Life Sciences Commons^™

Differential Cholinergic Modulation Of Prelimbic And Thalamic Input To The Basolateral Amygdala, Sarah Catherine Tryon

Theses and Dissertations

The basolateral amygdala (BL) is critical for emotional memory acquisition and expression. It receives afferent projections from both cortical and subcortical regions that send glutamatergic transmission to the BL. This input conveys information necessary for survival, including information about one’s behavioral state and environment. How this information is integrated and processed by the BL, however, remains largely unknown. Interestingly, the BL receives the densest amount of cholinergic innervation from the basal forebrain. This acetylcholine (ACh) can modulate emotional memories, but how it modulates specific afferent inputs to the BL is unexplored. To answer this question, we used brain slice field …

Distribution And Dispersal Potential Of The Endemic Tree Loblolly Bay (Gordonia Lasianthus) In The Southeastern United States Coastal Plain And Implications For Conservation In The Context Of Forecast Climate Scenarios., Herrick Hermann Klaus Brown

Theses and Dissertations

Plant communities in the Southeastern United States Coastal Plain constitute a rich assemblage of species from neighboring floristic provinces and include over 1,000 species endemic to the region. Conservation of these unique landscapes requires a better understanding of potential impacts from human activity and a changing climate. As a model species, the endemic tree Loblolly Bay (Gordonia lasianthus) is representative of Carolina Bays, pocosins, and isolated wetland habitats that accentuate Longleaf Pine ecosystems which dominate the focus area. Seed-dispersal events in Loblolly Bay are limited to periods of relatively low humidity primarily during the months of October to December, and …

Study Of The Inflammatory And Fibrotic Pathology In The Gut Following Microcystin Exposure Under Conditions Of Non-Alcoholic Fatty Liver Disease., Sutapa Sarkar

Theses and Dissertations

Non-alcoholic fatty liver disease (NAFLD) has become a global pandemic with increased risks of chronic inflammation in obese and elderly population. It has become a major public health concern due to the unavailability of proper therapeutic approaches. NAFLD is a condition of the liver involving wide spectrum of events including accumulation of fat in the liver and inflammation, which can progress to a fibrotic and cirrhotic phenotype, often termed as non-alcoholic steatohepatitis (NASH). In this backdrop, exposure to environmental toxins from harmful algal blooms could prove detrimental to the overweight, obese or elderly population. NAFLD not only affects the liver …

Salt Marsh Health And Biomass Responses To A Changing Environment, Gwen Joelle Miller

Theses and Dissertations

Coastal salt marshes are important ecosystems not only for their aesthetic beauty but also for their ecosystem services that they provide including improving water quality, providing protection from storm surges and hurricanes, and carbon sequestration. With climate change, including drought, warmer temperatures and sea-level rise, these systems are going to be impacted. Understanding how salt marshes will respond, or already have responded, to climate change will help us be better prepared for the future. By scripting a model to project how marshes may migrate with sea-level rise, I discover that salt marshes within Beaufort and Jasper counties, South Carolina will …

Distribution And Abundance Of Vibrio Parahaemolyticus In Surficial Estuarine Sediment From North Inlet, Sc, Usa, India Briana Gartmon

Theses and Dissertations

Vibrio parahaemolyticus is a ubiquitous coastal organism that causes gastroenteritis (vibriosis) after ingestion of mishandled or undercooked seafood. Cases of vibriosis have increased rapidly in the past decade. V. parahaemolyticus densities have historically been associated with an increase in sea surface water temperatures, but recent shellfish bed closures in fall and winter months in cooler regions (the Baltic Sea, the Pacific NW, and New England) suggest that there may be additional environmental parameters that affect densities of V. parahaemolyticus. The vertical distribution of V. parahaemolyticus was assessed in the pristine North Inlet Estuary (SC, USA). The goal of this …

Revisiting The Standard Soil Test: The Potential Of Macro- And Microbiological Measures To Improve Agricultural Health And Productivity, Gabriel Jacob Kenne

Theses and Dissertations

The agricultural ecosystem has become the largest non-ice-covered terrestrial ecosystem on the planet and has significant direct and indirect impacts on the global health of both the environment and humanity. Recent shifts in modern agriculture are beginning to focus on the health of soil versus a focus traditionally on crop health and yield at any cost. By managing farmland regeneratively, systems are able to reverse many of the problems created by conventional land management such as erosion and unwanted nutrient runoff, and simultaneously create opportunities to move C from the atmosphere back into the soil. While the ways in which …

