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Articles 1 - 30 of 36
Full-Text Articles in Life Sciences
The Effects Of The Ground Reaction Force On The Muscle-Tendon Systems About Guinea Pig Joints, Theresa Schill, Kevin Mailland, David V. Lee
The Effects Of The Ground Reaction Force On The Muscle-Tendon Systems About Guinea Pig Joints, Theresa Schill, Kevin Mailland, David V. Lee
Undergraduate Research Opportunities Program (UROP)
Each limb of the limb joints of a quadruped is used differently during locomotion, and it is necessary to recognize the mechanical role each plays. The effects of the ground reaction force on the ankle, knee, and hip joints of Hartley albino guinea pigs were studied in uphill, downhill, and level situations to determine the spring-like capacity of the muscle-tendon systems that act about the limb joints.
Similarity Of Climate Change Data For Antarctica And Nevada, Corbin Benally, Shahram Latifi, Karletta Chief
Similarity Of Climate Change Data For Antarctica And Nevada, Corbin Benally, Shahram Latifi, Karletta Chief
Undergraduate Research Opportunities Program (UROP)
The correlation between temperature and carbon dioxide concentration in the past one hundred years is studied. Separate graphs containing data from Vostok, Antarctica and the Mojave desert/mountain west (Nevada region) are presented. Using data obtained from these graphs, an attempt is made to explain the results and investigate the similarity of these results for Antarctica and Nevada. The importance of this study lies in the fact that if data show the same trend in the two regions, many findings for climate change in Antarctica may readily be validated and employed for Nevada.
Construction And Use Of A Calorimeter To Estimate The Anaerobic Contributions To Metabolism, Mark S. Burger, Frank Van Breukelen
Construction And Use Of A Calorimeter To Estimate The Anaerobic Contributions To Metabolism, Mark S. Burger, Frank Van Breukelen
Undergraduate Research Opportunities Program (UROP)
Metabolism is derived from the Greek word metabole which translates to change. Metabolism is the sum total of chemical reactions that take place in an organism. Some reactions are exothermic (heat-producing) whereas others are endothermic (heat-absorbing). By measuring heat production (calorimetry), one can estimate metabolic rate. Historically, such measurements of direct calorimetry were difficult. As a result, most studies utilize indirect calorimetry wherein oxygen consumption and/or carbon dioxide production are measured. One limitation to this approach is that anaerobic metabolism is ignored. No commercially-available calorimeter is available for whole animal metabolic studies. We hypothesized that small rodent hibernators may experience …
Antibiotic Resistance In Bacillus Subtilis As Affected By Transcriptional Derepression And The Stringent Response, Brandon Eisinger, Ronald E. Yasbin, Eduardo A. Robleto
Antibiotic Resistance In Bacillus Subtilis As Affected By Transcriptional Derepression And The Stringent Response, Brandon Eisinger, Ronald E. Yasbin, Eduardo A. Robleto
Undergraduate Research Opportunities Program (UROP)
Bacterial cells under conditions of starvation or prolonged non-lethal selective pressures accumulate mutations in highly transcribed genes. This process is part of cellular programs to increase genetic diversity in conditions of stress, also known as stationary phase or stress-induced mutagenesis. This experiment investigated mutation frequencies for antibiotic resistance as affected by the stringent response. The stringent response is a global cellular process that initiates at the cessation of growth and mediates changes in gene expression that repress synthesis of ribosome components. We used Bacillus subtilis strains that differ in RelA proficiency. The relA gene controls the synthesis of (p)ppGpp, the …
Impact Of Historical Climate Change On The Genetic Structure Of The Great Basin Pocket Mouse, Perognathus Parvus, Ashwitha Francis, Mallory E. Eckstut, Brett R. Riddle
Impact Of Historical Climate Change On The Genetic Structure Of The Great Basin Pocket Mouse, Perognathus Parvus, Ashwitha Francis, Mallory E. Eckstut, Brett R. Riddle
