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Electrochemical Dna-Based Biosensor For The Detection Of Dnase I Activity For Potential Application For Crispr-Cas Based Biosensor, Tessa Siler
Fall Student Research Symposium 2022
Dioxyribonuclease I (DNase I, DC 3.1.21.1) is an enzyme that cleaves DNA at phosphodiester linkages. The deviation of DNase I activity is a significant important marker for several diseases. For example, the lower activity of DNase can cause arthritis and several other autoimmune diseases, whereas higher DNase I activities are observed in patients with breast and oral cavity cancers. This makes monitoring the DNase I activity important for clinical and point-of-care diagnostics.Several methods were developed to assay DNase I activity, such as the enzyme-linked immunosorbent assay (ELISA), fluorimetric assays, radial immunodiffusion (RID) and radial enzyme diffusion (RED) assays. These methods …