Life Sciences Commons^™

Microbiome Assembly And Function In The Solitary Mason Bee, Osmia Lignaria (Megachilidae), Bailey Crowley

All Graduate Theses and Dissertations, Fall 2023 to Present

Animal-microbe interactions can influence host biology, ecology, and evolution. The assembly and function of microbes found within animal hosts oftentimes depends on which species are involved. Advances in sequencing technologies have permitted the exploration of host-microbe interactions in a variety of animals, including bees. Early research aimed at understanding the microbiomes of social bees, such as honey bees and bumble bees, found that microbes prevent the spoilage of stored pollen, breakdown indigestible nutrients into smaller molecules available for uptake by the host, and also protect the host from pathogens. When environmental stressors, such as increased temperatures, disrupt the microbiome, the …

Consumption Of A Western Diet Enhanced Colitis-Associated Colorectal Cancer And Dysbiosis Of The Fecal Microbiome In Mice Notwithstanding Dietary Intervention Or Fecal Microbiome Transfer, Daphne Michelle Rodriguez Jimenez

All Graduate Theses and Dissertations, Spring 1920 to Summer 2023

In a rodent model of inflammation-associated colorectal cancer, consumption of a Western-style diet increases gut inflammation and enhances risk of developing colon tumors. The goal of this dissertation was to understand the contribution of bacteria within the large intestine on colon inflammation and colon tumorigenesis. Two pre-clinical animal studies were performed using two different intervention strategies to shift the microbiome, and potentially gut inflammation and tumor development: 1) an experiment using dietary supplementation with black raspberries, a functional food enriched in bioactive anthocyanins with purported antiinflammatory activity, and 2) an experiment using fecal microbiota transfer from mice fed a healthy …

Changes In Metal-Chelating Metabolites Induced By Drought And A Root Microbiome In Wheat, Anne J. Anderson, Joshua M. Hortin, Astrid R. Jacobson, David W. Britt, Joan E. Mclean

Biological Engineering Faculty Publications

The essential metals Cu, Zn, and Fe are involved in many activities required for normal and stress responses in plants and their microbiomes. This paper focuses on how drought and microbial root colonization influence shoot and rhizosphere metabolites with metal-chelation properties. Wheat seedlings, with and without a pseudomonad microbiome, were grown with normal watering or under water-deficit conditions. At harvest, metal-chelating metabolites (amino acids, low molecular weight organic acids (LMWOAs), phenolic acids, and the wheat siderophore) were assessed in shoots and rhizosphere solutions. Shoots accumulated amino acids with drought, but metabolites changed little due to microbial colonization, whereas the active …

The Effect Of Fish Oil Supplementation And A Western-Type Diet On Gut Health, Marcus Hayden

Fall Student Research Symposium 2022

Colorectal cancer (CRC) is one of the most prevalent cancers worldwide and has increased incidence in more developed countries (Parkin et al., 2005). Diets in more developed and Westernized countries, where CRC is more prevalent, tend to be energy-dense and nutrient deficient. Discovering which food patterns inhibit inflammation matters because the duration of colitis is a risk factor for CRC. Deficiencies in an American diet led to decreased gut microbiota diversity and increased prevalence of colitis. A key component of colon health is the microbiome. Dietary bioactive compounds change the composition of the microbiome. Docosahexaenoic acid (DHA) is a promising …

Go With Your Gut: The Role Of Gut Microbiota In Health, Lydia Bangerter, Mateja R. Savoie-Roskos, Jeffrey Chapman, Abbigail Sundell, Carrie Durward

All Current Publications

Living within your gastrointestinal tract (gut) are about 100 trillion bacterial cells—your gastrointestinal microbiota—a host of bacteria that play a vital role in your body (Ghaisas, Maher, & Kanthasamy, 2016). Your gut microbiome is responsible for a wide variety of functions that contribute to overall health and may be linked to many chronic diseases including cancer, obesity, and cardiovascular disease (Ghaisaset al., 2016; Peregrin, 2013). This fact sheet will discuss the role the gut microbiome plays in various aspects of health and how to improve your gut health.

Molecular Interactions Of Salmonella With The Host Epithelium In Presence Of Commensals, Prerak T. Desai

All Graduate Theses and Dissertations, Spring 1920 to Summer 2023

Food-borne infections are a major source of mortality and morbidity. Salmonella causes the highest number of Food-borne bacterial infections in the US. This work contributes towards developing strategies to control Salmonella by (a) defining receptors used by Salmonella to adhere to and invade the host epithelium; (b) developing a host receptor based rapid detection method for the pathogen in food matrix; (C) and defining mechanisms of how probiotics can help alleviate Salmonella-induced cell death in the host epithelium.

We developed a cell-cell crosslinking method to discover host-microbe receptors, and discovered three new receptor-ligand interactions. Interaction of Salmonella Ef-Tu with …