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Full-Text Articles in Life Sciences

Gut Microbiota Of Migratory Passerines At Stopover, William Bagley Lewis Dec 2015

Gut Microbiota Of Migratory Passerines At Stopover, William Bagley Lewis

Master's Theses

Although the gut microbiota provides many beneficial functions to animal hosts, relatively little is known about the gut microbiota of passerines. It is likely that gut microbes are especially important during the migratory phase; however gut atrophy experienced during prolonged migratory flight may cause disruptions of the stable microbiota. Fecal samples were collected from several species of passerine after crossing the Gulf of Mexico during spring migration and before crossing during fall, and microbiota communities were analyzed using next-generation sequencing. Despite showing large inter-individual variation, a core microbiota composed largely of Firmicutes and Proteobacteria was identified in all birds. Microbiota …


The Vascular Flora Of Wayne County, Mississippi, Daniel M. Mcnair Dec 2015

The Vascular Flora Of Wayne County, Mississippi, Daniel M. Mcnair

Master's Theses

The vascular flora of Wayne County, Mississippi, was surveyed from 2013–2015, and the species of native and naturalized plants are listed. In total, 1,135 species were identified from field collections, previously collected herbarium specimens, and field observations. These belong to 556 genera and 165 families. Four of these species represent first recorded occurrences in the state of Mississippi: Blyxa aubertii Rich., Gomphrena serrata Pav. ex Moq., Isoetes valida (Engelm.) Clute, and Polypogon interruptus Kunth. GPS coordinates are given for important collection sites representing the diversity of plant communities in the county. All voucher specimens from the survey are deposited in …


A Platform For Fast Detection Of Let-7 Micro Rna Using Polyaniline Fluorescence And Image Analysis Techniques, Partha P. Sengupta Dec 2015

A Platform For Fast Detection Of Let-7 Micro Rna Using Polyaniline Fluorescence And Image Analysis Techniques, Partha P. Sengupta

Master's Theses

The project describes a new strategy for transducing hybridization events through modulating intrinsic properties of the electroconductive polymer polyaniline (PANI). When DNA based probes electrostatically interact with PANI, its fluorescence properties are increased, a phenomenon that can be enhanced by UV irradiation. Hybridization of target nucleic acids results in dissociation of probes causing PANI fluorescence to return to basal levels. By monitoring restoration of base PANI fluorescence as little as 10-11 M (10 pM) of target oligonucleotides could be detected within 15 minutes of hybridization. Detection of complementary oligos was specific, with introduction of a single mismatch failing to …


Same-Sex Socio-Sexual Interactions Among A Group Of Captive Bottlenose Dolphins (Tursiops Truncatus), Natalia Botero Acosta Dec 2015

Same-Sex Socio-Sexual Interactions Among A Group Of Captive Bottlenose Dolphins (Tursiops Truncatus), Natalia Botero Acosta

Master's Theses

Bottlenose dolphins (Tursiops truncatus) frequently engage in non-reproductive sexual behavior, including homosexual encounters. In order to better understand the nature and function of these interactions, a longitudinal study of the patterns of association and the dynamics of initiator/recipient role exchange was conducted. Underwater video footage of a colony of bottlenose dolphins housed at the Roatan Institute for Marine Sciences (RIMS), collected between March of 2010 and May of 2013, was analyzed. Associations occurring during homosexual interactions were transitory for most individuals. Nonetheless, subsequent analyses allowed the rejection of the null hypothesis of random association, suggesting the existence of …


Maternal Effects And Offspring Behavior: Potential Contributors To The Lack Of Recruitment In Mississippi Gopher Tortoises (Gopherus Polyphemus), Aaron L. Holbrook Dec 2015

Maternal Effects And Offspring Behavior: Potential Contributors To The Lack Of Recruitment In Mississippi Gopher Tortoises (Gopherus Polyphemus), Aaron L. Holbrook

