Life Sciences Commons^™

Microbial Ecology Of An Animal Waste-Fueled Induced Blanket Reactor, Steven C. Curtis

All Graduate Theses and Dissertations, Spring 1920 to Summer 2023

Use of an induced blanket reactor (IBR) to break down organic matter into methane is a financially attractive method to reduce the environmental impact of animal or industrial waste. In order to better understand the biological processes involved with the conversion of waste to biogas by an IBR, it is necessary to gain a better understanding of the microorganisms and their roles in the reactor. Molecular techniques based on the isolation of 16S rDNA were used in order to avoid the limitations posed by conventional culture-based techniques. Total DNA was extracted and amplified using universal primers specific to eubacteria and …