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Articles 1 - 11 of 11
Full-Text Articles in Life Sciences
Hepatobiliary Analyses Suggest Chronic Pah Exposure In Hakes (Urophycis Spp.) Following The Deepwater Horizon Oil Spill, Rachel E. Struch, Erin Pulster, Andrea D. Schreier, Steven A. Murawski
Hepatobiliary Analyses Suggest Chronic Pah Exposure In Hakes (Urophycis Spp.) Following The Deepwater Horizon Oil Spill, Rachel E. Struch, Erin Pulster, Andrea D. Schreier, Steven A. Murawski
C-IMAGE Publications
Prior to the Deepwater Horizon oil spill, we lacked a comprehensive baseline of oil contamination in the Gulf of Mexico's sediments, water column, and biota. Gaps in prespill knowledge limit our ability to determine the aftereffects of the Deepwater Horizon blowout or prepare to mitigate similar impacts during future oil spill disasters. We examined spatiotemporal differences in exposure to and metabolism of polycyclic aromatic hydrocarbons (PAHs) in 2 hake species (Urophycis spp.) to establish a current baseline for these ecologically important, abundant, and at-risk demersal fishes. Gulf hake (Urophycis cirrata) and southern hake (Urophycis floridana) were collected throughout the Gulf …
Hepatic Polycyclic Aromatic Hydrocarbons (Pahs) Concentrations In Red Hind (Epinephelus Guttatus) Collected Aboard Multiple R/V Weatherbird Ii Cruises In The Southern Gulf Of Mexico (Mexico And Cuba) From 2015-09-18 To 2017-05-23, Erin Pulster, Steven A. Murawski
Hepatic Polycyclic Aromatic Hydrocarbons (Pahs) Concentrations In Red Hind (Epinephelus Guttatus) Collected Aboard Multiple R/V Weatherbird Ii Cruises In The Southern Gulf Of Mexico (Mexico And Cuba) From 2015-09-18 To 2017-05-23, Erin Pulster, Steven A. Murawski
C-IMAGE data
This dataset contains hepatic polycyclic aromatic hydrocarbons (PAHs) concentrations in Red Hind (Epinephelus guttatus) collected aboard R/V Weatherbird II cruises (WB1603, WB1701, WB1731) in the southern Gulf of Mexico (Mexico and Cuba) from 2015-09-18 to 2017-05-23. The dataset includes the location, date, concentrations of various PAHs in liver tissue (ng/g w.w.), and the total or sum of the low and high molecular weight PAHs (ng/g w.w.). PAHs were extracted from homogenized liver tissues using QuEChERS EMR (Agilent Technologies, Bond Elut). Individual PAHs were confirmed and quantified using an Agilent’s 7890B gas chromatograph (GC) coupled to a 7010 tandem mass spectrometer …
Histological Examination Of Livers Of Golden Tilefish (Lopholatilus Chamaeleonticeps) Collected Aboard Multiple R/V Weatherbird Ii Cruises In The Gulf Of Mexico From 2012-08-14 To 2016-09-06, Erin Pulster, S. Fogelson, S. Synder
Histological Examination Of Livers Of Golden Tilefish (Lopholatilus Chamaeleonticeps) Collected Aboard Multiple R/V Weatherbird Ii Cruises In The Gulf Of Mexico From 2012-08-14 To 2016-09-06, Erin Pulster, S. Fogelson, S. Synder
C-IMAGE data
This dataset contains a histological examination of livers of golden tilefish (Lopholatilus chamaeleonticeps) collected aboard the R/V Weatherbird II cruises WB-1304, WB-1403, WB-1502, WB-1602, WB-1603, and WB-1701 in the Gulf of Mexico from 2012-08-14 to 2016-09-06. The dataset includes the identified presence of histological changes and images of the gross and histological evaluations. Formalin-fixed tissues were individually evaluated for color, texture, size, shape, and consistency. Photographs were taken with a Sony-Cybershot camera (Sony Corporation, Minato, Tokyo, Japan). At least two photographs were taken of the samples from each container. The first photo contained the label along with the tissues from …
Pathological Findings In Red Snapper Livers Collected Aboard Multiple R/V Weatherbird Ii Cruises In The North Central Region, Gulf Of Mexico From 2012-08-13 To 2017-08-01, Erin Pulster, S. Fogelson, Steven A. Murawski
Pathological Findings In Red Snapper Livers Collected Aboard Multiple R/V Weatherbird Ii Cruises In The North Central Region, Gulf Of Mexico From 2012-08-13 To 2017-08-01, Erin Pulster, S. Fogelson, Steven A. Murawski
C-IMAGE data