Undergraduate Research Opportunities Program (UROP)
The dynamics of genetic patterns, such as genetic differentiation within and between populations and the geographic distribution of genetic lineages, are often influenced by historical events (such as climatic changes) that have substantially impacted regional biodiversity (the study of phylogeography; Avise, 20001). The magnitude of genetic differentiation among populations is often increased if suitable habitat is reduced, because there is restricted migration among populations. When migration is restricted, the populations may become genetically differentiated from conspecific populations and, over time, may become independent lineages that follow distinctive evolutionary trajectories (Stebbins, 19523; Nevo & Beiles, 19892; Thomas et al., 19984). In …
Evaluating Snow Microbial Assemblages, Jenny Lam, Jessica K. Guy, Ryan Brock, Matt Oates, Alison E. Murray
Evaluating Snow Microbial Assemblages, Jenny Lam, Jessica K. Guy, Ryan Brock, Matt Oates, Alison E. Murray
Undergraduate Research Opportunities Program (UROP)
Psychrophiles are organisms that grow optimally below 20C (1). The US Great Basin is home to many mountain peaks with an abundance of alpine snow environments perfect for psychrophilic habitation. We analyzed samples from three different locations, Wheeler Peak, Pacific Crest Trail, and Mount Conness, characterizing and comparing the psychrophilic communities at varying depth intervals in the snow. Polymerase chain reaction (PCR) and denaturing gradient gel electrophoresis (DGGE) showed no notable difference in community structure with depth, but there was a distinct difference when comparing different snow environments (i.e. shaded vs. full sun exposure). The chlorophyll concentration decreased as the …
3d Micro-Mr And Micro-Ct Models For Determining In-Vivo Function In The Guinea Pig Knee Model Of Osteoarthritis (Oa), Kristoffer W. Dean
3d Micro-Mr And Micro-Ct Models For Determining In-Vivo Function In The Guinea Pig Knee Model Of Osteoarthritis (Oa), Kristoffer W. Dean
Undergraduate Research Opportunities Program (UROP)
Osteoarthritis (OA) is a debilitating disease that affects an estimated 27 million adults in the United States. OA causes damage to cartilage in the joints resulting in bones that rub together. This creates pain, swelling, loss of motion of the joint, and bone spurs. As the number of adults above 65 grows (an estimated 72 million people by 2030) even more people will be at high risk for OA. It is the most common type of arthritis by far yet very little is known about it including relationships between joint structure and function. In 2004, the NIH created the Osteoarthritis …
Modifying The Amino Acid Sequence In The Surface-Exposed Loops Of The Omptin Family Of Proteins To Determine Their Effect On Function, Natiera Magnuson, Eun-Hae Kim, Christian Ross, Helen J. Wing
Modifying The Amino Acid Sequence In The Surface-Exposed Loops Of The Omptin Family Of Proteins To Determine Their Effect On Function, Natiera Magnuson, Eun-Hae Kim, Christian Ross, Helen J. Wing
Undergraduate Research Opportunities Program (UROP)
The omptin family of proteins consists of proteases which lie in the outer membrane of some gram-negative, pathogenic bacteria such as Escherichia coli (OmpT), Shigella flexneri (IcsP), Salmonella typhimurium (PgtE), and Yersinia pestis (Pla). These proteases are highly conserved, sharing approximately 50% sequence identity and a β-barrel shape (fig. 1D). The differences in the structure of these four proteins are in the surface-exposed loop region surrounding the active site, but not in the active site itself [4]. These proteases are important for the virulence of many bacteria. For example, OmpT of E. coli cleaves an antimicrobial peptide secreted by epithelial …
Phylogenetic Studies Of Newly Isolated Freshwater Magnetospirilla Using Cbb And Mam Genes, Nathan Viloria, Christopher T. Lefevre, Dennis A. Bazylinski
Phylogenetic Studies Of Newly Isolated Freshwater Magnetospirilla Using Cbb And Mam Genes, Nathan Viloria, Christopher T. Lefevre, Dennis A. Bazylinski
Undergraduate Research Opportunities Program (UROP)
