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Nucleotide Sequence Of The Fadr Gene, A Multifunctional Regulator Of Fatty Acid Metabolism In Escherichia Coli, Concetta C. Dirusso
Nucleotide Sequence Of The Fadr Gene, A Multifunctional Regulator Of Fatty Acid Metabolism In Escherichia Coli, Concetta C. Dirusso
Department of Biochemistry: Faculty Publications
The Escherichia coli fadR gene is a multifunctional regulator of fatty acid
and acetate metabolism. In the present work the nucleotide sequence of the
1.3 kb DNA fragment which encodes FadR has been determined. The coding
sequence of the fadR gene is 714 nucleotides long and is preceded by a typical
E. coli ribosome binding site and is followed by a sequence predicted to be
sufficient for factor-independent chain termination. Primer extension experiments
demonstrated that the transcription of the fadR gene initiates with an
adenine nucleotide 33 nucleotides upstream from the predicted start of
translation. The derived fadR peptide has …
Reduced Co2/O2 Specificity Of Ribulose-Bisphosphate Carboxylase/Oxygenase In A Temperature-Sensitive Chloroplast Mutant Of Chlamydomonas, Zhixiang Chen, Chris J. Chastain, Souhail R. Al-Abed, Raymond Chollet, Robert J. Spreitzer
Reduced Co2/O2 Specificity Of Ribulose-Bisphosphate Carboxylase/Oxygenase In A Temperature-Sensitive Chloroplast Mutant Of Chlamydomonas, Zhixiang Chen, Chris J. Chastain, Souhail R. Al-Abed, Raymond Chollet, Robert J. Spreitzer
Department of Biochemistry: Faculty Publications
The Chlamydomonas reinhardtii chloroplast mutant 68-4PP is phenotypically indistinguishable from wild type at 25°C but fails to grow photosynthetically at 35°C. It had about 30% of the wild-type level of ribulose-1,5-bisphosphate carboxylase/oxygenase (EC 4.1.1.39) holoenzyme and carboxylase activity when grown at 25°C, but less than 15% when grown at 35°C. Pulse-labeling with 35S showed that the decrease in enzyme level at the restrictive temperature was not a result of reduced synthesis of enzyme subunits. The CO2/O2 specificity factor (VcKo/VoKc, where Vc and Vo are Vmax …