Life Sciences Commons^™

Impact Of Temperature On Children’S Nutrition: A Comparative Study Of Three Ecological Regions Of Nepal, Prakriti Shakya

Master's Theses

Nutrition obtained during the growth period of childhood significantly influences long-term well-being and overall productivity, ultimately contributing to the economy of a society. However, weather shocks can wreak havoc by damaging crops, changing yields of important crops and disrupting market access, which directly impacts the food intake of both adults and children. When these adverse events occur during childhood, short term and long term inadequacy in nutrition as well as disease incidence can cause malnutrition leading to stunted growth and cognitive impairment that may persist into adulthood, affecting the labor market and increasing health expenditure. To address this issue, we …

The Impact Of Index Based Livestock Insurance (Ibli) On Child Nutrition In Marsabit County, Kenya, Jackson Kadyampakeni

Master's Theses

The study uses six rounds of Index-Based Livestock Insurance (IBLI) panel surveys (2009 – 2015) for Northern Kenya’s Marsabit county to investigate the impact of IBLI on child nutrition and household food security. We employ Ordinary Least Squares (OLS) and Instrumental Variable (IV) regressions to account for potential endogeneity in IBLI uptake. The results indicate that while IBLI uptake significantly improves the intake of key nutrients such as Vitamin A, protein, iron, and fruits and vegetables, it does not translate into significant improvements in child nutrition status, as measured by Mid-Upper Arm Circumference (MUAC) z-scores. Significant negative effects of age …

Pfas Exposure And Human Health Risk Management - A Policy Review, Callie Sierra Totaro

Master's Projects and Capstones

For centuries, new chemicals and compounds have been invented to improve material longevity or make a product cheaper to produce. Throughout this time, emerging chemicals have often gone through inadequate testing that may not consider all long term environmental and human health effects before they have gone to market in the United States. As a result, thousands of harmful chemicals have ended up in the food supply, water sources, and everyday items of people in the United States with the negative health effects often not being discovered for decades after the start of circulation. By this point, removal and assessment …

Assessing The Use Of Regenerative Agriculture In California Almonds As Climate Change Resilience, Skyler M. Seamons

Master's Projects and Capstones

The agriculture sector is responsible for 10% of the United States’ greenhouse gas emissions. In turn, anthropogenic climate change threatens crops. With its Mediterranean climate, California is the country’s largest agricultural-producing state. Many California crops are at risk due to increasing temperatures and changed precipitation patterns. This paper investigates regenerative farming techniques as a tool to protect California crops from a changing climate. Almonds are used as a case study to analyze the soil management practices, finances, and policies underlying regenerative agriculture in California. A literature review and comparative analysis are used to compare regenerative and conventional soil management practices …

Managing The Environmental And Socio-Economic Impacts Of Rohingya Refugees In Bangladesh, Mousume Azad

Master's Projects and Capstones

In August 2017, nearly 1 million Rohingya people fled to Bangladesh to save their lives from the genocide inflicted by the military of Myanmar. At present, over 1.3 million Rohingyas are staying at Cox’s Bazar, Bangladesh, in 34 overcrowded refugee camps. This research examined the environmental impacts especially the change in vegetation cover and land surface temperature as well as the socioeconomic alteration of the host country after the refugee influx. The research found a 5488 ha or 9.58% decrease in forest area, accompanied by an 8.25% increase in refugee settlement areas, an increase in average land surface temperature within …

From Pixels To Plants: Remote Sensing Of California Invasive Plants, Kenneth Rangel

Master's Projects and Capstones

Invasive plants cause significant impacts to ecosystems, the economy, and human health. California has experienced significant plant invasions and is well suited to future invasion because of its Mediterranean climate and human disturbance. Eradication or control of invasive plant species requires a detailed understanding of their spatial distribution, which typically involves on the ground surveys that can be expensive or inconsistent. Remote sensing offers a potential alternative or supplement to in-person invasive plant mapping. This study performed a comparative analysis of 41 remote sensing studies that mapped the distribution of California invasive plants. I found that while high spectral resolution …

