Life Sciences Commons^™

A New Genus And Sixteen New Species Of False Click Beetles (Coleoptera: Eucnemidae) Described From The Heredia Province Of Costa Rica With Several Additional Records From The Osa Peninsula And Panama, Robert L. Otto, Jyrki Muona, Jim Córdoba-Alfaro

Insecta Mundi

More than 1300 specimens of Eucnemidae collected from Heredia Province in Costa Rica during the 1990s Arthropods of La Selva (ALAS) survey were studied from 2018 through 2022. One new genus of false click beetle, Absensiugum Otto, Muona and Córdoba-Alfaro, is described. Nematodes teres Horn, from the Ne­arctic and Caribbean regions, is transferred to this new genus to form Absensiugum teres, new combination. Sixteen new species of false click beetle (Coleoptera: Eucnemidae) are described from Costa Rica. These new species are: Adelothyreus brevis, Adelothyreus costaricensis, Adelothyreus totus, Quirsfeldia stethonoides, Lacus pectinatus, Maelodrus costaricensis, …

Helminth Parasites Of The Giant Toad, Rhinella Horribilis (Wiegmann, 1833) (Anura: Bufonidae) From Central Mexico, Jorge Falcón-Ordaz, Cristian Raúl Olvera-Olvera, Marisol Moreno-Chávez, Griselda Pulido-Flores, Scott Monks, Luis García-Prieto

MANTER: Journal of Parasite Biodiversity

As part of an ongoing project to inventory the helminth parasites of amphibians from the state of Hidalgo, Central Mexico, specimens of Rhinella horribilis were collected from three municipalities: Eloxochitlán, Huehuetla, and San Felipe Orizatlán. A total of eight taxa of helminths were found: three digeneans (Haematoloechus sp., Langeronia macrocirra, and Mesocoelium danforthi) and five nematodes (Cosmocerca sp., Cruzia morleyi, Ochoterenella chiapensis, Oswaldocruzia subauricularis, and Rhabdias sp.). The highest species richness was recorded in Huehuetla. All reports of these species of helminths represent new locality records for Mexico.

Demographic, Environmental And Physiological Predictors Of Gastrointestinal Parasites In Urban Raccoons, Liana F. Wait, Shylo R. Johnson, Kathleen M. Nelson, Richard B. Chipman, Frederick E. Pogmore, Andrew P. Dobson, Andrea L. Graham

USDA Wildlife Services: Staff Publications

Raccoons are host to diverse gastrointestinal parasites, but little is known about the ecology of these parasites in terms of their interactions with each other during coinfections, their interactions with host physiology and environmental factors, and their impact on raccoon health and survival. As a first step, we investigated the patterns of parasite infection and their demographic distribution in an urban-suburban population of raccoons trapped in the summers and autumns of 2018 and 2019. We collected faecal samples, demographic data, morphometric measurements, and blood smears, and used GPS data to classify trapping location by land cover type. Faecal floats were …

18s-Nemabase: Curated 18s Rrna Database Of Nematode Sequences, Kaitlin Gattoni, Eli M. S. Gendron, Rebeca Sandoval- Ruiz, Abigail Borgemeier, J. Parr Mcqueen, Rachel M. Shepherd, Dieter Slos, Tom Powers, Dorota L. Porazinska

Department of Plant Pathology: Faculty Publications

Nematodes are the most abundant and diverse animals on the planet but lack representation in biodiversity research. This presents a problem for studying nematode diversity, particularly when molecular tools (i.e., barcoding and metabarcoding) rely on well-populated and curated reference databases, which are absent for nematodes. To improve molecular identification and the assessment of nematode diversity, we created and curated an 18S rRNA database specific to nematodes (18S-NemaBase) using sequences sourced from the most recent publicly available 18S rRNA SILVA v138 database. As part of the curation process, taxonomic strings were standardized to contain a fixed number of taxonomic ranks relevant …

18s-Nemabase: Curated 18s Rrna Database Of Nematode Sequences, Kaitlin Gattoni, Eli M. S. Gendron, Rebeca Sandoval- Ruiz, Abigail Borgemeier, J. Parr Mcqueen, Rachel M. Shepherd, Dieter Slos, Tom Powers, Dorota L. Porazinska