Master's Theses

Federally threatened in Mississippi, gopher tortoise (Gopherus polyphemus) populations within the state have strongly variable recruitment and are generally in decline. Hatching success is significantly lower in Mississippi than in any other part of the species’ range , and most hatchlings die within the first year. There are few refuges where survival and hatching success is high. Here I compare two populations that differ in recruitment and offspring survival for differences in corticosterone. Corticosterone is a hormone that influences both energy availability and is released in elevated levels during stressful events, like living in poor quality habitat. Prolonged corticosterone elevation …


High Resolution Melt Analysis Of Samples With Differential Dna Methylation To Identify Tissue Source Of Origin, Stephanie M. Ledgerwood Dec 2015

High Resolution Melt Analysis Of Samples With Differential Dna Methylation To Identify Tissue Source Of Origin, Stephanie M. Ledgerwood

Master's Theses

In Forensic Science casework, identifying the source of a tissue can assist in crime scene reconstruction. Currently, presumptive testing methods to identify tissue type are utilized in crime scene laboratories, but there is a need for a more reliable confirmatory test for tissue type identification. High Resolution Melt (HRM) analysis is an innovative technology that has the potential to determine tissue types through variations in DNA methylation patterns. Recently, DNA methylation patterns have been found to correspond with specific tissue types in particular regions of DNA. Two markers, B_SPTB_03 and DDX4 have been effective in differentiating sperm from other tissue …


Territorial Defense Strategies In The Northern Cardinal (Cardinalis Cardinalis): Who Is The Bigger Threat?, Kaylee M. Gentry Dec 2015

Territorial Defense Strategies In The Northern Cardinal (Cardinalis Cardinalis): Who Is The Bigger Threat?, Kaylee M. Gentry

Master's Theses

This thesis examines the use of defensive strategies in relation to territories year round in the northern cardinal (Cardinalis cardinalis). Responses to recorded neighbor song and stranger song playback from the middle of a focal male’s territory were measured. This allowed for an estimation of aggression in both the winter and spring seasons. Each focal male was subjected to both treatments (stranger song and neighbor song). Males were more responsive over-all to neighbor song playback, however in the winter months, persistence of response to neighbor song playback increased. It was also shown that southeastern United States cardinals show …


Are Large Dogs Smarter Than Small Dogs? Investigating Within Species Differences In Large And Small Dogs: Spatial Memory, Megan S. Broadway Dec 2015

Are Large Dogs Smarter Than Small Dogs? Investigating Within Species Differences In Large And Small Dogs: Spatial Memory, Megan S. Broadway

Master's Theses

The study of canine cognition can be useful in understanding the ontology and selective pressures that affect the development of cognitive abilities. Dogs have undergone intensive artificial selection yielding distinctive breeds which differ both phenotypically and behaviorally. Breed based cognitive differences have not been found but some studies suggest there may be differences in broader categories such as working disposition and sex. The influence of size on canine cognition has not been thoroughly addressed despite the fact that large dogs are often perceived to be ‘smarter’ than small dogs. This preconception has only recently been addressed and supported in one …


Management Strategy Evaluation For The Atlantic Surfclam, Spisula Solidissima, Using A Fisheries Economics Model, Kelsey M. Kuykendall Dec 2015

Management Strategy Evaluation For The Atlantic Surfclam, Spisula Solidissima, Using A Fisheries Economics Model, Kelsey M. Kuykendall

Master's Theses

The Atlantic surfclam, Spisula solidissima, is an economically valuable bivalve harvested along the northeastern United States. The surfclam’s range has contracted and the center of the stock’s distribution has shifted north driven by warmer bottom water temperatures. Declining landings per unit effort (LPUE) in the Mid-Atlantic Bight (MAB) is one result. Declining stock abundance and LPUE suggest that overfishing may be occurring off New Jersey. The objective of this project is to perform a management strategy evaluation (MSE) for Spisula solidissima. The terminal goal is to identify a preferred management option that promotes enhanced surfclam productivity in the …


Latitudinal Variation In Thermal Tolerance And Preference Of Fundulus Notatus And F. Olivaceus: Implications For Temperature Mediated Reproductive Isolation, Laura Kristin Stewart Aug 2015