Larval Fish Mortality And Vertical Chlorophyll Structures: Reexamination Of The Stable Ocean Hypothesis In The Southern California Current, Brendan D. Turley

Theses and Dissertations

The natural mortality of fishes is an important component for understanding population dynamics. The larval stages of pelagic fishes living in the open ocean are particularly vulnerable to high rates of mortality, and fluctuations in these rates are thought to exert a large influence on the number of fish maturing into the spawning population. Early stage larval fishes are thought to undergo a critical period after hatching when they must find food or succumb to starvation. While the availability of suitable food for larval fishes is, on average, too low in the open ocean to support survival, patchy distributions of …

Chronic Kidney Disease From Non-Traditional Causes Throughout Central America, Abigail K. Watson

Senior Theses

Throughout many Central American countries, incidence of chronic kidney disease (CKD) has been on the rise. The disease mainly affects agricultural workers and differs from typical CKD. Patients in these countries often do not have preexisting conditions such as diabetes or hypertension known to be traditional causes of CKD. They also experience increased damage to the kidney tubules, rather than the glomeruli generally more heavily impacted. There has been speculation regarding the causes of CKDnT (chronic kidney disease of nontraditional causes), but no consensus has been reached. Two major hypotheses to explain the high prevalence among Central American sugarcane workers …

Microcystin Exposure And Liver Injury Outcomes In Nafld, Muayad Albadrani

Theses and Dissertations

Non-alcoholic fatty liver disease (NAFLD) is an emerging worldwide pandemic which is highly prevalent among obese individuals including children and adults. In a NAFLD condition, exposure to environmental contaminants or toxins can act as a second/ multiple hit, which leads to the progression of simple steatosis to NASH and ultimately may result in liver cirrhosis. With climate change today, elevated levels of microcystins are an emerging problem in fresh water bodies, which are a source of drinking water. Individuals are therefore at risk of exposure to microcystin through consuming contaminated water. In the progression of NAFLD from one clinical stage …

Reproducibility And The Effects Of Exercise On The Endurance Index, Michael Dean Smith, Jr.

Theses and Dissertations

Background: The Endurance Index (EI%) is a method to objectively assess a muscle’s ability to resist fatigue. The purpose of these studies was to determine the reproducibility of the EI% and determine the influence of muscular fatigue on the EI%. Methods Study 1: sixteen apparently healthy participants (18-30yrs) completed three nonconsecutive visits each with 3 bouts of 5-minute electrical muscle stimulations (EMS) and accelerometer-based mechanomyography (aMMG) of the vastus lateralis at 4 Hz and low amperage (25-35mA) on the hamstrings of the dominant leg; peak torque was measured at each visit. Study 2: fifteen apparently healthy participants (ages 18-30yrs) …

Evidence For P53-Mediated Induction Of Wrap53a In Response To Dna Damage, Anne Shelton Hucks

Theses and Dissertations

p53 is a powerful tumor suppressor mutated in approximately half of all cancers. Its mRNA is stabilized post-transcriptionally via complementary base pairing with the transcript of its antisense gene, WRAP53α; without this interaction, p53 protein cannot accumulate enough to carry out its many functions related to apoptosis, cell cycle arrest, and DNA damage repair. Previous studies have shown that WRAP53α is induced in response to DNA damage. The purpose of this study was to determine which transcription factors might be responsible for this induction. After identifying three putative p53 binding sites on the WRAP53α promoter, we used chromatin immunoprecipitation …

Assessing The Competitive Advantage Of Carbonic Anhydrase In Estuarine Microalgae Through Removed Enzymatic Activity, Eilea R. Knotts

Theses and Dissertations

Carbon concentrating mechanisms (CCMs) are used by photoautotrophs to overcome possible limitations in carbon acquisition but the competitive strategies and efficiencies of these mechanisms among photosynthesizers can be variable. The diversity in carbon acquisition abilities establishes the potential for alterations in community structure with shifting carbon concentrations. Given the role of phytoplankton and benthic microalgae (BMA) in the trophodynamics of estuaries, understanding the mechanisms of carbon acquisition in these systems is important in predicting how primary productivity and nutrient cycling might change in response to increasing concentrations of atmospheric CO2. Our approach to investigate whether induced carbon limitation would show …