Department of Plant Pathology: Faculty Publications

Nematodes are the most abundant and diverse animals on the planet but lack representation in biodiversity research. This presents a problem for studying nematode diversity, particularly when molecular tools (i.e., barcoding and metabarcoding) rely on well-populated and curated reference databases, which are absent for nematodes. To improve molecular identification and the assessment of nematode diversity, we created and curated an 18S rRNA database specific to nematodes (18S-NemaBase) using sequences sourced from the most recent publicly available 18S rRNA SILVA v138 database. As part of the curation process, taxonomic strings were standardized to contain a fixed number of taxonomic ranks relevant …

Novel Information On The Morphology, Phylogeny And Distribution Of Camallanid Mematodes From Marine And Freshwater Hosts In South Africa, Including The Description Of Camallanus Sodwanaensis N. Sp., Roman Svitin, Marliese Truter, Olena Kudlai, Nico J. Smit, Louis Du Preez

Harold W. Manter Laboratory: Library Materials

Four species of previously known nematodes from the family Camallanidae were found from different hosts in South Africa: Batrachocamallanus xenopodis from the frog Xenopus muelleri, Paracamallanus cyathopharynx, and Procamallanus pseudolaeviconchus from the catfish Clarias gariepinus and Spirocamallanus daleneae from the catfish Synodontis zambezensis. In the material collected from various marine fishes, several specimens of nematodes from the genus Camallanus clearly differed from all previously known species. Based on morphological differences these specimens are assigned to a new species, C. sodwanaensis. Molecular data of 18S and 28S rDNA and COI sequences are provided for the collected species …

Nathan Augustus Cobb: The Father Of Nematology In The United States; And Nathan Cobb's Publications, R. N. Huettel, A. Morgan Golden, Donna Ellington

Harold W. Manter Laboratory of Parasitology: Faculty and Staff Publications

Introduction

Nathan Augustus Cobb, referred to as the "Father of Nematology in the United States" (56), was a Renaissance man and a man of humble beginnings. His early life experiences instilled in him the creativity, fortitude, and self reliance that were needed to raise himself alone from age 14, to complete college with little formal pre-college education, receive his Ph.D. in Germany, and go on to found a new discipline of science called "Nematology". His scientific and technical accomplishments were impressive, as indicated by the over 250 first-authored manuscripts he produced during his career. He identified over 1000 species of …

Checklist Of Helminths Of Bats From Mexico And Central America, F. Agustín Jiménez, Juan M. Caspeta-Mandujano, Said B. Ramírez-Chávez, Silvia E. Ramírez-Díaz, Marissa G. Juárez-Urbina, Jorge L. Peralta-Rodríguez, José A. Guerrero

MANTER: Journal of Parasite Biodiversity

Based on original data obtained from fieldwork conducted from January 2008 to December 2015 and from previous records from published accounts, an updated checklist of helminth parasites of bats from Mexico and Central America is presented. The checklist has been organized in four ways, first as a helminth-host list in the state of Morelos, second as a helminth-host list with taxonomic and geographic distributional information, third as a bat-helminth list with references, and last, as a summary of the host-helminth association. A total of 105 records and 67 helminth taxa (26 trematodes, 4 cestodes, and 37 nematodes [33 adult and …

Descriptions Of Six New Species Of False Click Beetles (Coleoptera: Eucnemidae: Macraulacinae), With New Identification Keys For One Tribe And Two Genera, Robert L. Otto

Insecta Mundi

Six new species of false click beetle (Coleoptera: Eucnemidae) are described. These new species are: Fornax dixiensis sp. nov. (Florida, USA), Dromaeolus comayaguiensis sp. nov. (Honduras), Asiocnemis bicolor sp. nov. (Ecuador), Miruantennus chinensis sp. nov. (Yunnan prov., China), Miruantennus cuneiformis sp. nov. (Malaysia) and Nematodes africanus sp. nov. (Côte d’Ivoire). Identifi cation keys are provided for species of Fornax in the Nearctic ecozone and Miruantennus in the Palearctic and Indo-Malayan ecozones. A new key to include all the genera within the tribe Nematodini Leiler is also provided.