Latitudinal Variation In Thermal Tolerance And Preference Of Fundulus Notatus And F. Olivaceus: Implications For Temperature Mediated Reproductive Isolation, Laura Kristin Stewart

Master's Theses

Temperature is one of the most pervasive and influential abiotic factors affecting the life histories of fish, regulating a host of physiological processes and influencing behavioral thermoregulation. Fundulus notatus, the blackstripe topminnow, and F. olivaceus, the blackspotted topminnow, are widely distributed species located in drainages along the northern part of Gulf of Mexico (30°N latitude) and as far north as southern Illinois (39°N latitude). However, F. notatus has a much broader range reaching up into the Great Lakes (43°N latitude). Due to their broad geographical range, these populations inhabit a wide range of thermal regimes, thus …


Habitat Selection Of Gulf-Strain Striped Bass, Morone Saxatilis: Relationships To Dynamic Abiotic Environmental Characteristics Within The Biloxi River, Mississippi, Jennifer Lynne Green Aug 2015

Habitat Selection Of Gulf-Strain Striped Bass, Morone Saxatilis: Relationships To Dynamic Abiotic Environmental Characteristics Within The Biloxi River, Mississippi, Jennifer Lynne Green

Master's Theses

The purpose of this project was to provide insights about the short- and long-term patterns of habitat selection of Gulf-strain Striped Bass, Morone saxatilis, based on spatially and seasonally variable abiotic environmental characteristics (water temperature, dissolved oxygen (DO), salinity, specific conductivity, and pH) in the Biloxi River, MS system. Juvenile hatchery-reared and feral adult Gulf-strain Striped Bass were acoustically-tagged and continuously monitored via active and passive telemetry from November 2012 – June 2014. Each month the available abiotic environmental characteristics of 40 random locations within the Biloxi River, along with sub-habitat conditions occupied by acoustically-tagged Gulf-strain Striped Bass, were …


Naturalists’ Perspectives On The Use Of Mobile Technology During A Nature Hike, Aubin Marishka Radzewicz St. Clair Aug 2015

Naturalists’ Perspectives On The Use Of Mobile Technology During A Nature Hike, Aubin Marishka Radzewicz St. Clair

Master's Theses

Naturalists act as our link between scientific knowledge and the public’s understanding of natural history and conservation efforts. In order for them to succeed, they need access to reference materials as well as up-to-date information (Mankin, Warner, & Anderson, 1999). Incorporating mobile technology (i.e. tablets) into naturalists’ endeavors in natural history and environmental education can be used as supportive and educational tools. My project investigated how newly trained naturalists used tablet technology while leading groups of children on nature hikes. I investigated naturalists’ views on the use of mobile technology as a tool during the hikes. My research was guided …


Evaluation Of The Protein Recognition Properties Of Peptide Nucleic Acids, Crystal Cox Serrano Aug 2015

Evaluation Of The Protein Recognition Properties Of Peptide Nucleic Acids, Crystal Cox Serrano

Master's Theses

The objective is to evaluate the ability of aminoacyl-tRNA synthetases (aaRS) to recognize the non-standard nucleic acid, PNA (peptide nucleic acid). PNA has immense potential in biomedical applications due to its increased thermostability and nuclease resistance over natural nucleic acids. PNA represents a superior alternative to natural nucleic acids in many biomedical applications due to its specificity, strong binding, and nuclease resistance. This study is the initial data set that indicates PNA are recognized by translation enzymes.