Investigating The Relationship Of Copd, Lung Cancer, And Polycyclic Aromatic Hydrocarbons From Ambient Air Pollution, Daniel John Kilpatrick

Theses and Dissertations

Polycylic aromatic hydrocarbons (PAHs) are a group of chemically-related compounds with a well-established body of evidence of adverse human health impacts. Additionally, there is a growing area of research on the health outcomes following PAH exposure via air pollution, specifically with chronic obstructive pulmonary disorder (COPD) and lung cancer. The purpose of this dissertation was to investigate the strength of associations among PAHs, COPD, and lung cancer from ambient (outdoor) air pollution. The first objective was to determine if there is a potential environmental link among PAHs, COPD, and lung cancer occurrence among nonsmokers using data from the National Health …

Population And Kin Structure Of Hawksbill Turtles: Insights On Natal Homing Precision, Time To Maturity And The Male Component Of The Breeding Population, Kathryn Erin Levasseur

Theses and Dissertations

Marine turtles have long endured population declines and face a growing number of contemporary threats, highlighting the need for population assessments and conservation action. Research on these species, however, remains a challenge due to complex and extensive oceanic life cycles that hinder direct observation. The pelagic, post-hatchling life stage is particularly difficult to track, preventing empirical research of fundamental behavior and life history traits such as natal homing precision and time to sexual maturity. Also, much of our current knowledge of marine turtles comes from nesting females and hatchlings, stages of the life cycle that are easy to observe. Far …

The Effect Of Patient Financial Liability On Physical Therapy Utilization And Patient Reported Outcomes For Patients With Low Back Pain: An Instrumental Variable Analysis, Adam D. Lutz

Theses and Dissertations

Low back pain (LBP) is the most common orthopedic complaint managed by physical therapists in the United States. Insurance plans that require greater patient financial liability per physical therapy visit are thought to disincentivize participation in recommended physical therapy plans of care. The primary purpose of this investigation was to use instrumental variable regression to investigate the effect of insurancemediated patient financial liability per visit on physical therapy visit count and functional patient reported outcomes. The secondary purpose of the study was to examine the generalizability of results to the broader excluded patient population—and beyond to patients that seek physical …

The Role Of Ampk In The Regulation Of Skeletal Muscle Proteostasis During Cancer Cachexia, Dennis K. Fix

Theses and Dissertations

Cancer-induced cachexia is a debilitating disease that diminishes quality of life due to severe loss of skeletal muscle mass and function. Skeletal muscle proteostasis fluctuates diurnally depending on different fasting and feeding durations as well as in response to activity and exercise stimuli. Nutrient availability is key in the net loss and gain of skeletal muscle protein. The adenosine monophosphate protein kinase (AMPK) is a nutrient sensitive kinase that has long been established as a critical regulator of both protein synthesis and protein degradation. AMPK is capable of being activated by many different stimuli as well as diseases such as …

Effects Of Spinal Manipulation On Brain Activation In Individuals With Chronic Low Back Pain, Max K. Jordon

Theses and Dissertations

Chronic low back pain (cLBP) continues to be one of the most common health conditions in the United States. Despite an enormous amount of research, there are no treatments for this condition that consistently improve outcomes. For decades health professionals have incorporated spinal manipulative therapy (SMT) into their practice, but the evidence to date has shown that SMT has only small to modest effect sizes when treating cLBP. One way to improve the effectiveness of SMT is by getting a better understanding of its underlying mechanisms so that the intervention be more specifically targeted to the appropriate individual.

While biomechanical …

Regulation Of Arabidopsis Floral Organ Development By The Transcription Factor Aintegumenta-Like6, Jorman Heflin

Theses and Dissertations

Flowers, and the fruits and seeds they produce, are of great agricultural and economic importance. Understanding the molecular mechanisms that mediate the identity, size and arrangement of floral organs within the flower may allow us to tune these elements for increased crop productivity. I examine several potential regulatory targets of the Arabidopsis AINTEGUMENTA-LIKE6 (AIL6) transcription factor that is involved in these processes and I identify protein-protein interactions that may mediate its activity in flowers. As described by the ABCE model, unique floral organ identities are specified in each whorl of the flower by different combinations of homeotic gene activities. Two …