On The Helminth Fauna Of Small Mammals In Hustai National Park, Mongolia, Sumiya Ganzorig, Nyamsuren Batsaikhan, Katsuro Hagiwara, Scott Lyell Gardner, Kenji Baba, Buho Hoshino

Scott L. Gardner Publications

Introduction

The Hustai National Park is one of the special protected areas in Mongolia. The park is famous by the successful re-introduction of the wild horse (takhi), and its well-managed protection work. The park has a rich and diverse mammal fauna and flora. Of these, the takhi, and other large mammal species are objects of intensive research by the international researchers, but the parasitic helminth fauna in Hustai National Park is remained unknown. Species diversity of wildlife and its abundance, especially high density of both the prey and predators, might allow the presence of various parasitic and symbiotic organisms on …

Effect Of Prey Richness On A Consumer’S Intrinsic Growth Rate, Brian J. Darby, Michael A. Herman

School of Biological Sciences: Faculty Publications

The intrinsic growth rate of nonselective microbivores increases asymptotically with increasing prey biomass, but we do not know how intrinsic growth rate is affected by prey richness. The objective of this experiment was to determine the effect of prey richness on the growth kinetics of nematode predators while grazing on mixed bacterial lawns. We found that the intrinsic growth rate of Caenorhabditis elegans in laboratory culture increased asymptotically with prey richness. The mechanism of this pattern was primarily due to the best available prey species in the mixture: the intrinsic growth rate of the consumer feeding on a mixture of …

High-Throughput Amplicon Sequencing Of Rrna Genes Requires A Copy Number Correction To Accurately Reflect The Effects Of Management Practices On Soil Nematode Community Structure, B. J. Darby, T. C. Todd, M. A. Herman

School of Biological Sciences: Faculty Publications

Nematodes are abundant consumers in grassland soils, but more sensitive and specific methods of enumeration are needed to improve our understanding of how different nematode species affect, and are affected by, ecosystem processes. High-throughput amplicon sequencing is used to enumerate microbial and invertebrate communities at a high level of taxonomic resolution, but the method requires validation against traditional specimen-based morphological identifications. To investigate the consistency between these approaches, we enumerated nematodes from a 25-year field experiment using both morphological and molecular identification techniques in order to determine the long-term effects of annual burning and nitrogen enrichment on soil nematode communities. …

A New Species Of Dipetalonema (Filarioidea: Onchocercidae) From Ateles Chamek From The Beni Of Bolivia, Juliana Notarnicola, F. Agustin Jimenez-Ruiz, Scott Lyell Gardner

Harold W. Manter Laboratory of Parasitology: Faculty and Staff Publications

We describe a new species of Dipetalonema occurring in the body cavity of Ateles chamek (Humboldt, 1812) from north-central Bolivia. Morphologic characters serving to separate Dipetalonema yatesi n. sp. from known forms include a vagina vera with a simple tube and thin walls and a left spicule, which possesses a handle shorter than the lamina (ratio 2.7); the latter displays an anterior membranous alae similar in length to the terminal flagellum, a distal extremity of the left spicule within a simple hook and a membrane, phasmids at the basis of the lappets, and heterogeneous muscles occupying the whole cavity. Dipetalonema …

Tadaridanema Delicatus (Schwartz, 1927) N. Gen., N. Comb. (Trichostrongylina: Molineidae) Parasite Of Molossidae Bats, Jorge Falcon-Ordaz, Carmen Guzman-Cornejo, Luis Garcia-Prieto, Scott Lyell Gardner

Harold W. Manter Laboratory of Parasitology: Faculty and Staff Publications

On the basis of the revision of the type material of Anoplostrongylus delicatus Schwartz, 1927, and new specimens collected from Tadarida brasiliensis mexicana (Saussure, 1860) in 4 arid localities from Mexico, we describe a new genus (Tadaridanema n. gen.), to which A. delicatus is transferred (as Tadaridanema delicatus (Schwartz, 1927) n. gen., n. comb.). This new genus differs from all other genera included in noplostrongylinae by having ray 2 larger than ray 3. In addition, T. delicatus can be differentiated from the type species of Anoplostrongylus (Anoplostrongylus paradoxus (Travassos, 1918)) because it possess vestibular branches equal in length, cephalic inflation …