The phylogeny and general relatedness of prokaryotes is determined by comparisons of the sequences of rRNA genes, most commonly the 16S rRNA gene. Comparisons between other gene sequences have been used for this purpose and some have supported conclusions from 16S rRNA genes while others have not. In this study, 13 new magnetospirilla were phylogenetically characterized using the sequences of the 16S rRNA gene as well as the genes for forms I and II ribulose-1,5-bisphosphate carboxylase/oxygenase (RubisCO) (cbbL and cbbM, respectively) and for two magnetosome membrane proteins unique to magnetotactic bacteria, mamJ and mamK. Polymerase chain reaction (PCR) with degenerate …
Genetically Modifying Arabidopsis Thaliana With A Gene From Drought-Tolerant Xerophyte Larrea Tridentata (Creosote Bush), Norris Lam, Liyuan A. Zhang, Lingkun Gu, Qingxi J. Shen
Genetically Modifying Arabidopsis Thaliana With A Gene From Drought-Tolerant Xerophyte Larrea Tridentata (Creosote Bush), Norris Lam, Liyuan A. Zhang, Lingkun Gu, Qingxi J. Shen
Undergraduate Research Opportunities Program (UROP)
L. tridentata, or desert creosote bush, is a xerophytic C3 plant native to the American Southwest, and is known to have evolutionarily developed sophisticated cellular mechanisms to deal with periods of intense abiotic stress. Particularly, complex signaling pathways in L. tridentata allow it to survive in periods of severe water deficiency. Through the findings of Zou et al. [5,6], LtWRKY21 synergistically works with abscisic acid (ABA) to transactivate both ABA-inducible HVA1 and HVA22 promoters. In addition, as ABA and gibberellic acid (GA) pathways are known to act antagonistically. Expectantly, the findings of Zou et al. suggest that LtWRKY21 activates ABA …
Examination Of Germination Receptors Of B. Subtilis And B. Megaterium, Shruti Patel, Alessio Luinetti, Ernesto Abel-Santos, Eduardo Robleto
Examination Of Germination Receptors Of B. Subtilis And B. Megaterium, Shruti Patel, Alessio Luinetti, Ernesto Abel-Santos, Eduardo Robleto
Undergraduate Research Opportunities Program (UROP)
Many bacterial species including those in the Bacilli group form spores as a mechanism to survive harsh conditions such as extreme temperature, radiation, chemicals, and nutrient starvation. By forming spores, they can remain metabolically dormant for an extended period and revert to their vegetative form when environment becomes favorable. This resumption of metabolism and growth is marked by a process called germination that is triggered by exogenous nutrients such as amino acids, sugars, and nucleotides. The (Ger) germination receptors that are postulated to respond to these germinants, in the case of B. subtilis and B. megaterium, are a complex of …
The Small Regulatory Rna Ryhb Regulates Icsa Expression In Shigella Flexneri, Nick Egan, Helen J. Wing
The Small Regulatory Rna Ryhb Regulates Icsa Expression In Shigella Flexneri, Nick Egan, Helen J. Wing
Undergraduate Research Opportunities Program (UROP)
Shigella flexneri is a gram negative non-motile, non-spore forming, rod-shaped bacterium responsible for bacillary dysentery in humans. The master regulator, VirF, initiates a cascade of virulence gene activation by acting as a transcription factor for the gene encoding the global regulator, VirB (1). Production of VirB is also negatively regulated by the regulatory small RNA (sRNA), RyhB 2). Regulatory sRNAs are untranslated RNA molecules involved in the regulation of both transcription and translation. RyhB, a 90 nt sRNA, was first identified in E. coli and subsequently found in all Shigella species. In Shigella this sRNA is maximally expressed in response …
The Endosymbiotic Relationship Of Leguminosae (Fabaceae) And Rhizobium, Sabrina Mcgee, Dolores Maceda, Maria Bustos, Maryknoll Palisoc, Rachel Dahl
The Endosymbiotic Relationship Of Leguminosae (Fabaceae) And Rhizobium, Sabrina Mcgee, Dolores Maceda, Maria Bustos, Maryknoll Palisoc, Rachel Dahl
Undergraduate Research Opportunities Program (UROP)
