Life Sciences Commons^™

Assessing Perfectionism As A Predictor Of Exercise Dependency Over Time., Sarah E. Ernst

College of Arts & Sciences Senior Honors Theses

Exercise is generally thought to be beneficial for physical and mental health; however, when done in excess, exercise can lead to damaging physical, psychological, and social health consequences. This type of exercise is known as exercise dependency and is frequently associated with eating disorder pathology. Perfectionism (high standards and evaluative concerns) is a risk factor for eating disorders and is also associated with exercise dependency. However, no studies have examined longitudinal relationships between perfectionism and exercise dependency. The current study used a sample of adolescent females (N = 444) from a Southeastern United States high school, and participants completed …

Therapeutic Potential Of Withaferin A Against Non-Small Cell Lung Cancer., Al Hassan Kyakulaga

Electronic Theses and Dissertations

Lung cancer is the leading cause of cancer-related deaths worldwide and in the United States. Despite recent advancements in treatment approaches, chemoresistance and metastasis remain a major therapeutic challenge. Withaferin A (WFA), a plant-derived compound has recently emerged as a promising antitumor agent. In the present study, we explored the therapeutic potential of WFA against advanced NSCLC. Briefly, WFA was evaluated for its efficacy, toxicity and pharmacokinetic properties against advanced NSCLC using in vitro and in vivo models. Two human NSCLC cell lines, H1299 and A549 were used in cell culture to determine the anticancer properties of WFA. Repeat dose …

The Identification Of Long Non-Coding Rna Zfas1 Through An Exploratory Rna-Sequencing Analysis And Its Association With Epithelial-To-Mesenchymal Transition In Colon Cancer Adenocarcinoma., Stephen J. O'Brien

Electronic Theses and Dissertations

Colorectal adenocarcinoma is the fourth most common cancer diagnosed worldwide and is a significant cause of morbidity and mortality. This dissertation performed an exploratory RNA-sequencing analysis comparing gene expression between colon adenocarcinoma tissue and paired normal colon epithelium. After identification of a number of lncRNAs that were increased in expression in colon adenocarcinoma compared to normal colon epithelium, we aimed to validate the expression and investigate their function in vitro. Specifically, we focused on the lncRNA ZFAS1 and its association with epithelial-to-mesenchymal transition. These studies found the following: 1. Seven candidate lncRNAs were identified from the exploratory RNA-sequencing analysis to …

Understanding Object Motion Encoding In The Mammalian Retina., Victor Julian Depiero

Electronic Theses and Dissertations

Phototransduction, transmission of visual information down the optic nerve incurs delays on the order of 50 – 100ms. This implies that the neuronal representation of a moving object should lag behind the object’s actual position. However, studies have demonstrated that the visual system compensates for neuronal delays using a predictive mechanism called phase advancing, which shifts the population response toward the leading edge of a moving object’s retinal image. To understand how this compensation is achieved in the retina, I investigated cellular and synaptic mechanisms that drive phase advancing. I used three approaches, each testing phase advancing at a …

Investigation Of Phosphoserine Aminotransferase 1(Psat1) In Breast Cancer Progression., Stephanie Metcalf

Electronic Theses and Dissertations

This dissertation describes my research into the involvement of phosphoserine aminotransferase 1 (PSAT1) in breast cancer progression; specifically, in triple negative breast cancer (TNBC) metastasis and endocrine resistance in estrogen receptor positive breast cancer (ER+BC). Breast cancer is the most common tumor diagnosis among women. While the overall 5-year survival for breast cancer is reaching 90%, the 5-year survival for metastatic disease is only 22%. Metastasis and endocrine resistance combined can affect over 50% of patients. One of the proteins and pathways implicated in both metastasis and endocrine resistance in breast cancer is phosphoserine aminotransferase 1 (PSAT1) and the serine …

Characterizing The Roles Of Neutral Ceramidase In Cisplatin-Induced Kidney Injury., Sophia M. Sears

Electronic Theses and Dissertations

Cisplatin is a commonly used chemotherapeutic agent with a dose-limiting nephrotoxicity. 30% of patients given cisplatin develop acute kidney injury (AKI). AKI increases risk of chronic kidney disease (CKD) development and mortality. Patients that don’t develop clinical AKI are still at risk for long term declines in renal function. Currently, there are no FDA approved agents to treat or prevent cisplatin-induced kidney injury (CDDP-KI). In this study, we demonstrated that neutral ceramidase (nCDase) knockout provides protection from AKI in the high-dose model of CDDP-KI. However, in the repeated low dose cisplatin (RLDC) model of injury and we found nCDase knockout …

New Insights Into Anhydrobiosis Using Cellular Dielectrophoresis-Based Characterization, Mohamed Z. Rashed, Clinton J. Belott, Brett R. Janis, Michael Menze, Stuart J. Williams