Elucidating The Molecular Function Of Reprolysin Metalloproteases In Tick-Host-Pathogen Interaction, Jaclyn Bo Williams Aug 2015

Elucidating The Molecular Function Of Reprolysin Metalloproteases In Tick-Host-Pathogen Interaction, Jaclyn Bo Williams

Master's Theses

Ticks are ectoparasites that attach to their host for many days to weeks, feeding to engorgement and continuing their species life cycle. The mechanisms behind the successful feeding and manipulation of host immune responses have been associated with the secretion of their specialized saliva. Tick saliva includes a variety of modulatory molecules that must disrupt and counteract defense mechanisms, including a variety of proteases. Reprolysin Metalloproteases, in the Zn2+ dependent family, is secreted as a proenzyme and is shown to have proteolytic activity, degrading fibrinogen and gelatinase activity, as well as disturbing homeostasis12. Previous work with …


Ichthyoplankton Composition In The Loop Current And Sargassum Habitats In The Northern Gulf Of Mexico, Stephanie Morgan Taylor Aug 2015

Ichthyoplankton Composition In The Loop Current And Sargassum Habitats In The Northern Gulf Of Mexico, Stephanie Morgan Taylor

Master's Theses

Ichthyoplankton in the Gulf of Mexico (GOM) aggregate in two different habitats that serve to provide refuge and concentrate prey in an otherwise oligotrophic environment: the Loop Current (LC) a mesoscale oceanographic feature characterized by the transition of environmental conditions of the water mass between the GOM and Caribbean; and Sargassum, a two species assemblage of floating brown algae that is habitat for many larval and juvenile obligate and associated fishes. The objectives of this study were to characterize the density and community composition of ichthyoplankton within these habitats using multivariate analysis. I identified three unique assemblages of ichthyoplankton …


Analyses Of Indices Of Abundance For Important Groundfish Species In The Northern Gulf Of Mexico From 1987-2009, Relative To Shrimp Bycatch; With Age And Growth Of Three Sciaenid Species, Allison Renee Odom Aug 2015

Analyses Of Indices Of Abundance For Important Groundfish Species In The Northern Gulf Of Mexico From 1987-2009, Relative To Shrimp Bycatch; With Age And Growth Of Three Sciaenid Species, Allison Renee Odom

Master's Theses

Bycatch in the shrimp fishery became of particular concern in the 1980s during the peak of shrimp harvest in the northern Gulf of Mexico. Shrimp bycatch is of major importance because the majority of catch in the fishery is not intended target species of commercial shrimp, and most of the additional bycatch is returned to the sea dead or dying, inducing higher rates of mortality. This study was developed to assess the potential ecosystem effects of shrimp harvest on thirty selected species/species groups commonly encountered within the shrimp fishery as bycatch. Delta-lognormal modeling (DLM) was used to determine the relative …


Observations On The Life History Of The Bayou Killifish, Fundulus Pulvereus (Everman 1899) In Mississippi Tidal Marshes, Leah Corrine Gaines May 2015

Observations On The Life History Of The Bayou Killifish, Fundulus Pulvereus (Everman 1899) In Mississippi Tidal Marshes, Leah Corrine Gaines

Master's Theses

This study provides insight into the life history of the relatively unknown intertidal marsh fish Fundulus pulvereus (Everman), the bayou killifish. Monthly sampling for F. pulvereus took place in three different Mississippi tidal marshes over a period of 17 months (n=445). Observations on the diet, parasite fauna, and fecundity of the bayou killifish were documented on all specimens collected. Percent Occurrence and Percent Number coupled along with multivariate analyses indicated seasonal, site, and ontogenetic shifts in the diet of F. pulvereus. Dominant prey items consumed by F. pulvereus were copepods, mites, chironomids, and terrestrial and aquatic insects. The bayou …


Seasonal Variation In Corticosterone, Stress Responses, And Physiology In The Northern Cardinal Cardinalis Cardinalis, Benjamin Matthew Duckworth May 2015

Seasonal Variation In Corticosterone, Stress Responses, And Physiology In The Northern Cardinal Cardinalis Cardinalis, Benjamin Matthew Duckworth

Master's Theses

Seasonal regulation of the adrenocortical response (e.g., ‘stress response’) appears to be ubiquitous in mid- to high-latitude vertebrates. Northern Cardinals (Cardinalis cardinalis) are a Temperate dwelling passerine of Tropical-descent with a wide species range (tropics to Northern Temperate zones). This species encounters a wide variety of environmental conditions and strong seasonality, however corticosterone (CORT, the main adrenal stress hormone) has not been studied with regards to changes in seasonal levels. I initially analyzed samples from cardinals collected between 2007-2011 from the Lake Thoreau Environmental Research and Educational Center (Hattiesburg, Mississippi, USA). The data suggested seasonal differences of CORT, with higher …