This project will look at The Endosymbiotic Relationship of Leguminosae (Fabaceae) and Rizobioum. Endoysmbiosis Theory is the concept that mitochondria and chloroplasts are the result of years of evolution initiated by the endocytosis, (the process by which cells absorb material through their cell membrane) of bacteria and algae which, instead of becoming digested, became symbiotic.The mitochondria of eukaryotes evolved from aerobic bacteria, probably rickettsias, living within their host cell and the chloroplasts from cyanobacteria, also known as blue-green algae. Endoysmbiosis is a type of symbiosis in which one organism lives inside the body of another and both function as a …
Evaluation Of Virb Binding Site Contribution To The Regulation Of The Icsp Promoter In Shigella Flexneri, Juan C. Duhart, Maria I. Castellanos, Helen J. Wing
Evaluation Of Virb Binding Site Contribution To The Regulation Of The Icsp Promoter In Shigella Flexneri, Juan C. Duhart, Maria I. Castellanos, Helen J. Wing
Undergraduate Research Opportunities Program (UROP)
Shigella species are gram-negative, rod-shaped bacteria that are closely related to Escherichia coli. Virulent Shigella spp. are intracellular pathogens that invade, replicate and spread through epithelial cells of the lower intestine and cause bacillary dysentery in humans. This disease is characterized by a robust inflammatory response that results in fever, abdominal pain, and bloody diarrhea (3). According to the CDC, approximately 14,000 cases are reported each year in the United States alone. This number however, does not reflect the actual incidence of this disease as many cases go unreported. The molecular pathogenesis of these bacteria lies in the large virulence …
Ubiquitin Dependent Proteolysis In The Frozen Wood Frog, Michael Ulrich, Ken Storey, Frank Van Breukelen
Ubiquitin Dependent Proteolysis In The Frozen Wood Frog, Michael Ulrich, Ken Storey, Frank Van Breukelen
Undergraduate Research Opportunities Program (UROP)
Wood frogs (Rana sylvatica) freeze during the winter. One might presume that homeostatic processes like protein synthesis and degradation cease during freezing. However, few data are available. In collaboration with Dr. Ken Storey (Carelton University, Canada), we are investigating the primary mode of proteolysis in wood frogs representing different points of the freeze-thaw cycle.
Effects Of Starvation Selection On Nutrient Allocation And Fecundity In Drosophila Melanogaster, Bryan Penalosa, Allen Gibbs
Effects Of Starvation Selection On Nutrient Allocation And Fecundity In Drosophila Melanogaster, Bryan Penalosa, Allen Gibbs
Undergraduate Research Opportunities Program (UROP)
The life cycle of holometabolous insects is distinctly divided into three life stages: the larval, pupal, and adult stages. During the larval stage, animals accumulate energy stores in the larval fat body to be later used in the pupal and adult stages. I determined how this accumulated energy is stored in adult Drosophila melanogaster that have been selected for resistance to starvation for >40 generations. I assayed carbohydrates, proteins, and lipids and found that 0 day old starvation selected flies have almost four times as much lipid content, twice as much carbohydrate, and a fairly equal amount of protein versus …
Undergraduate Research Opportunities Program 2010, University Of Nevada, Las Vegas
Undergraduate Research Opportunities Program 2010, University Of Nevada, Las Vegas
Undergraduate Research Opportunities Program (UROP)
The Undergraduate Research Opportunities Program (UROP) cultivates and supports research partnerships and invites undergraduates to work as the junior colleagues of faculty. The program offers the opportunity to work on cutting edge research—whether you join established research projects or pursue your own ideas. As participants, undergraduates engage in each phase of standard research activity: developing research plans, writing proposals, conducting research, analyzing data. and presenting research results in oral and written form. The projects take place over the summer, and research can be done in any academic department or interdisciplinary laboratory. Projects can last for an entire semester, and many …
Assessing Invasive Plant Species As Threats In Mojave Desert Parks, Sara L. Mcpherson, Donovan J. Craig, Scott R. Abella
Assessing Invasive Plant Species As Threats In Mojave Desert Parks, Sara L. Mcpherson, Donovan J. Craig, Scott R. Abella