Investigating The Modulation Of Metastasis By Dax-1 In Adrenal Carcinoma Cells, Aarya Mishra

Undergraduate Honors Theses

The nuclear hormone receptor (NHR), DAX-1 (dosage-sensitive sex reversal, adrenal hypoplasia critical region, on chromosome X, gene 1), is important in adrenal and gonadal development as well as steroidogenesis. It is encoded by the NR0B1 gene and functions mainly as a transcriptional repressor. Classified as an orphan receptor within the NHR superfamily, DAX-1 has been shown to inhibit other NHRs including estrogen receptor, androgen receptor and steroidogenic factor 1. DAX-1 is found to be underexpressed in breast and prostate cancers and, specifically in prostate cancer, is believed to be a transcriptional repressor of genes that are involved in epithelial-mesenchymal transition …

Role Of Toll-Like Receptor 2 In Macrophage Recognition And Response To Borrelia Burgdorferi, Yukiye A. Koide

Master's Theses

Lyme disease is the most common vector-borne disease in the US and will experience an uptick as the insect host, the ixodid tick, gains habitat with climate change. The causative agent of Lyme disease, Borrelia burgdorferi, is recognized by Toll-like receptor 2 (TLR2), which initiates the innate immune response. Here, I used ELISA and SEAP detection assays to determine that knocking out TLR2 in THP-1 macrophages reduces the amount of NFkB activation, as well as IL-10 and IL-1β secretion. Then, I used fluorescence microscopy and Incucyte assays to quantify the amount of phagocytosis performed by wild-type and knock-out THP-1 cells. …

Covalent Inhibition Of Enzyme Sortase A As A New Pathway Against Bacterial Resistance Of Staphylococcus Aureus, Umyeena Bashir

Master's Theses

The continued rise of antibiotic-resistant bacteria, such as MRSA (methicillin-resistant Staphylococcus aureus), has made the discovery of novel antibiotics critical. While bacteriostatic and bactericidal antibiotics inevitably exert evolutionary pressure on bacteria to develop resistance, small molecules that target mechanisms of bacterial virulence present a promising alternative for treatment. Sortase A (Srt A), a cysteine protease of S. aureus, promotes bacterial virulence by covalently attaching proteins such as pilin to the bacterial surface, enabling bacterial adhesion to mammalian cells. Inhibitory studies of this enzyme have gained prominent interest as a new pathway for drug development since blocking this enzyme's ability to …

Nanodiscs: A Novel Approach To The Study Of The Methionine Abc Transporter System, Michael T. Winslow

Master's Theses

Membrane transporter proteins play the vital role of moving compounds in and out of the cell and are essential for all living organisms. ATP Binding Cassette (ABC) family transporters function both as importers and exporters in prokaryotes. MetNI is an E. coli Type I ABC transporter responsible for the uptake of methionine into the cytosol from the cell periplasmic space through the cell membrane to maintain intracellular methionine pools. ABC transporters, like other membrane proteins, are most often mechanistically and structurally studied in vitro, solubilized by detergents. However, detergent micelles may affect the conformational changes of membrane proteins relative to …

Improving The Land Trust Model’S Impact On Environmental Conservation In Northern California, Peter Talbot

Master's Projects and Capstones

For years, the land trust sector of California and much of the United States has operated with a dollars and acres mentality that has prioritized fundraising as a result of acreage protected. Within California, nearly 5.8 million acres of land have been protected by 132 land trusts throughout the state. To accommodate for the diverse cross-section of land and the many needs of the population, land trusts take on numerous shapes and sizes. A unique aspect of this diversity is the rich agricultural and natural spaces found throughout the state. This mix of land and variety of land uses has …

Considerations For The Use Of Mycorrhizal Fungi Inoculants In Coal Mine Reclamation And Reforestation In Appalachia: A Guide Of Best Practices And Management Recommendations, Cole Hansen Reistrup

