspnea in sheep receiving …

Treatment Of Late Stage Disease In A Model Of Arenaviral Hemorrhagic Fever: T-705 Efficacy And Reduced Toxicity Suggests An Alternative To Ribavirin, B B. Gowen, D F. Smee, M H. Wong, J O. Hall, K H. Jung, K W. Bailey, J R. Stevens, Y Furuta, John D. Morrey

John D. Morrey

A growing number of arenaviruses are known to cause viral hemorrhagic fever (HF), a severe and life-threatening syndrome characterized by fever, malaise, and increased vascular permeability. Ribavirin, the only licensed antiviral indicated for the treatment of certain arenaviral HFs, has had mixed success and significant toxicity. Since severe arenaviral infections initially do not present with distinguishing symptoms and are difficult to clinically diagnose at early stages, it is of utmost importance to identify antiviral therapies effective at later stages of infection. We have previously reported that T-705, a substituted pyrazine derivative currently under development as an anti-influenza drug, is highly …

Investigation Of The Toxic Affect Of Manganese On Ruminal Microbial Digestion And Growth, Erin Dearing

Undergraduate Honors Capstone Projects

This research investigated the toxic effect of manganese, in a reduced chemical state, on rumen microbial metabolism and growth. The reduced form of manganese is the soluble state found in subsurface waters and has a high red-ox potential. In the study, we investigated the effects of reduced manganese at varying concentrations, on rumen microbial ability to replicate and metabolize carbohydrates. The hypotheses that we tested were as follows: (1) reduced manganese decreases the rate of pH drop associated with rumen culture fermentation of a com based diet and (2) reduced manganese decreases the bacterial growth in rumen culture, as measured …

Bioavailability/Toxicity Of Iron From Aerobically Processed Organic Fertilizer, Stacey Marie Wilson

Undergraduate Honors Capstone Projects

For an undergraduate Honor's project, I worked under the direction of Dr. Jeffery Hall to determine the bioavailability and toxicity of iron from Milorganite® fertilizer, an aerobically processed organic fertilizer. The Milorganite® Company is a subsidiary of the Milwaukee Municipal Sewage District and produces a fertilizer of high iron content (approximately 5-7%). The high iron content has resulted in concern about the toxic potential, which until now was unknown. This thesis paper will explain iron chemistry, bioavailability, requirements, absorption, toxicity, and treatments, followed by a description of fertilizer types and contents. This will be followed by a description and reasoning …

Selenium Poisoning Of Wildlife And Western Agriculture: Cause And Effect, N. E. Korte

All U.S. Government Documents (Utah Regional Depository)

This project examined the hypothesis that selenium contamination is not the principal cause of the decline of endemic fish species in the Upper Colorado Basin. Activities employed to test this hypothesis included a reconnaissance of locations altered by recent road construction, a reinterpretation of available literature regarding selenium toxicity, and the interpretation of unpublished data obtained from the Upper Colorado Basin Fish Recovery Program. The project demonstrates that most of the evidence implicating selenium is circumstantial. Specifically, this research demonstrates that neither the historical record nor the technical literature consistently supports the emphasis given selenium toxicity. For example, many locations …

Clinical Trials With Copper Supplementation, Clell Bagley, Norris Stenquist, Dennis Worwood

All Current Publications

Copper deficiency has been diagnosed in beef cattle herds in many areas of the intermountain west. Copper supplements are not widely used, even though several products are available. It is difficult to correct a deficiency because too much copper can result in copper toxicity or poisoning. Toxicity is less a hazard with cattle than with sheep, but it is still a problem to guard against. Periodic monitoring of the herd’s copper status is essential for proper supplementation.

Copper Deficiency In Utah, Clell Bagley, Norris Stenquist, Dennis Worwood

All Current Publications

Copper deficiency has been diagnosed in a number of Utah cattle herds, yet few producers use copper supplements. Cattle deficient in the element can suffer significant production losses. Producers need to balance both the effects and the costs of copper supplements. Too much copper can cause copper toxicity or poisoning. And adding copper may not be economical if animals are only marginally deficient. Further, the deficiency may occur only on specific feeds or pastures, correcting itself when cattle are moved.

