Open Access. Powered by Scholars. Published by Universities.®
- Keyword
Articles 1 - 4 of 4
Full-Text Articles in Life Sciences
Tall Fescue Seed Extraction And Partial Purification Of Ergot Alkaloids, Huihua Ji, Franklin Fannin, J. Klotz, Lowell P. Bush
Tall Fescue Seed Extraction And Partial Purification Of Ergot Alkaloids, Huihua Ji, Franklin Fannin, J. Klotz, Lowell P. Bush
Kentucky Tobacco Research and Development Center Faculty Publications
Many substances in the tall fescue/endophyte association (Schedonorus arundinaceus/Epichloë coenophiala) have biological activity. Of these compounds only the ergot alkaloids are known to have significant mammalian toxicity and the predominant ergot alkaloids are ergovaline and ergovalinine. Because synthetically produced ergovaline is difficult to obtain, we developed a seed extraction and partial purification protocol for ergovaline/ergovalinine that provided a biologically active product. Tall fescue seed was ground and packed into several different sized columns for liquid extraction. Smaller particle size and increased extraction time increased efficiency of extraction. Our largest column was a 114 × 52 × …
An Overview Of The Gene Regulatory Network Controlling Trichome Development In The Model Plant, Arabidopsis, Sitakanta Pattanaik, Barunava Patra, Sanjay Kumar Singh, Ling Yuan
An Overview Of The Gene Regulatory Network Controlling Trichome Development In The Model Plant, Arabidopsis, Sitakanta Pattanaik, Barunava Patra, Sanjay Kumar Singh, Ling Yuan
Kentucky Tobacco Research and Development Center Faculty Publications
Trichomes are specialized epidermal cells located on aerial parts of plants and are associated with a wide array of biological processes. Trichomes protect plants from adverse conditions including UV light and herbivore attack and are also an important source of a number of phytochemicals. The simple unicellular trichomes of Arabidopsis serve as an excellent model to study molecular mechanism of cell differentiation and pattern formation in plants. The emerging picture suggests that the developmental process is controlled by a transcriptional network involving three major groups of transcription factors (TFs): the R2R3 MYB, basic helix-loop-helix (bHLH), and WD40 repeat (WDR) protein. …
Psim24 Is A Novel Versatile Gene Expression Vector For Transient Assays As Well As Stable Expression Of Foreign Genes In Plants, Dipak K. Sahoo, Nrisingha Dey, Indu B. Maiti
Psim24 Is A Novel Versatile Gene Expression Vector For Transient Assays As Well As Stable Expression Of Foreign Genes In Plants, Dipak K. Sahoo, Nrisingha Dey, Indu B. Maiti
Kentucky Tobacco Research and Development Center Faculty Publications
We have constructed a small and highly efficient binary Ti vector pSiM24 for plant transformation with maximum efficacy. In the pSiM24 vector, the size of the backbone of the early binary vector pKYLXM24 (GenBank Accession No. HM036220; a derivative of pKYLX71) was reduced from 12.8 kb to 7.1 kb. The binary vector pSiM24 is composed of the following genetic elements: left and right T-DNA borders, a modified full-length transcript promoter (M24) of Mirabilis mosaic virus with duplicated enhancer domains, three multiple cloning sites, a 3'rbcsE9 terminator, replication functions for Escherichia coli (ColE1) and Agrobacterium tumefaciens (pRK2-OriV) and the replicase trfA …
Overexpression Of The Synthetic Chimeric Native-T-Phylloplanin-Gfp Genes Optimized For Monocot And Dicot Plants Renders Enhanced Resistance To Blue Mold Disease In Tobacco (N. Tabacum L.), Dipak K. Sahoo, Sumita Raha, James T. Hall, Indu B. Maiti
Overexpression Of The Synthetic Chimeric Native-T-Phylloplanin-Gfp Genes Optimized For Monocot And Dicot Plants Renders Enhanced Resistance To Blue Mold Disease In Tobacco (N. Tabacum L.), Dipak K. Sahoo, Sumita Raha, James T. Hall, Indu B. Maiti
Kentucky Tobacco Research and Development Center Faculty Publications
To enhance the natural plant resistance and to evaluate the antimicrobial properties of phylloplanin against blue mold, we have expressed a synthetic chimeric native-phylloplanin-GFP protein fusion in transgenic Nicotiana tabacum cv. KY14, a cultivar that is highly susceptible to infection by Peronospora tabacina. The coding sequence of the tobacco phylloplanin gene along with its native signal peptide was fused with GFP at the carboxy terminus. The synthetic chimeric gene (native-phylloplanin-GFP) was placed between the modified Mirabilis mosaic virus full-length transcript promoter with duplicated enhancer domains and the terminator sequence from the rbcSE9 gene. The chimeric gene, expressed in transgenic …