Life Sciences Commons^™

Telorchis Gutturosi Sp. N. (Trematoda: Telorchiidae) From Graptemys Pseudogeographica Gray In Nebraska, With Reports Of Additional Species Of Trematodes From Nebraska Turtles, Daniel R. Brooks, Monte A. Mayes

Harold W. Manter Laboratory of Parasitology: Faculty and Staff Publications

One species of digenean is described and 8 others reported from turtles in Nebraska. Telorchis gutturosi, described from Graptemys pseudogeographica, resembles T. stossichi, T. pseudoaculeatus, and T. pleroticus in having an acetabulum significantly smaller than the oral sucker, but differs by possessing a pharynx which is as large as the acetabulum and having the ovary only 1/10 the body length from the acetabulum. Telorchis necturi is considered the senior synonym of T. cryptobranchi; Graptemys pseudogeographica is a new host. Hapalorhynchus stunkardi is reported for the first time since its description. Based on new morphological evidence, …

Two Tetraphyllidean Cestodes From The Freshwater Stingray Potamotrygon Magdalenae Dumeril 1852 (Chondrichthyes: Potamotrygonidae) From Colombia, Daniel R. Brooks, Thomas B. Thorson

Harold W. Manter Laboratory of Parasitology: Faculty and Staff Publications

Two new tetraphyllidean cestodes are described from the freshwater stingray Potamotrygon magdalenae from Colombia. The new genus Potamotrygonocestus is proposed for P. magdalenensis, which differs from Pedibothrium by having a bilobed rather than X-shaped ovary, and simple rather than bifid hooks. It further differs from Pachybothrium hutsoni by having bothridial hooks inserted immediately posterior to an accessory sucker rather than inserted in the middle of a thick muscular pad. Potamotrygonocestus magdalenensis is unique among known tetraphyllideans by possessing vitellaria which are compact, cylindrical bands in the lateral portions of the middle of the proglottid. The second new species, Rhinebothrium …

Ultrastructure Of The Sporocyst Wall During Excystation Of Isospora Endocallimici, Clarence A. Speer, Alan A. Marchiondo, Donald W. Duszynski, Sharon K. File

Harold W. Manter Laboratory of Parasitology: Faculty and Staff Publications

Sporocysts of Isospora endocallimici, a parasite of marmosets, were exposed to minimal essentials medium (MEM) or a trypsin-bile salt solution (TBS) and then fixed and prepared for trans- mission electron microscopy. Excystation occurred in TBS but not MEM. The sporocyst wall has 2 layers, a thin outer layer (15 to 110 nm thick) and a thick inner layer (65 to 180 nm thick), which is composed of 4 separate curved plates. The outer layer consists of 1 to 3 membranes interspersed with lipid droplets. In the inner layer, a thin layer of material connects the peripheral margins of 2 …

Some Platyhelminth Parasites Of Sirens (Amphibia: Sirenidae) From North America, Daniel R. Brooks, Richard L. Buckner

Harold W. Manter Laboratory of Parasitology: Faculty and Staff Publications

A new genus and species of digenean and a new species of cestode are described and a previously described species of digenean reported for the first time from Siren intermedia from Illinois. A new genus is proposed for a previously described digenean, and another known species of digenean reported from Siren lacertina in Florida. Progorgodera foliata from S. intermedia has a foliate hind- body characteristic of members of Phyllodistomum and multiple testes arranged in 2 longitudinal rows characteristic of members of Gorgodera. Ophiotaenia sireni from the same host resembles O. filaroides in number of testes, O. amphiumae and O. …

Ultrastructure Of Interlamellar Henneguya Exilis In The Channel Catfish, William L. Current, John J. Janovy Jr.

John Janovy Publications

Ultrastructural aspects of interlamellar Henneguya exilis infections in channel catfish are reported. The plasmodium wall of this form differs from that of other species in that it is composed of two outer unit membranes which give rise to a zone of numerous pinocytic canals. Single-membraned canals appeared to be a stable feature of the wall while double-membraned canals are interpreted as those actively carrying out pinocytosis. Evidence suggests that host cellular cytoplasm as well as interstitial material is taken in by plasmodia. Plasmodium wall integrity, aggregation of parasite ectoplasmic components, numbers of pinocytic canals, and number of mitochondria proximal to …

Fabespora Vermicola Sp. N., The First Myxosporidan From A Platyhelminth, Robin M. Overstreet

Harold W. Manter Laboratory of Parasitology: Faculty and Staff Publications

The myxosporidan Fabespora vermicola sp. n., tentatively placed in the family Myxidiidae, is described from the digenean Crassicutis archosargi in an estuarine fish. The myxosporidan becomes the first known from a platyhelminth and the third from an invertebrate. It differs from the only other member of the genus primarily by having polar filaments 32 to 44 μm long rather than about 8 μm, and an infection with it can stop reproduction in the digenean host either by physical means or by necrosis of gametes.