Aspidoderidae From North America, With The Description Of A New Species Of Aspidodera (Nematoda: Heterakoidea), F. Agustin Jimenez-Ruiz, Scott Lyell Gardner, Andrea S. Varela-Stokes

Harold W. Manter Laboratory of Parasitology: Faculty and Staff Publications

Aspidodera sogandaresi n. sp. (Heterakoidea: Aspidoderidae) from Dasypus novemcinctus Linnaeus, 1758 is herein described. This nematode occurs in armadillos from as far south as the canal zone of Panama, north through central Mexico, and into the southern United States. Previously identified as Aspidodera fasciata (Schneider, 1866), this new species has blunt projections on the lips and lateral expansions at the distal tips of the spicules, whereas A. fasciata has conspicuous digitiform projections on the lips, and a terminal round expansion at the tips of the spicules. Other species of the family present in North America include Aspidodera binansata Railliet and …

Research Note: First Record Of Ancylostoma Malayanum (Alessandrini, 1905) From Brown Bears (Ursus Arctos L.), Mitsuhiko Asakawa, Scott Lyell Gardner, Tsutomu Mano

Harold W. Manter Laboratory of Parasitology: Faculty and Staff Publications

This is the first report of Ancylostoma malayanum (Alessandrini, 1905), subgenus Ceylancylostoma (Lichtenfels, 1980) from wild brown bears Ursus arctos L. The bears were collected by shooting in Wassamu-Cho province (44 [degrees] 80 [minutes] 59 [seconds] N, 142 [degrees] 82 [minutes] 99 [seconds] E), Hokkaido, Japan, November, 2003.

Effect Of Foliar Applied Plant Elicitors On Microbial And Nematode Populations In The Root Zone Of Potato, H. P. Collins, D. A. Navare, E. Riga, F. J. Pierce

United States Department of Agriculture-Agricultural Research Service / University of Nebraska-Lincoln: Faculty Publications

Systemic acquired resistance (SAR) is a process whereby a plant that successfully
resists a pathogen becomes highly resistant to subsequent infection not only by the original pathogen but also by a wide variety of pathogens. Most SAR research has focused on resistance in leaves, so much less is known about the effectiveness of foliar applications of SAR compounds in the protection of plant roots and associated microorganisms in soil. This study was conducted to determine if foliar SAR-inducing applications (BTH or harpin) negatively impact the potato root system beneficial rhizosphere microbial populations and activity or influence pathogenic nematode populations. Foliar …

A New Species Of Pelecitus (Filarioidea: Onchocercidae) From The Endnagered Tehuantepec Jackrabbit Lepus Flavigularis, F. Agustin Jimenez-Ruiz, Scott Lyell Gardner, Fernando A. Cervantes, Consuelo Lorenzo

Harold W. Manter Laboratory of Parasitology: Faculty and Staff Publications

Pelecitus meridionaleporinus n. sp. from the Tehuantepec jackrabbit is described. The new species differs from Pelecitus helicinus (Molin, 1860) in having delicate transverse striations, a salient vulva, and a readily apparent preesophageal ring; P. helicinus has teardrop cells around the vulva, which are lacking in the species presently described. The new species is different from Pelecitus scapiceps (Leidy, 1886) in having the vulva anterior to the esophageal–intestinal junction and wider lateral alae. Pelecitus scapiceps is found in the tarsal bursa of the hind feet of lagomorphs, whereas P. helicinus is found around tendons of legs and feet of birds. Pelecitus …

The Nematode Fauna Of Long-Nosed Mice Oxymycterus Spp. From The Bolivian Yungas, F. Agustin Jimenez-Ruiz, Scott Lyell Gardner

Harold W. Manter Laboratory of Parasitology: Faculty and Staff Publications

During a long-term survey of the parasites of mammals from all over Bolivia, 44 individuals of Oxymycterus inca and 6 of Oxymycterus paramensis were collected and examined for parasites from the foothills and Eastern Cordillera (Los Yungas) of the Andes of Bolivia. Three species of nematode were found including, from the cecum, a previously unknown genus and species of pinworm, Caroloxyuris boliviensis n. gen, n. sp., aspidoderids representing Nematomystes rodentophilus, and from the stomach, Protospirura numidica criceticola. These helminths occurred in prevalences of 25, 9, and 14%, respectively, in O. paramensis. Caroloxyuris boliviensis resembles species included in …

Ec01-1880 Diseases Of Nebraska's Field Crops, Loren J. Giesler, Robert M. Harveson, Jim Stack, John E. Watkins, Jennifer L. Chaky

University of Nebraska-Lincoln Extension: Historical Materials

Each year, plant diseases are an important factor in reducing yields of Nebraska's field crops. The purpose of this publication is to provide concise information in an easy to use table for many of Nebraska's field crop diseases. The disease information is organized by crop.

