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Full-Text Articles in Life Sciences
Characterization Of Bartonella-Bacilliformis Flagella And Effect Of Antiflagellin Antibodies On Invasion Of Human Erythrocytes, David C. Scherer, Isaure Deburon-Connors, Michael F. Minnick
Characterization Of Bartonella-Bacilliformis Flagella And Effect Of Antiflagellin Antibodies On Invasion Of Human Erythrocytes, David C. Scherer, Isaure Deburon-Connors, Michael F. Minnick
Biological Sciences Faculty Publications
Bartonella bacilliformis is the etiologic agent of Oroya fever in humans. Flagellum-mediated motility has been postulated as a major virulence factor for invasion of host cells. To address this hypothesis, we purified and characterized flagella from strain KC584 and then assessed their role in human erythrocyte association and invasion. Electron microscopy of the flagellar preparation showed a high concentration of filaments with a mean wavelength of 800 nm. Sodium dodecyl sulfate-polyacrylamide gel electrophoresis, immunoblot analysis, and KBr density gradient centrifugation indicated that the flagellar filament is composed of a polypeptide of 42 kDa. The flagellin is partially (ca. 50%) resistant …
Affinity Panning Of A Library Of Peptides Displayed On Bacteriophages Reveals The Binding Specificity Of Bip, Sylvie Blond-Elguindi, Steven E. Cwirla, William J. Dower, Robert J. Lipshutz, Stephen R. Sprang, Joseph F. Sambrook, Mary Jane H. Gething
Affinity Panning Of A Library Of Peptides Displayed On Bacteriophages Reveals The Binding Specificity Of Bip, Sylvie Blond-Elguindi, Steven E. Cwirla, William J. Dower, Robert J. Lipshutz, Stephen R. Sprang, Joseph F. Sambrook, Mary Jane H. Gething
Biological Sciences Faculty Publications
We have used affinity panning of libraries of bacteriophages that display random octapeptide or dodecapeptide sequences at the N-terminus of the adsorption protein (plll) to characterize peptides that bind to the endoplasmic reticulum chaperone BiP and to develop a scoring system that predicts potential BiP-binding sequences in naturally occurring polypeptides. BiP preferentially binds peptides containing a subset of aromatic and hydrophobic amino acids in alternating positions, suggesting that peptides bind in an extended conformation, with the side chains of alternating residues pointing into a cleft on the BiP molecule. Synthetic peptides with sequences corresponding to those displayed by BiP-binding bacteriophages …
Identification Of A Protein In Several Borrelia Species Which Is Related To Ospc Of The Lyme Disease Spirochetes., Richard T. Marconi, D. Scott Samuels, Tom G. Schwan, Claude F. Garon
Identification Of A Protein In Several Borrelia Species Which Is Related To Ospc Of The Lyme Disease Spirochetes., Richard T. Marconi, D. Scott Samuels, Tom G. Schwan, Claude F. Garon
Biological Sciences Faculty Publications
Using oligonucleotide probes which have previously been shown to be specific for the ospC gene found in the Lyme disease spirochete species Borrelia burgdorferi, B. garinii, and group VS461, we detected an ospC homolog in other Borrelia species including B. coriaceae, B. hermsii, B. anserina, B. turicatae, and B. parkeri. In contrast to the Lyme disease spirochetes, which carry the ospC gene on a 26-kb circular plasmid, we mapped the gene in other Borrelia species to linear plasmids which varied in size among the isolates tested. Some isolates carry multiple copies of the gene residing on linear plasmids of different …
On A (Β-) Roll, S. R. Sprang
On A (Β-) Roll, S. R. Sprang
Biological Sciences Faculty Publications
No abstract provided.
Nucleotide Sequence And Comparison Of The 5s Ribosomal Rna Genes Of Rochalimaea Henselae, R-Quintana And Brucella Abortus, Michael F. Minnick, Gary L. Stiegler
Nucleotide Sequence And Comparison Of The 5s Ribosomal Rna Genes Of Rochalimaea Henselae, R-Quintana And Brucella Abortus, Michael F. Minnick, Gary L. Stiegler
Biological Sciences Faculty Publications
No abstract provided.
Breeding Ground Fidelity And Mate Retention In The Pacific Golden-Plover, Oscar W. Johnson, Peter G. Connors, Phillip L. Bruner, John L. Maron
Breeding Ground Fidelity And Mate Retention In The Pacific Golden-Plover, Oscar W. Johnson, Peter G. Connors, Phillip L. Bruner, John L. Maron
Biological Sciences Faculty Publications
We found male-biased site fidelity in Pacific Golden-Plovers (Phviah fulva)
on breeding grounds in western Alaska. Males (8 of 8) returned to the same territories annually, while few females (1 of 4) were seen in subsequent seasons. Nest sites in successive years were usually within 100 m, and the same nest cup may be used in more than one year. First-year birds of both sexes mated with older birds and also with each other, but first-year females may breed less commonly than first-year males. The numbers of nesting birds on our study sites varied sharply both within and …
Nucleotide Sequence Of The 5s Ribosomal Rna Gene Of Bartonella Bacilliformis, Michael F. Minnick
Nucleotide Sequence Of The 5s Ribosomal Rna Gene Of Bartonella Bacilliformis, Michael F. Minnick
Biological Sciences Faculty Publications
No abstract provided.
