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Induced Spawning Of Captive Tripletail Lobotes Surinamensis, Nicholas Adams
Induced Spawning Of Captive Tripletail Lobotes Surinamensis, Nicholas Adams
Master's Theses
The Tripletail, Lobotes surinamensis, is an emerging candidate species for U.S. marine aquaculture. This work aimed to address two bottlenecks for hatchery production of the species by developing a hormonal induction protocol to obtain fertile spawns from captive brooders and a method for sex identification of candidate brooders.
Single pairs selected among a captive-held broodstock conditioned under a natural photothermal cycle were induced with one of five treatments (n = 5 or 6 replicates per treatment). Control (no hormone) and hCG (1,100 IU.kg-1 for females, 550 IU.kg-1 for males) pairs did not spawn. Pairs treated with GnRHa …
Sensitivity Of A Lamp Assay For Detection Of The Dinoflagellate Amyloodinium Ocellatum In Simulated Field Conditions And Freeze Tolerance Of The Parasite, Robert Gonzales
Sensitivity Of A Lamp Assay For Detection Of The Dinoflagellate Amyloodinium Ocellatum In Simulated Field Conditions And Freeze Tolerance Of The Parasite, Robert Gonzales
Master's Theses
The obligate parasitic dinoflagellate Amyloodinium ocellatum causes amyloodiniosis in warm water marine fishes. The prolific parasite, which has a direct, three-stage life cycle, is highly infectious and can cause heavy losses in aquaculture. Prevention, biosecurity, and early detection are vital for control. In this work, microscopy and a loop-mediated isothermal amplification (LAMP) assay were compared for early diagnosis of A. ocellatum in cultured stocks, and the freeze tolerance of tomonts was assessed to determine if frozen wild fish used as fish food can serve as a potential vector for the parasite.
The lowest dinospore concentration that could be detected by …