Diseases covered in this publication include the four main groups of plant pathogens: fungi, bacteria, viruses, and nematodes.

Worms, Nematoda, Scott Lyell Gardner

Harold W. Manter Laboratory of Parasitology: Faculty and Staff Publications

Nematodes are the most speciose phylum of metazoa on earth. Not only do they occur in huge numbers as parasites of all known animal groups, but also they are found in the soils, as parasites of plants, and in large numbers in the most extreme environments, from the Antarctic dry valleys to the benthos of the ocean. They are extremely variable in their morphological characteristics, with each group showing morphological adapta­tions to the environment that they inhabit. Soil-dwelling forms are extremely small; many marine species have long and complex setae; and parasitic species man­ifest amazingly great reproductive potential and large …

Arthropod And Helminth Parasites From The Plains Pocket Gopher, Geomys Bursarius Bursarius From The Hosts' Northern Boundary Range In Minnesota, Monroe H. Bartel, Scott Lyell Gardner

Harold W. Manter Laboratory of Parasitology: Faculty and Staff Publications

As part of a continuing and more general study of the diversity of parasites from subterranean mammals in central North America, individuals of the Plains pocket gopher, Geomys bursarius bursarius, were collected from 7 localities in northwestern Minnesota from September 1991 through October 1996. Arthropods collected included the fleas, Opisocrostis bruneri (4 of 124, 3.20/0), Foxella ignota ignota (85 of 124, 68.50/0); the chewing louse, Geomydoecus geomydis geomydis from 98 of 124 (790/0), and larvae of the tick, Dermacentor variabilis (1 of 124, 0.8%). Nematodes found included Physaloptera limbata (2 of 118 gophers, 1.7%), Capillaria americana (4 of 118, …

Molecular Phylogeny Of Clade Iii Nematodes Reveals Multiple Origins Of Tissue Parasitism, Steven A. Nadler, R. A. Carreno, H. Mejía-Madrid, J. Ullberg, C. Pagan, R. Houston, Jean-Pierre Hugot

Harold W. Manter Laboratory of Parasitology: Faculty and Staff Publications

Molecular phylogenetic analyses of 113 taxa representing Ascaridida, Rhigonematida, Spirurida and Oxyurida were used to infer a more comprehensive phylogenetic hypothesis for representatives of ‘clade III’. The posterior probability of multiple alignment sites was used to exclude or weight characters, yielding datasets that were analysed using maximum parsimony, likelihood, and Bayesian inference methods. Phylogenetic results were robust to differences among inference methods for most high-level taxonomic groups, but some clades were sensitive to treatments of characters reflecting differences in alignment ambiguity. Taxa representing Camallanoidea, Oxyurida, Physalopteroidea, Raphidascarididae, and Skrjabillanidae were monophyletic in all 9 analyses whereas Ascaridida, Ascarididae, Anisakidae, Cosmocercoidea, …

G96-1289 Diseases Of Home Garden Strawberries, John E. Watkins, David S. Wysong

University of Nebraska-Lincoln Extension: Historical Materials

This NebGuide addresses on some of the common diseases affecting home garden strawberries in Nebraska and their control.

Strawberries are a favored fruit of home gardens. They have been adapted to a wide range of environmental conditions, but sometimes can be challenging to grow. Strawberry yields and fruit quality are influenced by interactions of environment, diseases, pests, and soil conditions.

