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Decapitation Impacting Effect Of Topically Applied Chlorpyrifos On Acetylcholinesterase And General Esterases In Susceptible And Resistant German Cockroaches (Dictyoptera: Blattellidae), No-Joong Park, Shripat T. Kamble
Decapitation Impacting Effect Of Topically Applied Chlorpyrifos On Acetylcholinesterase And General Esterases In Susceptible And Resistant German Cockroaches (Dictyoptera: Blattellidae), No-Joong Park, Shripat T. Kamble
Department of Entomology: Faculty Publications
The effect of topically applied chlorpyrifos on acetylcholinesterase and other esterases in heads and decapitated bodies of CSMA and Crawford German cockroaches was examined with spectrophotometric enzyme assay and native polyacrylamide gel electrophoresis. The toxicity of chlorpyrifos was greatly reduced in decapitated CSMA male cockroaches with LD50 value 17.1-fold higher than that of normal CSMA cockroaches. Acetylcholinesterase activity from heads was significantly higher in the Crawford compared with the CSMA strain and did not change until 24 h after chlorpyrifos in vivo treatment in both strains. The p-nitrophenyl butyrate (NPB) esterase activities from both heads and decapitated bodies of …