This dataset contains pathological findings in red snapper (Lutjanus campechanus) livers collected aboard the multiple Weatherbird II cruises WB1304 (2012-08-13 to 2012-08-25), WB1403 (2013-08-15 to 2013-08-30), WB1502 (2014-08-05 to 2014-08-20), WB1602 (2015-08-15 to 2015-08-30), WB1736 (2017-07-18 to 2017-08-01) in the north central region of the Gulf of Mexico, post-Deepwater Horizon. The dataset includes the identified presence of histological changes and images of the gross and histological evaluations. Formalin-fixed tissues were individually evaluated for color, texture, size, shape, and consistency. Photographs were taken with a Sony-Cybershot camera (Sony Corporation, Minato, Tokyo, Japan). At least two photographs were taken of the samples …
Concentrations Of Polycyclic Aromatic Hydrocarbons (Pahs) In Liver Tissue Of Golden Tilefish Collected Aboard Multiple R/V Weatherbird Ii Cruises In The Gulf Of Mexico From 2012-08-13 To 2017-08-01, Erin Pulster, S. M. Synder, Steven A. Murawski
Concentrations Of Polycyclic Aromatic Hydrocarbons (Pahs) In Liver Tissue Of Golden Tilefish Collected Aboard Multiple R/V Weatherbird Ii Cruises In The Gulf Of Mexico From 2012-08-13 To 2017-08-01, Erin Pulster, S. M. Synder, Steven A. Murawski
C-IMAGE data
This dataset contains the concentrations of polycyclic aromatic hydrocarbons (PAHs) in liver tissue of golden tilefish (Lopholatilus chamaeleonticeps) collected aboard multiple R/V Weatherbird II cruises WB1304 (2012-08-13 to 2012-08-25), WB1403 (2013-08-15 to 2013-08-30), WB1502 (2014-08-05 to 2014-08-20), WB1602 (2015-08-15 to 2015-08-30), WB1603 (2015-09-10 to 2015-10-02), WB1701 (2016-08-02 to 2016-09-09), and WB1736 (2017-07-18 to 2017-08-01) in the Gulf of Mexico during the Gulf of Mexico-wide surveys from 2012-2017. PAH was extracted from livers with a modified QuEChERS (Bond Elut, Agilent Technologies, Santa Clara, CA, USA) method optimized specifically for this study species. Extracts were analyzed using Agilent’s 7890B gas chromatograph (GC) …
A Multivariate Approach For An Improved Assessment Of Pre-Erythrocytic Stage Therapies Targeting Plasmodium Vivax And Plasmodium Falciparum, Alison E. Roth
A Multivariate Approach For An Improved Assessment Of Pre-Erythrocytic Stage Therapies Targeting Plasmodium Vivax And Plasmodium Falciparum, Alison E. Roth
USF Tampa Graduate Theses and Dissertations
The malaria pre-erythrocytic stages have been identified as an ideal therapeutic target, but complex in vitro models for Plasmodium vivax and Plasmodium falciparum lack the efficiency needed for rapid screening and evaluation of new vaccines and drugs, especially targeting the P. vivax hypnozoite. To address this challenge, we employed a multi-parameter approach using “omics’” to identify pre-erythrocytic targets and biomarkers, guide phenotypic therapeutic screening, and study parasite functionality with innovative bioassays using highcontent screening. Herein, we discuss three novel bioassays formatted in 384-well plate systems with utilization of commercially-available materials and application of high-content imaging for rapid bio-image analysis. To …
Quantitative Proteomic Profiling Reveals Hepatic Lipogenesis And Liver X Receptor Activation In The Pander Transgenic Model., Mark G. Athanason, Whitney A. Ratliff, Dale Chaput, Catherine B. Marelia, Melanie N. Kuehl, Stanley M. Stevens Jr., Brant R. Burkhardt
Quantitative Proteomic Profiling Reveals Hepatic Lipogenesis And Liver X Receptor Activation In The Pander Transgenic Model., Mark G. Athanason, Whitney A. Ratliff, Dale Chaput, Catherine B. Marelia, Melanie N. Kuehl, Stanley M. Stevens Jr., Brant R. Burkhardt
Molecular Biosciences Faculty Publications
PANcreatic-DERived factor (PANDER) is a member of a superfamily of FAM3 proteins modulating glycemic levels by metabolic regulation of the liver and pancreas. The precise PANDER-induced hepatic signaling mechanism is still being elucidated and has been very complex due to the pleiotropic nature of this novel hormone. Our PANDER transgenic (PANTG) mouse displays a selective hepatic insulin resistant (SHIR) phenotype whereby insulin signaling is blunted yet lipogenesis is increased, a phenomena observed in type 2 diabetes. To examine the complex PANDER-induced mechanism of SHIR, we utilized quantitative mass spectrometry-based proteomic analysis using Stable Isotope Labeling by Amino Acids in Cell …
