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Sesquiterpene Synthase Gene And Protein, Joe Chappell, Bryan T. Greenhagen
Sesquiterpene Synthase Gene And Protein, Joe Chappell, Bryan T. Greenhagen
Plant and Soil Sciences Faculty Patents
The invention relates to sesquiterpene synthases and methods for their production and use. Particularly, the invention provides nucleic acids comprising the nucleotide sequence of citrus valencene synthase (CVS) which codes for at least one CVS. The invention further provides nucleic acids comprising the nucleotide sequence coding for amino acid residues forming the tier 1 and tier 2 domains of CVS. The invention also provides for methods of making and using the nucleic acids and amino acids of the current invention.
Polypeptides, Nucleic Acid Molecules, And Methods For Synthesis Of Triterpenes, Joe Chappell, Thomas D. Niehaus, Shigeru Okada, Timothy P. Devarenne, David Watt
Polypeptides, Nucleic Acid Molecules, And Methods For Synthesis Of Triterpenes, Joe Chappell, Thomas D. Niehaus, Shigeru Okada, Timothy P. Devarenne, David Watt
Plant and Soil Sciences Faculty Patents
This application relates to the polypeptides, nucleic acid molecules, vectors, transfected cells, and methods for synthesis of triterpenes, including botryococcene.
Chimeric Isoprenoid Synthases And Uses Thereof, Joe Chappell, Kyoungwhan Back
Chimeric Isoprenoid Synthases And Uses Thereof, Joe Chappell, Kyoungwhan Back
Plant and Soil Sciences Faculty Patents
Provided is a chimeric isoprenoid synthase polypeptide including a first domain from a first isoprenoid synthase joined to a second domain from a second, heterologous, isoprenoid synthase, whereby the chimeric isoprenoid synthase is capable of catalyzing the production of isoprenoid reaction products that are not produced in the absence of the second domain of the second, heterologous, isoprenoid synthase. Also provided is a chimeric isoprenoid synthase polypeptide including an asymmetrically positioned heterologous domain, whereby the chimeric isoprenoid synthase is capable of catalyzing the production of isoprenoid reaction products that are not produced when the domain is positioned at its naturally-occurring …
Cytochrome P450s And Uses Thereof, Joe Chappell, Lyle F. Ralston
Cytochrome P450s And Uses Thereof, Joe Chappell, Lyle F. Ralston
Plant and Soil Sciences Faculty Patents
The invention features isolated cytochrome P450 polypeptides and nucleic acid molecules, as well as expression vectors and transgenic plants containing these molecules. In addition, the invention features uses of such molecules in methods of increasing the level of resistance against a disease caused by a plant pathogen in a transgenic plant, in methods for producing altered compounds, for example, hydroxylated compounds, and in methods of producing isoprenoid compounds.
B. Braunii, Race B Gene For A Triterpene Methyltransferase Enzyme And Uses Thereof, Joe Chappell, Okada Shigeru, Scott Kinison, Tom Niehaus
B. Braunii, Race B Gene For A Triterpene Methyltransferase Enzyme And Uses Thereof, Joe Chappell, Okada Shigeru, Scott Kinison, Tom Niehaus
Plant and Soil Sciences Faculty Patents
Provided is an isolated polypeptide having triterpene methyltransferase activity. Also provided is an isolated nucleic acid molecule that encodes the triterpene methyltransferase polypeptides; a vector comprising the nucleic acid molecules that encode the triterpene methyltransferase polypeptides; and a host cell(s) transfected with the aforementioned nucleic acid molecule or vector. In another aspect, a method of producing a methylated triterpene is provided. The method comprises providing a metabolizable carbon source to a host cell transfected with a nucleic acid molecule that encodes a triterpene methyltransferase under conditions sufficient for production of a methylated triterpene. The method optionally further comprises isolating the …