Effects Of Pre-Release Physical Stressors On Post-Release Success Of Hatchery-Reared Spotted Seatrout, Taylor Westbrook Guest May 2015

Effects Of Pre-Release Physical Stressors On Post-Release Success Of Hatchery-Reared Spotted Seatrout, Taylor Westbrook Guest

Master's Theses

Alteration of habitat associated with coastal development and increased demand for food and recreation can result in the depletion of fisheries resources such as the Spotted Seatrout (Cynoscion nebulosus), the Gulf of Mexico’s most popular recreational fish. Stock enhancement, or the release of cultured fish to supplement wild populations, is one potential tool for managing important fisheries resources. Although much effort has gone into marine stock enhancement, the effectiveness of stocking is not well established, and techniques for ensuring success have not been developed. Although the basic biology of the Spotted Seatrout is well known, comparatively little is known about …


Stressed Induced Changes In Karenia Brevis Ribosomal Rna, David Scott Jayroe May 2015

Stressed Induced Changes In Karenia Brevis Ribosomal Rna, David Scott Jayroe

Master's Theses

Karenia brevis is a toxic marine dinoflagellate that causes harmful algal blooms (HABs), also known as red tides, in the Gulf of Mexico. These blooms are responsible for massive fish kills, shellfish bed contaminations, adverse human health effects, and vast economic loss. For these reasons, extensive research has gone into understanding the mechanisms and dynamics of bloom behavior by studying K. brevis in the field and in the lab. In order to understand higher order bloom behavior and dynamics it is imperative to understand K. brevis at the cellular level. In growing K. brevis in vitro under a variety of …


Impacts Of The Deepwater Horizon Oil Spill On Microbial-Mediated Cellulose Decomposition In Mississippi Gulf Coast Salt Marshes, Jerrid Shawn Boyette May 2015

Impacts Of The Deepwater Horizon Oil Spill On Microbial-Mediated Cellulose Decomposition In Mississippi Gulf Coast Salt Marshes, Jerrid Shawn Boyette

Master's Theses

Field studies were conducted to examine the effects of the Deepwater Horizon oil spill on rates of marsh organic matter decomposition. Decomposition in surface and subsurface marsh sediments was assessed in stands of Spartina alterniflora and Juncus roemerianus in 9 Mississippi Gulf Coast marshes exposed to differing oiling intensities. The cotton strip bioassay technique was used as a proxy for cellulose decomposition. In addition, rates of microbial respiration, fungal biomass (ergosterol) and nutrients (C:N, C:P) of surface sediment cotton strips were also quantified. Subsurface cotton strip decay, as determined by losses in tensile strength, were significantly different among marsh …


Deciphering The Functional Collaboration Of Mid And Bric-A-Brac 2 As Potential Regulators Of Cellular Proliferation Within Adult Drosophila Ovaries, Petra Visic May 2015

Deciphering The Functional Collaboration Of Mid And Bric-A-Brac 2 As Potential Regulators Of Cellular Proliferation Within Adult Drosophila Ovaries, Petra Visic

Master's Theses

Stem cell niches are highly organized and specialized microenvironments located within specific tissues of both vertebrate and invertebrate organisms [1]. In Drosophila melanogaster, three distinct stem cell niches have been identified within the ovary including the germline stem cell (GSC), follicle stem cell (FSC), and escort stem cell (ESC) niche. Recently, Fregoso-Lomas et al. [2] reported that Gurken/Epidermal Growth Factor Receptor (EGFR) signaling is modulated within posterior ovarian follicle cells by Midline (Mid). The mid gene encodes a T-box transcription factor protein that specifies cell fates in the developing heart [3][4], central nervous system [5][6], epidermis [7], and eye …