Antimalarial Exoerythrocytic Stage Drug Discovery And Resistance Studies, Lynn Dong Blake
Antimalarial Exoerythrocytic Stage Drug Discovery And Resistance Studies, Lynn Dong Blake
USF Tampa Graduate Theses and Dissertations
Malaria is a devastating global health issue that affects approximately 200 million people yearly and over half a million deaths are caused by this parasitic protozoan disease. Most commercially available drugs only target the blood stage form of the parasite, but the only way to ensure proper elimination is to treat the exoerythrocytic stages of the parasite development cycle. There is a demand for the discovery of new liver stage antimalarial compounds as there are only two current FDA approved drugs for the treatment of liver stage parasites, one of which fails to eliminate dormant forms and the other inducing …
Hepatic Nutrient And Hormonal Regulation Of The Pancreatic-Derived Factor (Pander) Promoter, Whitney Ratliff
Hepatic Nutrient And Hormonal Regulation Of The Pancreatic-Derived Factor (Pander) Promoter, Whitney Ratliff
USF Tampa Graduate Theses and Dissertations
PANcreatic-DERived factor (PANDER, FAM3B) has been shown to regulate glycemic levels via interactions with both pancreatic islets and the liver. Although PANDER is predominantly expressed from the endocrine pancreas, recent work has provided sufficient evidence that the liver may also be an additional tissue source of PANDER production. At physiological levels, PANDER is capable of disrupting insulin signaling and promoting increased hepatic glucose production. As shown in some animal models, strong expression of PANDER, induced by viral delivery within the liver, induces hepatic steatosis. However, no studies to date have explicitly characterized the transcriptional regulation of PANDER from the liver. …
Development Of An Efficient Human Hepatocyte Culture Platform For Assessing Novel Therapeutic Efficacy Against Plasmodium Liver Parasites, Steven Patrick Maher
Development Of An Efficient Human Hepatocyte Culture Platform For Assessing Novel Therapeutic Efficacy Against Plasmodium Liver Parasites, Steven Patrick Maher
USF Tampa Graduate Theses and Dissertations
Malaria is a critical and global public health problem, affecting over 200 million people every year, resulting in over 500,000 deaths. A vaccine is not currently available and only one drug, primaquine, is effective against the dormant stages of Plasmodium vivax. Preclinical assessment of novel therapeutic drugs and vaccines is hampered by the lack of an in vitro liver model for P. falciparum and P. vivax. To provide a stable human hepatocyte-based culture platform for parasite development, we engineered a microfluidic bilayer device capable of both simple and complex culture methods, including perfusion and co-culture, to better understand the requirements …
Phenotypic Characterization Of The Pancreatic-Derived Factor (Pander) Knockout Mouse On Pure C57bl/6 Background, Shari Moak
USF Tampa Graduate Theses and Dissertations
PANcreatic-DERived Factor (PANDER), or FAM3B, is a 235-amino acid protein strongly expressed within and secreted from the endocrine pancreas. Research surrounding PANDER has revealed a large role for the protein in maintaining glucose homeostasis, as evidenced by several Ad-PANDER overexpressing murine models, our lab's pancreas-specific PANDER transgenic overexpressor, and most recently our mixed genetic C57/129J PANDER knockout (PANKO) mouse. However, PANDER's overall role in glycemic regulation and glucose homeostasis has yet to be studied in a purebred C57BL/6J PANDER knockout model. Here we present the first phenotypic characterization of our global PANDER knockout mouse on a C57BL/6J background (PANKO-C57) where …