Clinical Trials With Copper Supplementation Of Cattle, Clell V. Bagley, Norris J. Stenquist, Dennis R. Worwood

All Archived Publications

No abstract provided.

Toxicity Of Aspen Wood Leachate To Aquatic Life: Laboratory Studies, Barry R. Taylor, J. Stephen Goudey, N. Bruce Carmichael

Aspen Bibliography

Trembling aspen (Populus tremuloides Michx.), a common hardwood tree throughout Canada, is being harvested at increasing rates for use in paper and building materials. Piles of aspen logs have been observed to produce a dark, watery, acutely toxic leachate. A laboratory study was undertaken to elucidate the nature, strength, and persistence of aspen leachate toxicity and the chemical composition of the leachate. Leaching from aspen chips in the laboratory was rapid, with 1% mass loss in the first 24 h. Another 2 weeks of immersion was necessary to remove all remaining leachable material (3% total)> Fresh aspen leachate derived …

Biological Action Of Mycotoxins, Roger A. Coulombe Jr.

Animal, Dairy, and Veterinary Science Faculty Publications

Mycotoxins are ubiquitous, mold-produced toxins that contaminate a wide variety of foods and feeds. Ingestion of mycotoxins cause a range of toxic responses, from acute toxicity to long-term or chronic health disorders. Some mycotoxins have caused outbreaks of human toxicoses, and at least one mycotoxin, aflatoxin B1, is a presumed human hepatocarcinogen. As part of a comprehensive effort to curtail the adverse health effects posed by mycotoxins, substantial research has been conducted to determine the mechanism of action of mycotoxins in animals. This review presents some of the current knowledge on the biological action of four diverse classes of mycotoxins—aflatoxin …

Immunosuppression By Aflatoxin B1 In C57bl/6 Mice And Its Relationship With Neuroendocrine Mechanisms, Yasuhiko Hatori

All Graduate Theses and Dissertations, Spring 1920 to Summer 2023

Aflatoxin B₁ (AFB₁), a secondary metabolite of Aspergillus flavus and Aspergillus parasiticus, is known for its potent carcinogenicity and immunosuppressive effects. It is also known that AFB₁ toxicity appears in different degrees in different animal species and strains.

The present study was performed to reveal the involvement of the hypothalamus-pituitary-adrenal gland (HPA) axis in the immunosuppressive effects of AFB₁ on C57BL/6 mice. Splenic lymphocytes were assayed to investigate their phenotyping using flow cytometry, proliferative response against mitogen and allogenic lymphocytes, cytolytic cell activity, and IL-2 production. In addition, antibody-mediated immunocompetence was checked using sheep …

Carbon Dioxide Toxicity In Wheat, Robert Spanarkel

All Graduate Theses and Dissertations, Spring 1920 to Summer 2023

This research was conducted to quantify short- and long-term effects of atmospheric carbon dioxide on wheat. Growth, development, and yield of the spring wheat cultivar Veery-10 were measured in response to CO₂ concentrations of 340 (ambient), 1200, and 2500 μmol moI^-1 of CO₂ air. These 3 CO₂ levels were chosen to provide a control group, a predicted optimal CO₂ environment, and a potentially toxic CO₂environment, respectively. A recirculating hydroponic system provided a near-optimal root-zone environment that was identical for all CO₂ treatment levels. Environmental factors, other than CO₂, were …

The Response Phase - The First Six Hours After Acute Airway Injury By So2 Inhalation: An In Vivo And In Vitro Study, W. C. Hulbert, S. F. P. Man, M. K. Rosychuk, G. Braybrook, J. G. Mehta

Scanning Microscopy

We have identified an airway epithelial response following acute injury that cannot be termed 'repair' or regeneration. It precedes these well characterized events and it is termed the 'response phase'. We tested the hypothesis that for the first 6 h following acute injury to the tracheal mucosa, the initial cellular events of the response phase will continue as in vivo even if the tissue is maintained in vitro in an Ussing chamber. The tracheal mucosa of anesthetized, intubated mongrel dogs was injured by the inhalation of SO₂ 500 ppm for 1 h (7 dogs); controls (3 dogs) breathed filtered, …

Intracellular Localization Of Heavy Metals In Yeast By X-Ray Microanalysis, Ljerka Kunst, Godfried M. Roomans

Scanning Electron Microscopy

The intracellular localization of heavy metals in yeast cells was studied by means of energy-dispersive X-ray microanalysis. The yeast, Saccharomyces cerevisiae, was pretreated with phosphate and then loaded with different metal ions, by suspending the cells in salt solutions (Ni, Zn, Cd, Pb, Al and Cr). For the analysis, the cells were embedded in gelatin, rapidly frozen, and thin cryosections were cut on a dry knife.

