Life Sciences Commons^™

Changes In Sodium Concentration In Cardiac Myocytes From Diabetic Rats, Alice Warley

Scanning Microscopy

The effects of streptozotocin induced diabetes on rats were studied. The animals showed an increase in blood glucose concentration and a loss of weight from both the body and the heart. Loss of weight from the heart was less severe leading to an increased heart to body weight ratio. Study of element concentrations by X-ray microanalysis showed that there was an increase in intracellular Na concentration in cardiac myocytes from the diabetic animals, but no change in Mg. These results agree with studies which show changes in Na/K ATPase after the onset of diabetes.

Microprobe Analyses Of The Potassium-Calcium Distribution Relationship In Predentine, H. J. Höhling, H. Mishima, Y. Kozawa, T. Daimon, R. H. Barckhaus, K. -D. Richter

Scanning Microscopy

Apex regions of continuously growing incisors of Wistar rats were quickly dissected, shock-frozen in liquid nitrogen-cooled propane, freeze-dried at -80 °C and infiltrated with Spurr's resin . 400nm thick dry sections were cut with a diamond knife on an ultramicrotome . Relatively flat sections were transferred with an eye lash onto collodium coated aluminum grids. They were flattened with a glass stick and by placing another collodi um coated aluminum grid just on top of the first one, exerting a uniform pressure . After carbon coating the sections were observed using the backscattered and secondary electron signals in a scanning …

X-Ray Microanalysis Of Ca And K In Corn Bran And Oat Hulls, F. R. Dintzis, F. L. Baker, T. C. Nelsen

Scanning Microscopy

Oat hulls and dry-milled corn bran were loaded with calcium or potassium and made into either sectioned bulk specimens of intact tissue embedded in resin or into non-sectioned bulk specimens made from powdered-compressed tissue formed into disks without resin. Regression lines of X-ray count versus mineral concentration were similar for both Ca and K. X-ray count versus mineral concentration relationships were similar for intact oat hulls and powdered-compressed specimens of either oat hulls or corn bran. However, the relationship for intact corn bran embedded in resin was significantly different. While the reason for this difference is not known, the result …

Studies Of Epithelial Electrolyte Transport By Marker Ions, A. Dörge, R. Rick

Scanning Microscopy

The paper reviews several recent studies in which marker ions, such as Rb and Br, were used to identify ion transport pathways and membrane properties in epithelia. In the frog skin epithelium, using Rb as a substitute for K, Cl transport mechanisms across the basolateral membranes of principal cells were studied. The data suggest that intracellular Cl is maintained above electrochemical equilibrium by an Na-K-2Cl cotransport system which, under non-stimulated conditions, is normally quiescent.

In toad and frog skins, the route of transepithelial Cl movement was investigated. A subpopulation of mitochondria-rich cells demonstrated a ready exchange of Br with the …

Evidence That A Major Portion Of Cellular Potassium Is "Bound", Ivan L. Cameron, W. Elaine Hardman, Keithley E. Hunter, Christine Haskin, Nancy K. R. Smith, Gary D. Fullerton

Scanning Microscopy

In this report we briefly review recent evidence which shows that a substantial proportion of intracellular K⁺ is "bound" or perturbed from the physicochemical properties expected in dilute aqueous solutions. In addition, we present evidence from electron probe x-ray microanalysis of thin cryosections of cells which indicates that the binding of K⁺ to anionic groups either carboxyl groups (HCO₂) on proteins or to phosphate groups in creatine phosphate (CrP) , in adenosine triphosphate, (ATP), in protein and in nucleic acids, are the main determinants of the maintenance of (as differentiated from the generated of) the well …