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Full-Text Articles in Life Sciences
Cloning, Heterologous Expression, And Characterization Of A Βκ-Carrageenase From Marine Bacterium Wenyingzhuangia Funcanilytica: A Specific Enzyme For The Hybrid Carrageenan–Furcellaran, Siqi Cao, Yuying Zhang, Guangning Chen, Jingjing Shen, Jin Han, Yaoguang Chang, Hang Xiao, Changhu Xue
Cloning, Heterologous Expression, And Characterization Of A Βκ-Carrageenase From Marine Bacterium Wenyingzhuangia Funcanilytica: A Specific Enzyme For The Hybrid Carrageenan–Furcellaran, Siqi Cao, Yuying Zhang, Guangning Chen, Jingjing Shen, Jin Han, Yaoguang Chang, Hang Xiao, Changhu Xue
Food Science Department Faculty Publication Series
Carrageenan is a group of important food polysaccharides with high structural heterogeneity. Furcellaran is a typical hybrid carrageenan, which contains the structure consisted of alternative beta-carrageenan and kappa-carrageenan motifs. Although several furcellaran-hydrolyzing enzymes have been characterized, their specificity for the glycosidic linkage was still unclear. In this study, we cloned, expressed, and characterized a novel GH16_13 furcellaran-hydrolyzing enzyme Cgbk16A_Wf from the marine bacterium Wenyingzhuangia fucanilytica CZ1127. Cgbk16A_Wf exhibited its maximum activity at 50 degrees C and pH 6.0 and showed high thermal stability. The oligosaccharides in enzymatic products were identified by liquid chromatography coupled with high-resolution mass spectrometry (LC-HRMS) and …
Fungal Community Response To Long-Term Soil Warming With Potential Implications For Soil Carbon Dynamics, Gregory J. Pec, Linda T. A. Van Diepen, Melissa Knorr, A. Staurt Grandy, Jerry M. Melillo, Kristen M. Deangelis, Jeffery L. Blanchard, Serita D. Frey
Fungal Community Response To Long-Term Soil Warming With Potential Implications For Soil Carbon Dynamics, Gregory J. Pec, Linda T. A. Van Diepen, Melissa Knorr, A. Staurt Grandy, Jerry M. Melillo, Kristen M. Deangelis, Jeffery L. Blanchard, Serita D. Frey
Microbiology Department Faculty Publication Series
The direction and magnitude of climate warming effects on ecosystem processes such as carbon cycling remain uncertain. Soil fungi are central to these processes due to their roles as decomposers of soil organic matter, as mycorrhizal symbionts, and as determinants of plant diversity. Yet despite their importance to ecosystem functioning, we lack a clear understanding of the long-term response of soil fungal communities to warming. Toward this goal, we characterized soil fungal communities in two replicated soil warming experiments at the Harvard Forest (Petersham, Massachusetts, USA) which had experienced 5 degrees C above ambient soil temperatures for 5 and 20 …
Spatial Distribution Of Arctic Bacterioplankton Abundance Is Linked To Distinct Water Masses And Summertime Phytoplankton Bloom Dynamics (Fram Strait, 79°N), Magda G. Cardozo-Mino, Eduard Fadeev, Verena Salman-Carvalho, Antje Boetius
Spatial Distribution Of Arctic Bacterioplankton Abundance Is Linked To Distinct Water Masses And Summertime Phytoplankton Bloom Dynamics (Fram Strait, 79°N), Magda G. Cardozo-Mino, Eduard Fadeev, Verena Salman-Carvalho, Antje Boetius
Microbiology Department Faculty Publication Series
The Arctic is impacted by climate warming faster than any other oceanic region on Earth. Assessing the baseline of microbial communities in this rapidly changing ecosystem is vital for understanding the implications of ocean warming and sea ice retreat on ecosystem functioning. Using CARD-FISH and semi-automated counting, we quantified 14 ecologically relevant taxonomic groups of bacterioplankton (Bacteria and Archaea) from surface (0-30 m) down to deep waters (2,500 m) in summer ice-covered and ice-free regions of the Fram Strait, the main gateway for Atlantic inflow into the Arctic Ocean. Cell abundances of the bacterioplankton communities in surface waters varied from …
How Do Shipworms Eat Wood? Screening Shipworm Gill Symbiont Genomes For Lignin-Modifying Enzymes, Stefanos Stravoravdis, J. Reuben Shipway, Barry Goodell
How Do Shipworms Eat Wood? Screening Shipworm Gill Symbiont Genomes For Lignin-Modifying Enzymes, Stefanos Stravoravdis, J. Reuben Shipway, Barry Goodell
Microbiology Department Faculty Publication Series
Shipworms are ecologically and economically important mollusks that feed on woody plant material (lignocellulosic biomass) in marine environments. Digestion occurs in a specialized cecum, reported to be virtually sterile and lacking resident gut microbiota. Wood-degrading CAZymes are produced both endogenously and by gill endosymbiotic bacteria, with extracellular enzymes from the latter being transported to the gut. Previous research has predominantly focused on how these animals process the cellulose component of woody plant material, neglecting the breakdown of lignin - a tough, aromatic polymer which blocks access to the holocellulose components of wood. Enzymatic or non-enzymatic modification and depolymerization of lignin …