Transcriptional Analyses And Mapping Of The Ospc Gene In Lyme Disease Spirochetes, Richard T. Marconi, D. Scott Samuels, Claude F. Garon
Transcriptional Analyses And Mapping Of The Ospc Gene In Lyme Disease Spirochetes, Richard T. Marconi, D. Scott Samuels, Claude F. Garon
Biological Sciences Faculty Publications
In Lyme disease spirochetes, the ospC gene encodes a 22.7-kDa protein referred to as either the pC or the OspC protein. Using a variety of electrophoretic approaches followed by Southern blotting and probing with oligonucleotide probes, we mapped the ospC gene to a circular 26-kb plasmid. The ospC gene represents the first gene to be mapped to a circular plasmid in Lyme disease spirochetes. The occurrence of this gene in isolates belonging to each of the three Lyme disease-associated species, Borrelia burgdorferi, Borrelia garinii, and the VS461 group, was evaluated. The ospC gene was found to occur in all 21 …
Coumermycin A1 Inhibits Growth And Induces Relaxation Of Supercoiled Plasmids In Borrelia Burgdorferi, The Lyme Disease Agent, D. Scott Samuels, Claude F. Garon
Coumermycin A1 Inhibits Growth And Induces Relaxation Of Supercoiled Plasmids In Borrelia Burgdorferi, The Lyme Disease Agent, D. Scott Samuels, Claude F. Garon
Biological Sciences Faculty Publications
Coumermycin A1 is an inhibitor of DNA gyrase, an enzyme that catalyzes supercoiling of DNA and is required for bacterial DNA replication. We have investigated the activity of this coumarin antibiotic on Borrelia burgdorferi, a spirochete and the causative agent of Lyme disease. B. burgdorferi was more susceptible than many other eubacteria to coumermycin as well as novobiocin, another coumarin antibiotic; this contrasted with its relative resistance to the DNA gyrase inhibitors nalidixic acid, oxolinic acid, and ciprofloxacin. Coumermycin at 0.2 micrograms/ml inhibited the growth of B. burgdorferi B31 in BSK II medium. A 100-fold-lower concentration induced the relaxation of …
Speciation In Golden-Plovers, Pluvialis Dominica And P. Fulva: Evidence From The Breeding Grounds, Peter G. Connors, Brian J. Mccaffery, John L. Maron
Speciation In Golden-Plovers, Pluvialis Dominica And P. Fulva: Evidence From The Breeding Grounds, Peter G. Connors, Brian J. Mccaffery, John L. Maron
Biological Sciences Faculty Publications
Two forms of golden-plover have long been considered subspecies, Pluvialis
dominica dominica and P. d. fulva. Prior studies have shown differences between forms in breeding distributions, wintering distributions, plumage, morphology, molt, and maturation schedules. We report clear and consistent differences in breeding vocalizations and nesting habitat, and strict assortative mating in areas of sympatry in western Alaska. These results indicate a greater degree of differentiation between the forms than was previously appreciated. They are appropriately treated as separate species and should be referred to under the names Pluvialis dominica, for the American Golden-Plover, and Pluvialis fulva, for …
Effects Of Silvicultural Treatments On Forest Birds In The Rocky Mountains: Implications And Management Recommendations, Richard L. Hutto, Sallie J. Hejl, Charles R. Preston, Deborah M. Finch
Effects Of Silvicultural Treatments On Forest Birds In The Rocky Mountains: Implications And Management Recommendations, Richard L. Hutto, Sallie J. Hejl, Charles R. Preston, Deborah M. Finch
Biological Sciences Faculty Publications
The short-term effects of timber harvesting practices on landbird species vary widely among species. Thus, the maintenance of populations of all species will require a long-term management strategy that involves maintenance of a variety of habitats over a broad landscape.
Cytokine Structural Taxonomy And Mechanisms Of Receptor Engagement, Stephen R. Sprang, J. Fernando Bazan
Cytokine Structural Taxonomy And Mechanisms Of Receptor Engagement, Stephen R. Sprang, J. Fernando Bazan
Biological Sciences Faculty Publications
Seven discrete families of cytokines have been identified by sequence and structural analysis. Within this diverse set of protein folds, the hematopoietic growth factors and tumor necrosis factor-like cytokines display a remarkable degree of variation upon their respective topological frameworks. In addition, prototype X-ray structures of ligand-receptor complexes reveal two different strategies of receptor engagement.