Master's Projects and Capstones

Nearly all terrestrial plants form symbiotic relationships with mycorrhizal fungi. These beneficial soil microbes support plant health by increasing plants’ access to nutrients and water, protecting them from pathogens, improving soil properties, and more. A challenge to restoring forests on coal mined land in Appalachia is that mine soils are often degraded in a number of ways, including lacking mycorrhizal fungi, which makes mine soils difficult for trees to grow upon. Substantial improvements to coal mine reforestation success have been made since the development of the Forestry Reclamation Approach in 2005 that provides guidelines for how to create a suitable …

Desalination: Adapting To A Changing Climate And An Increasing Demand For Freshwater, Rebecca A. Acosta

Master's Projects and Capstones

The state of California is just one place in the world that is experiencing an increasing demand for freshwater while also experiencing increasingly hotter conditions and longer periods of drought. There are a number of plants slated for development in the state of California but have been met with resistance from the public with concerns regarding their impacts to the surrounding marine environments. This paper provides background on desalination plants, provides evidence for a potential indicator species, identifies potential impacts to marine environments, and addresses stakeholder concerns and perceptions around desalination plants.

Using Critical Incident Debriefing After Code Blue Events To Support Registered Nurses, David L. Boyd

Doctor of Nursing Practice (DNP) Projects

Using Critical Incident Debriefing after Code Blue Events to Support Registered Nurses

Section I: Abstract

Background: During a code blue event (CBE), the environment becomes highly stressful and intense. Nurses rapidly transition from performing life-saving procedures on their patients to carrying out their usual duties. Without proper debriefing, nurses cannot properly process their emotions leading to increased burnout and secondary traumatic stress (Stamm, 2010).

Local Problem: In 2021, the nurses at Providence Saint John’s Health Center (2021a) responded to 110 CBEs, a 43% increase from 2020. Without a process for critical incident debriefing (CID), these nurses were exposed to trauma …

Investigating The Role Of Tgf-Beta Signaling In Acoel Regeneration & Fission, Franchesca A. Aziz

Undergraduate Honors Theses

Whole-body regeneration and asexual reproduction are widespread in the Metazoa, yet little is known about the genetic systems that drive the regeneration process outside of a few model organisms. While many animals utilize fission followed by regeneration to accomplish asexual reproduction, Convolutriloba longifissura is unusual in performing a double fission, requiring significant anterior-posterior and medial-lateral regeneration that alters the orthogonal body axes in adult tissues. To better understand the process of axial re-specification during postembryonic development, we studied the genetic mechanisms used by C. longifissura to modify and respecify axial polarity during asexual reproduction and subsequent regeneration. Because conserved signaling …

Implications Of Toll-Like Receptor 10 Mutants In Cell Signaling, Sonali Joshi

Undergraduate Honors Theses

Our innate immune system serves the important purpose of quickly recognizing microbial pathogens and providing the first line of defense against infection. Toll-Like Receptors (TLRs) are one class of receptors responsible for enacting our bodies’ innate immune responses. Specifically, TLR10 is an extremely under-researched receptor. Some naturally occurring mutations in TLR10 have been linked with a worsening prognosis of rheumatoid arthritis, with previous research uncovering a mutant form of TLR10 that impairs innate immune system-controlled inflammatory responses. However, other research has found that wild-type forms of TLR10 also contribute to pro-inflammatory pathways in healthy cells. To better understand the wild-type …

Disruption Of Play Behavior Influences Development Of Caution In Juvenile Ground Squirrels, William Ryan

Master's Theses

Play behavior is ubiquitous among young mammals and has a variety of important influences on early development. I evaluated possible influences of social play behavior on the development of temperament in juvenile Belding’s ground squirrels (Urocitellus beldingi), using the caution/boldness spectrum as a measure of temperament. I disrupted the play behavior of juvenile U. beldingi by walking toward juveniles at play until the play interactions stopped. I used undisrupted juveniles and juveniles whose non-play behavior was disrupted at regular intervals as controls. Caution was measured with behavioral tests during which a human intruder walked toward a squirrel and recorded the …

