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Mitochondrial Dna Rearrangement Associated With Fertility Restoration And Cytoplasmic Reversion To Fertility In Cytoplasmic Male Sterile Phaseolus Vulgaris L., Sally Ann Mackenzie, D. R. Pring, M. J. Bassett, C. D. Chase

Center for Plant Science Innovation: Faculty and Staff Publications

Restoration of pollen fertility to cytoplasmic male sterile (CMS) Phaseolus vulgaris by a nuclear restorer gene provides a system for studying nuclear-cytoplasmic interactions. Introduction of a nuclear restorer gene to this CMS line of P. vulgaris (CMS-Sprite) results in a mitochondrial genome rearrangement similar to that observed upon spontaneous cytoplasmic reversion to fertility. Three spontaneous heritable cytoplasmic revertants were derived from CMS-Sprite. Five fully fertile restored lines were also produced by using restorer line R-351 (BC₃F₃ populations). Comparison of the mitochondrial DNA restriction patterns of CMS-Sprite, the three fertile revertants, and the five restored lines revealed loss …