The Effects Of Climate Change On The Ecotoxicology Of Contaminants Of Emerging Concern: Flame Retardants, Contemporary Use Pesticides And Pharmaceuticals Personal Care Products On The Estuarine Grass Shrimp, Palaemontes Pugio, Rajaa Nouri Al-Yassein

Theses and Dissertations

Global Climate Change may adversely affect the environment, increasing water temperature and altered salinity which may affect the toxicity of both legacy pollutants and Contaminants of Emerging Concern (CECs). Acute, 96 hour toxicity tests with adult grass shrimp (Palaemonetes pugio) assessed the effects of CECs (Polybrominated Diphenyl Esther (PBDE) – 47, ibuprofen, bifenthrin, triclosan, and bifenthrin/triclosan mixtures) under Standard Conditions (20°C, 20psu) and different Climate Change Conditions (30°C, and/or35psu) In addition, the grass shrimp microbiome (e.g. Vibrio bacteria) were assessed following acute triclosan exposures at the Maximum Exposure Concentration (MECs) and Minimum Inhibitory Concentration (MICs) under standard conditions. …

Leveraging For-Cause Physical Activity Events For Physical Activity Promotion: An Investigation Using Self-Determination Theory, John A. Bernhart

Theses and Dissertations

Motivating individuals to live active lifestyles remains a challenging but important public health issue. For-cause physical activity events reach large groups of people, many of whom are not regularly active. However, little research has applied established health behavior theories to explain participation in for-cause events. Therefore, the purpose of this dissertation was to investigate participation in for-cause events through the lens of Self-Determination Theory (SDT).

The first study recruited participants (n=207) registered in a for-cause PA event (i.e., 5K distance or shorter) to complete online surveys that assessed need satisfaction for autonomy, competence, and relatedness; intrinsic motivation; altruism; PA behavior; …

The Persistent Maladaptation Of Pieris Macdunnoughii: Constraints On Adaptation To An Evolutionary Trap, Rachel A. Steward

Theses and Dissertations

Evolutionary traps arise when organisms use novel, low-quality or even lethal resources based on previously reliable cues. Persistence of such maladaptive interactions depends not only on how individuals locate important resources, such as host plants, but also the mechanisms underlying poor performance. Pieris macdunnoughii (Remington) (Lepidoptera: Pieridae) lays eggs on a non-native mustard, Thlaspi arvense (L.) (Brassicaceae), which is lethal to its larvae. However, in the 150 years since T. arvense invaded this butterfly continues to recognize and oviposit on the invasive mustard.

I evaluated two possible constraints on the evolution of decreased preference within an invaded population. First, an …

The Role Of The Gut Microbiome In Tumor Development And Response To 5-Fluorouracil In The Apc^Min/+ Mouse, Kristen Michele Hogan

Theses and Dissertations

Colorectal cancer (CRC) is the third most frequent cancer and the second leading cause of cancer related deaths. While CRC is initiated by mutations in critical tumor suppressor genes, its development and progression to malignancy is aided by host derived cells that are recruited to the tumor microenvironment (TME) and by cancer-associated microbiota that constitute the gut microbiome. Components of the gut microbiota have been shown to alter immune cell infiltration into tumors and influence response to therapy, disease stage, and patient survival. In previous studies, we found that ApcMin/+ mice, a model of early colon carcinogenesis, had reduced tumor …

The Health Implication And Application Of Silver And Iron Oxide Nanoparticles, Sahar Pourhoseini

Theses and Dissertations

Nanoscience and nanotechnology have successfully created different nanomaterials, which are defined as materials with at least one dimension between 1-100 nm. This small size gives nanomaterials unique and novel properties compared with bulk material, such as a high surface area, specific mechanical, optical and magnetic properties. These novel properties have stimulated the interest of using nanoparticles in a wide range of consumer products and applications including biomedical, pharmaceutical and drug delivery. Silver and iron oxide nanoparticles attracted more attention commercially due to their antibacterial and magnetic properties, respectively. So, the overall objective of this dissertation was: 1) to examine the …

Proteomic And Genomic Evolution Of Phicbkviruses, Kiesha Wilson

Theses and Dissertations

It is estimated that there are 10³¹ phages in the global population. They continue to maintain their populations by undergoing 10²⁴ infections per second. Every time these phages replicate, there is potential for genetic exchange and mutations. Because of this potential, it has been assumed that phage evolution is heavily influenced by mosaicism. However, it is now clear that phage evolution varies by host type and phage type. Here, we use genomic and proteomic analyses to study the evolution of phages that infect Caulobacter crescentus. The study began by comparing six bacteriophage genomes that were larger than …