Faculty Scholarship

Late embryogenesis abundant (LEA) proteins are found in desiccation-tolerant species from all domains of life. Despite several decades of investigation, the molecular mechanisms by which LEA proteins confer desiccation tolerance are still unclear. In this study, dielectrophoresis (DEP) was used to determine the electrical properties of Drosophila melanogaster (Kc167) cells ectopically expressing LEA proteins from the anhydrobiotic brine shrimp, Artemia franciscana. Dielectrophoresis-based characterization data demonstrate that the expression of two different LEA proteins, AfrLEA3m and AfrLEA6, increases cytoplasmic conductivity of Kc167 cells to a similar extent above control values. The impact on cytoplasmic conductivity was surprising, given …

Sustainable Solutions, Fall/Winter 2020, Issue 41

Sustain Magazine

No abstract provided.

Drinking Water Quality And Human Dimensions Of Cholera Patients To Inform Evidence-Based Prevention Investment In Karonga District, Malawi, Prince Kaponda, Suresh Muthukrishnan, Rory Barber, Rochelle H. Holm

Faculty Scholarship

Cholera remains a problem in sub-Saharan Africa, especially in Malawi. Our aim was to investigate drinking water source quality compared with water treatment, risk perception and cholera knowledge for patients who had reported to a health center for treatment in the 2017-2018 outbreak in Karonga District, Malawi. The study analyzed 120 drinking water samples linked to 236 cholera patients. Nearly 82% of the samples met the national criteria for thermotolerant coliforms of 50 cfu/100 ml, while 50% met the more stringent World Health Organization criteria of 0 cfu/100 ml. In terms of the human dimensions, 68% of survey respondents reported …

Letter From The Editor-In-Chief: Journal Of Refugee & Global Health Volume 2, Issue 2, Ruth Carrico

Journal of Refugee & Global Health

No abstract provided.

Implementing A Clinical Research Program In Long Term Care Facilities: Experiences From The University Of Louisville Center Excellence For Research In Infectious Diseases [Cerid], Ruth Carrico, Dawn Balcom, Kuldeep Ghosh, Bilal Abaid, Vidyulata Salunkhe, Bibodh Jung Karki, Simra Kiran, Stephen Furmanek, Senen Pena, Julio A. Ramirez

Journal of Refugee & Global Health

Background: According to the US Census Bureau International Report, in 2015, almost nine percent of the world’s population was aged 65 and over. As the worldwide population ages, there is a need to understand how to best care for those individuals. Developing clinical research programs focusing on long term care (LTC) will be critical to defining best practice.

Objectives: The objectives of this manuscript are to: 1) outline the challenges identified in performing clinical research in long term care facilities (LTCF), and 2) offer solutions for future clinical research in the LTC environment based upon our experiences.

Methods: A research …

Environmentally Responsible Land Use, Spring/Summer 2010, Issue 22

Sustain Magazine

No abstract provided.

Sustainable Communities, Fall/Winter 2010, Issue 21

Sustain Magazine

No abstract provided.

Sustainable Building, Spring/Summer 2009, Issue 20

Sustain Magazine

No abstract provided.

Environmental History, Fall/Winter 2009, Issue 19

Sustain Magazine

No abstract provided.

Student Research, Spring/Summer 2008, Issue 18

Sustain Magazine

No abstract provided.

Evolving Pollution Prevention, Fall/Winter 2008, Issue 17

Sustain Magazine

No abstract provided.

Climate Change, Spring/Summer 2007, Issue 16

Sustain Magazine

No abstract provided.

Our Energy Future, Fall/Winter 2007, Issue 15

Sustain Magazine

No abstract provided.

Land Conservation, Spring/Summer 2006, Issue 14

Sustain Magazine

No abstract provided.

Pollution And Heart Disease, Fall/Winter 2006, Issue 13

Sustain Magazine

No abstract provided.

Green Cities, Spring/Summer 2005, Issue 12

Sustain Magazine

No abstract provided.

Global Sustainability, Fall/Winter 2005, Issue 11

Sustain Magazine

No abstract provided.

Environmental Justice, Spring/Summer 2004, Issue 10

Sustain Magazine

No abstract provided.

Kentucky's Environmental Future, Fall/Winter 2004, Issue 9

Sustain Magazine

No abstract provided.

Urban Ecology, Spring/Summer 2003, Issue 8

Sustain Magazine

No abstract provided.

Urban Regeneration, Fall/Winter 2003, Issue 7

Sustain Magazine

No abstract provided.

Urban Air Quality, Spring/Summer 2002, Issue 6

Sustain Magazine

No abstract provided.

Sustainability Of Industrialized Agriculture, Fall/Winter 2002, Issue 5

Sustain Magazine

No abstract provided.

Urban Design For Environmental Protection, Spring/Summer 2001, Issue 4

Sustain Magazine

No abstract provided.