Graduate Research Symposium (2010 - 2017)
The invasion and persistence of exotic plant species threatens the natural features that national parks are designed to protect. For example, park managers have witnessed an increase in the frequency, extent and intensity of fires along with a reduction in native species richness and diversity. Many park managers are familiar with a suite of highly invasive plants, but lack a comprehensive and systematic way of prioritizing invasive plant species based on potential threats to the parks’ resources. We have entered into a collaborative project with the National Park Service’s Inventory and Monitoring program to develop an invasive plant early detection …
29 Years Of Vegetation Community Change Across Environmental Gradients In A Mojave Desert Mountain Range, Christopher L. Roberts, James S. Holland, Scott R. Abella
29 Years Of Vegetation Community Change Across Environmental Gradients In A Mojave Desert Mountain Range, Christopher L. Roberts, James S. Holland, Scott R. Abella
Graduate Research Symposium (2010 - 2017)
There is a great deal of uncertainty as to how biological communities respond to changes in land use and climate change, a situation particularly relevant in protected areas such as national parks that were designated to conserve specific biological features. Utilizing extant vegetation data sets with repeatable methodology can provide opportunities for insight into previous vegetation change and provide base line data for long-term monitoring projects useful for modeling vegetation community trajectories. We have relocated and resurveyed 106 sites from a vegetation community study initiated in 1979 in the Newberry Mountains, southern Nevada, within Lake Mead National Recreation Area managed …
Waterfall Fire Interpretive Trail: Community College Fellowship, Mike Sady, Jay Arnone, Ann Bollinger, Alice Sady
Waterfall Fire Interpretive Trail: Community College Fellowship, Mike Sady, Jay Arnone, Ann Bollinger, Alice Sady
2010 Annual Nevada NSF EPSCoR Climate Change Conference
20 PowerPoint slides Convener: Franco Biondi, UNR & Michael Young, DRI Session 4: Ecological Change and Water Resources Abstract: Plant Surveys take place at each Study Site along the trail at intervals during the Spring and Summer. There are eight study sites that contain 3 plots each staked 2m x 2m square.
Water Source Partitioning For Shrubland Transpiration Using Innovative Field Methods, Dale A. Devitt, Michael Young, Matthew S. Lachniet, Jeremy Koonce, Amanda Wagner, Brian M. Bird, J. Healey
Water Source Partitioning For Shrubland Transpiration Using Innovative Field Methods, Dale A. Devitt, Michael Young, Matthew S. Lachniet, Jeremy Koonce, Amanda Wagner, Brian M. Bird, J. Healey
2010 Annual Nevada NSF EPSCoR Climate Change Conference
37 PowerPoint slides Convener: Franco Biondi, UNR & Michael Young, DRI Session 4: Ecological Change and Water Resources Abstract: -Climate change models predict a decline in precipitation over the next few decades throughout much of the southwest. -Such change has the potential to shift water uptake dynamics of phreatophytes -If groundwater pumping also occurs, the impact of climate change could be exacerbated. -A better understanding of the forces that drive the coupling and decoupling of phreatophytes to groundwater is needed.
Design And Status Of The Elevationl Transect And Monitoring Systems For Nevada’S Nsf Epscor Climate Change Research Program, Brian M. Bird, Scotty Strachan, David B. Simeral, Richard L. Jasoni
Design And Status Of The Elevationl Transect And Monitoring Systems For Nevada’S Nsf Epscor Climate Change Research Program, Brian M. Bird, Scotty Strachan, David B. Simeral, Richard L. Jasoni
2010 Annual Nevada NSF EPSCoR Climate Change Conference
15 PowerPoint slides Convener: Franco Biondi, UNR & Michael Young, DRI Session 4: Ecological Change and Water Resources Abstract: -Current status of Transects -Sheep Range Transect (5 sites) fully permitted through Fish and Wildlife Service. -Site access agreement acquired from the Long Now Foundation for Snake Range (sites 1, 2 and 3). -Snake Range site 4 access granted through the Nevada Land Conservatory. -Waiting for site permit approval on sites 8,9 and 6,7 from BLM and GBNP respectively. -Tower installed at North Las Vegas UWCC for testing of sensors and communications. -Installation of towers will begin winter 2010 at lower …