A Redescription Of Crassicutis Archosargi, A Digenean Exhibiting An Unusual Tegumental Attachment, Robin M. Overstreet

Harold W. Manter Laboratory of Parasitology: Faculty and Staff Publications

The homalometrine Crassicutis archosargi from the northern Gulf of Mexico is redescribed, and some related digeneans are discussed. Numerous unidentified refractile bodies occurred in the tegument and other tissues of a few specimens of C. archosargi, and an intimate relationship between the parasite and its piscine host resulting from a modified tegument is reported for others. Binding by the described adhesive tegument increases the number of known ways digeneans attach to their hosts. Several specimens also harbored a histozoic myxosporidan in several tissues and a flagellate, Hexamita sp., in their ceca.

Anesthetization Of Morphologically Similar Species With Carbon Dioxide For Separation And Culture, Gregory R. Noel, Armand R. Maggenti

Harold W. Manter Laboratory of Parasitology: Faculty and Staff Publications

Nematologists frequently encounter congeneric species in sampling for nematodes. No convenient method for the separation of morphologically similar species has been described. For Meloidogyne spp., inoculum consisting of hundreds of eggs can be easily obtained from a single egg mass and a pure population reared on a suitable host. The separation and culture of other plant parasitic nematode species, such as Paratrichodorus spp., is often more difficult. The species may be separated by placing a single female in the rhizosphere of a host plant, but the chances of a single nematode increasing to a high population in a short time …

Neodeuterobaris Pritchardae Gen. Et Sp. N. (Digenea: Microscaphidiidae) In A Sideneck Turtle, Podocnemis Lewyana Dumeril 1852, From Colombia, Daniel R. Brooks

Harold W. Manter Laboratory of Parasitology: Faculty and Staff Publications

Neodeuterobaris pritchardae in the stomach of the freshwater turtle, Podocnemis lewyana, from Colombia resembles members of Deuterobaris in possessing ventral glands, a dorsal oral hood, and vitelline follicles confluent posteriorly, but apparently differs from them by possessing 5 rather than 7 rows of ventral glands; by lacking ventral spines, extracecal uterine loops, and a muscular esophageal bulb; and by inhabiting the stomach of freshwater turtles rather than the intestine of marine turtles. Those characters are considered significant enough to erect a new genus. Diagnosis of the Deuterobaridinae is emended to accommodate the new species.

Five Species Of Platyhelminths From Bufo Marinus L. (Anura: Bufonidae) In Colombia With Descriptions Of Creptotrema Lynchi Sp. N. (Digenea: Allocreadiidae) And Glypthelmins Robustus Sp. N. (Digenea: Macroderoididae), Daniel R. Brooks

Harold W. Manter Laboratory of Parasitology: Faculty and Staff Publications

Gorgoderina diaster, Cylindrotaenia americana, and Ophiotaenia bonariensis are reported, and Creptotrema lynchi and Glypthelmins robustus described from Bufo marinus from 3 localities in the Republic of Colombia. All are new locality records and B. marinus is a new host for G. diaster and C. americana. Creptotrema lynchi differs from C. creptotrema in possessing smaller eggs, relatively smaller oral papillae and suckers, and elongate rather than spherical testes. Glypthelmins robustus differs from all other species in the genus in possessing a pharynx which is significantly larger than the acetabulum and tegumental spines restricted to the anterior fifth of …

Parasites Of California Marine And Estuarine Fish, Milton S. Love, Mike Moser

Harold W. Manter Laboratory of Parasitology: Faculty and Staff Publications

While working on the parasites of several California marine fish, it was difficult to find out which parasites had been reported from our fish. Pertinent sources were scattered over a number of journals, covering many years. We compiled this list because we felt there was a need for a single source which would give all the parasites infecting California marine fish. As "marine fish" was a somewhat vague term, we decided to use it in a broad sense and included estuarine and anadromous fish in the survey. In addition, we have included records from landlocked populations of anadromous fish, such …

Prevalence Of Antibodies To Toxoplasma Gondii In Wild And Domestic Animals Of New Mexico, Arizona And Colorado, Alan A, Marchiondo, Donald W. Duszynski, Gary O. Maupin

Harold W. Manter Laboratory of Parasitology: Faculty and Staff Publications

Using the Sabin-Feldman dye test, sera from wild and domestic animals in New Mexico, Arizona and Colorado were tested for the prevalence of antibodies to Toxoplasma gondii. The prevalence of positive titers (≥ 1:8) in animals from these areas was: New Mexico (178 of 569, 31%), Arizona (11 of 56, 20%), and Colorado (2 of 7, 29%). The overall prevalence of antibodies to Toxoplasma was 30% (191 of 632).

Nine of 17 fecal samples from wild zoo felines contained Toxoplasma-like oocysts which were inoculated per os and intraperitoneally into mice. Mice from six of these nine inoculations later …

Immunoglobulins Attached To And In The Integument Of Adult Schistosoma Mansoni Sambon 1907, From First Infection Of Cf1 Mice, Franklin Sogandares-Bernal

Harold W. Manter Laboratory of Parasitology: Faculty and Staff Publications

7S_γ2b antibody was found attached to and in the integument of adult Schistosoma mansoni removed from CF₁ white mice. With the techniques used, I_gA, I_gM, 7S_γ1, 7S_γ2a, 7S_γ3, and C3 complement were not found to be attached to the integumental surfaces. 7S_γ2b does not seem to fix C3, and it is suggested this antibody may be acting in enhancing and blocking roles which protect the worms from the host.