A considerable uptake of divalent cations by the yeast cells was found to occur. The cations were bound to the polyphosphate granules localized in or close to the cell vacuoles. Immediately after phosphate …

Scanning And Transmission Electron Microscopic Studies On The Oviducts Of Pekin Ducks Fed Methyl Mercury Containing Diets, A. Balachandran, M. K. Bhatnagar, H. D. Geissinger

Scanning Electron Microscopy

This study was undertaken to examine the effects of varying levels of methyl mercury (MeHg) on the ultrastructure of the surface epithelium of the oviduct of ducks. Accordingly, Pekin ducks were maintained on feed containing varying doses of (0.0; 0.5; 5.0; 15.0 ppm) of MeHg (Group I - control to IV) for 12 weeks and sacrificed. Tissue from the magnum and the shell gland regions of the oviduct was processed for scanning electron microscopy (SEM) and transmission electron microscopy (TEM). It was found that the primary and secondary folds of these regions of the oviducts of the control and 0.5 …

The Toxicity, Metabolism And Distribution Of Carbaryl In Three Species Of Labops With And Without Piperonyl Butoxide Treatment (Hemiptera:Miridae), Deifalla H. Osman

All Graduate Theses and Dissertations, Spring 1920 to Summer 2023

Carbaryl toxicity, metabolism, and distribution in adults of three species of grass bugs from the genus Labops were studied in relation to species, sex, and treatment with piperonyl butoxide. LC₅₀ values for 8 hour exposure periods ranged from 0.02-0.14, 0.03-0.3, and 0.2-0.7 μg carbaryl/vial for L. utahensis, L. hirtus, and L. hesperius respectively. The males were more susceptible to carbaryl than females. Males of L. utahensis were more susceptible than L. hirtus and L. hesperius respectively.

The synergist difference values (LC₅₀ of carbaryl alone - LC₅₀ values of carbaryl after piperonyl butoxide treatment) were measured. …

Toxicity, Selectivity, And Efficacy Of Squoxin (1,1'-Methylendi-2-Napthol) To Fishes In Utah Waters, Dexter R. Pitman

All Graduate Theses and Dissertations, Spring 1920 to Summer 2023

Squoxin (1,1'-Methylendi-2-napthol) was tested in laboratory and field bioassays to evaluate its biological activity to rainbow trout (Salmo gairdneri), cutthroat trout (Salmo clarki), Utah chub (Gila atraria), carp (Cyprinus carpio), reside shiner (Richardsonius balteatus), and mountain sucker (Catostomus platyrhynchus) under various conditions of water quality and temperature. The compound was toxic to all species and most effective from greatest to least to mountain sucker, reside shiner, Utah chub, cutthroat trout, and rainbow trout and carp. Selectively and safety indices for Utah chub compared with the two species of …

Xenobiotic Detoxication Potential And Drug Induced Changes In Carbaryl Toxicity In The Alfalfa Leafcutter Bee, Megachile Rotundata (Febricius), Richard M. Lee

All Graduate Theses and Dissertations, Spring 1920 to Summer 2023

The alfalfa leafcutter bee, Megachile rotundata, one of the most important pollinators of alfalfa, generally became more susceptible to carbaryl as the adults aged. LD₅₀ values for carbaryl toxicity were 240,166, 109, and 51 μg carbaryl/gram for the 1, 2, 3, and 4 days-old adult male bees. For the female bees, these were 245 , 551, 289, and 262 μg car-baryl/ gram. Lipid content and microsomal enzyme activity (measured by EPN detoxication in vitro) decreased in both sexes as the bees aged.

Synergist ratios, defined as the ratio of the LD₅₀ of carbaryl alone …