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Enzymatic And Nonenzymatic Mechanisms For Ferric Leghemoglobin Reduction In Legume Root Nodules, Manuel Becana, Robert V. Klucas
Enzymatic And Nonenzymatic Mechanisms For Ferric Leghemoglobin Reduction In Legume Root Nodules, Manuel Becana, Robert V. Klucas
Department of Biochemistry: Faculty Publications
Evidence is presented for the operation in nodules of at least four systems for restoring functional ferrous leghemoglobin (Lb2+) from its inactive, ferric form. (i) Reduction of ferric leghemoglobin (Lb3+) by a reductase. The enzyme is a flavoprotein of 100 kDa with two equally sized subunits and exhibits a Km of 9 µM for soybean Lb3+ component a and a K. of 51 µM for NADH. NADPH is only 30% (initial velocities) as effective as NADH. Lb3+ reductase converts 215 nmol of Lb3+to Lb2+ •CO (or Lb2+ •O2 …
Primary Sequence Of The Escherichia Coli Fadba Operon, Encoding The Fatty Acid-Oxidizing Multienzyme Complex, Indicates A High Degree Of Homology To Eucaryotic Enzymes, Concetta C. Dirusso
Primary Sequence Of The Escherichia Coli Fadba Operon, Encoding The Fatty Acid-Oxidizing Multienzyme Complex, Indicates A High Degree Of Homology To Eucaryotic Enzymes, Concetta C. Dirusso
Department of Biochemistry: Faculty Publications
In Escherichia coli at least five enzyme activities required for the beta-oxidation of fatty acids are associated with a multienzyme complex composed of two subunits in α2β2 conformation (A. Pramanik et al., J. Bacteriol. 137:469-473, 1979). In the present work, the DNA sequence of the genes encoding these two subunits, fadB and fadA, has been determined. The direction of transcription was from fadB to fadA rather than from fadA to fadB, as suggested previously (S. K. Spratt et al., J. Bacteriol. 158:535-542, 1984). Only 10 nucleotides separated the coding sequences for the two peptides, confirming …