Body Size Evolution Of Oxyurid (Nematoda) Parasites: The Role Of Hosts, Serge Morand, Pierre Legendre, Scott Lyell Gardner, Jean-Pierre Hugot

Harold W. Manter Laboratory of Parasitology: Faculty and Staff Publications

Studying the diversification of body size in a taxon of parasites allows comparison of patterns of variation observed in the parasites with patterns found in free-living organisms. The distributions of body size of oxyurid nematodes (obligate parasites of vertebrates and invertebrates) are lognormally right-skewed, except for male oxyurids in invertebrates which show left-skewed distributions. In these parasitic forms, speciose genera do not have the smallest body sizes. Parasite body size is positively correlated with host body size, the largest hosts possessing the largest parasites. This trend is shown to occur within one monophyletic group of oxyurids, those of Old World …

A New Species Of Vexillata (Nemata: Ornithostrongylidae) From The Coarse-Haired Pocket Mouse Chaetodipus Hispidus In New Mexico, Scott Lyell Gardner, Edward B. Fong, Luma Al-Banna, Stacie R. Raymond

Harold W. Manter Laboratory of Parasitology: Faculty and Staff Publications

Individuals of a new species of trichostrongyloid nematode of the genus Vexillata were collected from the intestines of the coarse-haired pocket mouse, Chaetodipus hispidus, from eastern New Mexico. This new species possesses general features of the genus Vexillata but may be recognized as distinct from all other species by unique features of the dorsal ray of the caudal bursa and the synlophe.

Development Of Multiple Pest Resistant Crop Cultivars, Elvis A. Heinrichs

Department of Entomology: Faculty Publications

Insects are one, among a number, of biotic and abiotic constraints that limit the production of food crops. Entomologists can play a key role in increasing food production through the development of insect-resistant crop cultivars. Resistant cultivars are sought as a major tactic in the development of IPM strategies and have been shown to be compatible with biological, chemical and cultural control tactics. There has been significant progress in the breeding and commercial utilization of multiple pest resistant crop cultivars having resistance to insects, diseases and nematodes. The most notable examples are rice cultivars which are grown on millions of …

An Alternative Approach For Evaluating The Efficacy Of Potential Biocontrol Agents Of Weeds. 2. Path Analysis, Dan J. Pantone, William A. Williams, Armand R. Maggenti

Harold W. Manter Laboratory of Parasitology: Faculty and Staff Publications

Path analysis was used to assess the efficacy of the fiddleneck flower gall nematode as a weed biocontrol agent of coast fiddleneck in competition with wheat during two years of field experiments. The path analysis revealed that the number of inflorescences/plant for fiddleneck and the number of heads/plant for wheat were the most important yield components that determine fecundity and seed yield. The density of fiddleneck had a much greater impact on the yield components of fiddleneck than did the density of wheat or the nematode rate of inoculation. The nematode had its greatest negative impact on the number of …

An Alternative Approach For Evaluating The Efficacy Of Potential Biocontrol Agents Of Weeds. 2. Path Analysis, Dan J. Pantone, William A. Williams, Armand R. Maggenti

Harold W. Manter Laboratory of Parasitology: Faculty and Staff Publications

Path analysis was used to assess the efficacy of the fiddleneck flower gall nematode as a weed biocontrol agent of coast fiddleneck in competition with wheat during 2 yr of field experiments. The path analysis revealed that the number of inflorescences/plant for fiddleneck and the number of heads/plant for wheat were the most important yield components that determine fecundity and seed yield. The density of fiddleneck had a much greater impact on the yield components of fiddleneck than did the density of wheat or the nematode rate of inoculation. The nematode had its greatest negative impact on the number of …

An Alternative Approach For Evaluating The Efficacy Of Potential Biocontrol Agents Of Weeds. 1. Inverse Linear Mode, Dan J. Pantone, William A. Williams, Armand R. Maggenti

Harold W. Manter Laboratory of Parasitology: Faculty and Staff Publications

Methods for evaluating the efflicacy of potential classical biocontrol agents were outlined for a model biocontrol agent-weed-crop system. A proposed biocontrol agent (the fiddleneck flower gall nematode), its weed host (coast fiddleneck), and wheat were used as representative organisms. An additive experimental design (inverse linear model) was used. Regression of the reciprocal of the average plant biomass of each species onto the density of itself and the other plant species yielded competitive indices that measure the competitive ability of the plants. The results of 2 yr of field experiments revealed a dramatic change in the competitive interaction between fiddleneck and …