Biotic Responses To Climate Change In The Great Basin And Mojave Desert: Vegetation, David Charlet, Patrick Leary
Biotic Responses To Climate Change In The Great Basin And Mojave Desert: Vegetation, David Charlet, Patrick Leary
2010 Annual Nevada NSF EPSCoR Climate Change Conference
84 PowerPoint slides Convener: Franco Biondi, UNR & Michael Young, DRI Session 4: Ecological Change and Water Resources Abstract: -The Problem -To detect changes in vegetation as it responds to changes in climate, we must first know where the vegetation is now -GAP (Geographic Approach to Protection of Biodiversity) map --National project to map land cover throughout the US. --Nevada map (Edwards et al. 1996) -Southwest ReGAP map --Land cover map for southwestern US (Prior-Magee et al. 2007) to correct problems identified in GAP map
Geovisualization Of Ecological Data For Park Policy Support, Scott R. Abella, Haroon Stephen, Ross Guida
Geovisualization Of Ecological Data For Park Policy Support, Scott R. Abella, Haroon Stephen, Ross Guida
2010 Annual Nevada NSF EPSCoR Climate Change Conference
12 PowerPoint slides Convener: William Smith, UNLV Session 3: Policy, Decision Making, and Outreach Abstract: -Literature shows upward elevation shifts of biological species as a result of climate change -Effects of climate change expected to accelerate in coming decades -Concern both about species migrating out of parks and the potential inability of species to quickly adapt to the changing conditions within National Parks and other federal land boundaries
Research Poster: Physiological Responses Of Two Invasive Annual Grasses, Cheatgrass And Red Brome, In The Great Basin, L. Hernandez, R. Nowak, L. Salto
Research Poster: Physiological Responses Of Two Invasive Annual Grasses, Cheatgrass And Red Brome, In The Great Basin, L. Hernandez, R. Nowak, L. Salto
2010 Annual Nevada NSF EPSCoR Climate Change Conference
Research poster
Research Poster: Climate Change Impacts To Groundwater, Springs Hydrology And Aquatic Communities Amargosa Desert And Death Valley National Park, Nevada And California, Terry Fisk, Greg Pohll, Don Sada, Mark Stone
Research Poster: Climate Change Impacts To Groundwater, Springs Hydrology And Aquatic Communities Amargosa Desert And Death Valley National Park, Nevada And California, Terry Fisk, Greg Pohll, Don Sada, Mark Stone
2010 Annual Nevada NSF EPSCoR Climate Change Conference
Research poster
Research Poster: Biotic Responses To Climate Change In The Mojave Desert: Floristics, Patrick Leary, David Charlet
Research Poster: Biotic Responses To Climate Change In The Mojave Desert: Floristics, Patrick Leary, David Charlet
2010 Annual Nevada NSF EPSCoR Climate Change Conference
Research poster
Research Poster: Eco-Hydrological Pathways Inferred From Stable Isotopes In A Pinus Ponderosa And Pinus Monophylla Woodland Of The Sheep Range, Southern Great Basin, Usa, Kelli Hoover, Simon Poulson, Franco Biondi, Jeffrey Underwood
Research Poster: Eco-Hydrological Pathways Inferred From Stable Isotopes In A Pinus Ponderosa And Pinus Monophylla Woodland Of The Sheep Range, Southern Great Basin, Usa, Kelli Hoover, Simon Poulson, Franco Biondi, Jeffrey Underwood
2010 Annual Nevada NSF EPSCoR Climate Change Conference
Research poster
Research Poster: Clark County School District Involvement In The Nsf Epscor Program, Education Component, Aubrey M. Shirk, Larry Rudd, Paul Buck
Research Poster: Clark County School District Involvement In The Nsf Epscor Program, Education Component, Aubrey M. Shirk, Larry Rudd, Paul Buck
2010 Annual Nevada NSF EPSCoR Climate Change Conference
Research poster
Research Poster: Climate Change Science For Northern Nevada Educators, Melissa Slayden, Jacque Ewing-Taylor, Kelly Cannon
Research Poster: Climate Change Science For Northern Nevada Educators, Melissa Slayden, Jacque Ewing-Taylor, Kelly Cannon
2010 Annual Nevada NSF EPSCoR Climate Change Conference
Research poster