Energetic Consequences Of Human Impacts For Bioindicator Atlantic Ghost Crab (Ocypode Quadrata), Mustafa Remzi Gül

Theses and Dissertations

Human activities have caused notable changes in ecosystems globally over the past century, which cause substantial ecological issues. The degree of these ecological issues is often investigated using the responses of bioindicator species. Bioindicator species often reduce their population densities and individual sizes as a response to the human disturbance. Observing these variations in the demographics is often an effective way to measure the strength of the ecological changes. However, they do not provide any mechanistic reason (s) for the shifts in the demographics, which is important for making predictions about responses of the species to the future conditions. Therefore, …

Physical Activity Of Preschoolers With Developmental Disabilities And Delays, Michaela A. Schenkelberg

Theses and Dissertations

Children with developmental disabilities and delays are at greater risk for developing overweight and obesity compared to typically developing peers. Participation in regular physical activity is a modifiable behavior that is consistently associated with improved weight status and other positive health outcomes. Previous studies have identified numerous individual- and environmental-level factors that associate with physical activity among school-age children with and without disabilities. However, little is known about physical activity behaviors and related correlates among preschool-aged children with disabilities (ages 3 – 5 years), especially while they are in preschool settings. Therefore, the overall purpose of this dissertation was to …

Cellular Localization Of Rad51d Mutant Proteins And The Application Of Art To Increase Scientific Literacy In America, Claire L. Chabot

Senior Theses

Ovarian cancers are the leading cause of death from cancer of the female reproductive system. Approximately 50% of ovarian cancers have defects in the homologous recombination (HR) DNA repair pathway that is required for the repair of DNA double-stranded breaks. The status of HR genes, such as BRCA1, BRCA2, and the RAD51 family, contributes to ovarian cancer development as well as treatment decisions regarding chemotherapy, radiation, and immunotherapy. The overarching goal of this project is to identify new insights into HR that can integrate with Precision Medicine Initiatives and align with the goals of the Cancer Moonshot 2020 Program. I …

Synthetic Lectins, A Diagnostic And Prognostic Tool For Detecting Glycans In Breast Cancer, Daniel James Gordon

Theses and Dissertations

Cancer diagnostic tools have been pushed to the forefront of medical research because a person’s chance of survival is directly correlated with how early the tumor is identified and treatment is begun. In searching for the subtle differences between healthy and tumor cells, almost every type of cancer has been shown to under, over, or neo express glycans, and these changes in the glycan fingerprint can continue as the disease progresses. This provides a powerful diagnostic opportunity that’s works by screening for glycoproteins and glycosylation patterns that deviate from normal cells.

Boronic acids have a useful and tunable property in …

The Perspectives Of Emerging Adults With Hereditary Diffuse Gastric Cancer, Carrie Anderson

Theses and Dissertations

Individuals with hereditary diffuse gastric cancer (HDGC) caused by a CDH1 mutation have a high lifetime risk of developing gastric cancer. National guidelines recommend CDH1 carriers undergo a prophylactic total gastrectomy (PTG)— a surgery that greatly reduces the risk of developing gastric cancer but has significant comorbidities. This study explores the impact of a diagnosis of HDGC in the transitional life stage between the ages of 18-29 deemed “emerging adulthood.” We surveyed 21 CDH1 carriers and conducted semi-structured phone interviews with 6 CDH1 carriers between the ages of 18-29 to learn about their life experiences regarding education, career, relationships, and …

Exploring Patient Perceptions And Misconceptions: Beliefs Regarding Hereditary Cancer, Margaret Flach

Theses and Dissertations

Many patients who enter a genetic counseling session have preconceived notions about why they or their family members developed a genetic condition. Often these perceptions are deeply rooted in personal, familial, and/ or cultural beliefs; individuals typically have a personal framework, or schema, into which they incorporate new information. There is limited research on what information patients are retaining during a genetic counseling session and how they are assimilating that knowledge into their existing views. We attempted to characterize these patient perceptions with respect to hereditary cancer, in order to assess how patients are adopting the information presented in a …