Investigation Of The Role Of Dax-1 In The Hpa Axis In Human Adrenal Cells, Katerina N. Fargas

Undergraduate Honors Theses

DAX-1 is a nuclear hormone orphan receptor that plays a key role in the development of reproductive tissues and steroid hormone production. The hypothalamic-pituitary-adrenal axis is the pathway for steroid hormone production, primarily glucocorticoids, mineralocorticoids, and adrenal androgens, in the human body. The mechanisms of DAX-1 in hormone production in the adrenal cortex of the HPA axis are not completely understood and, therefore, were the focus of this honors thesis research project. Due to the high level of DAX-1 expression, SW13 adrenal carcinoma cells were conducted for these experiments. We investigated whether glucocorticoids, specifically the synthetic glucocorticoid dexamethasone, had any …

Mechanisms Of Substrate Selectivity And Transport By Escherichia Coli Atp- Binding Cassette (Abc) Transporter Metni, Yuchun Chen

Master's Theses

ABC transporters are central in many cellular functions including nutrient uptake, signal transduction membrane assembly, and cellular detoxification. Both structural and functional studies have revealed insights into the high-affinity uptake mechanism of the MetNI methionine ABC transporter. Using the energy from ATP binding and hydrolysis, the MetNI-Q system can import L-Met, D-Met, and other methionine derivatives against concentration gradients. Many mechanistic studies of ABC transporter propose a model in which cognate binding proteins sequester substrates in the periplasm and deliver them to the transporter. In contrast, recent in vivo and crystallographic studies of MetNI-Q suggest that some substrates may be …

Climate-Related Declines In Reproductive Output In A High-Elevation Population Of Ground Squirrels, Kayleigh Little

Undergraduate Honors Theses

Beginning in the 1900s, changes in climate have resulted in an increase in global temperatures. This warming has driven behavioral and demographic changes within a multitude of species worldwide. Historically, small mammal populations have responded to changes in temperature by adjusting their geographic ranges. As temperatures rise, high-elevation populations may no longer be able to adjust as they have before. In this study, we evaluated possible effects of climate change on a high-elevation population of Belding’s ground squirrels (Urocitellus beldingi) at Tioga Pass in Northern California. We used local climate data and long-term population data from 1994 to …

Monitoring The Abundance Of Microplastics In Mussels And Marine Ecosystems May Indicate Human Health Risks., Puja Kaur Janda

Master's Projects and Capstones

The numerous scientific methodologies and procedures used in marine research on microplastic pollution restrict our present understanding of this severe environmental issue threatening marine organisms and human health. The abundance and bioaccumulation of microplastic in the food web is causing adverse implications for marine wildlife and potentially human health via seafood consumption. The absence of standardized methods for quantifying and characterizing microplastics is a major limiting factor in comparing current research. To evaluate the issue of monitoring microplastics in the marine environment, I conducted a Strengths, Weaknesses, Opportunities, and Threats (SWOT) analysis on mussels as a biomonitoring tool. Because mussels …

How Environmental Change Will Impact Mosquito-Borne Diseases, Arsal Khan

Master's Projects and Capstones

Mosquitos, the most lethal species throughout human history, are the most prevalent source of vector-borne diseases and therefore a major global health burden. Mosquito-borne disease incidence is expected to shift with environmental change. These changes can be predicted using species distribution models. With the wide variety of methods used for models, consensus for improving accuracy and comparability is needed. A comparative analysis of three recent modeling approaches revealed that integrating modeling techniques compensates for trade-offs associated with a singular approach. An area that represents a critical gap in our ability to predict mosquito behavior in response to changing climate factors, …

Assessing The Efficacy Of California’S Wildfire And Forest Resilience Action Plan, Chloe Nelson

Master's Projects and Capstones

California’s wildfire threat eclipses current forestry management and wildfire mitigation strategies in place to protect people, infrastructure, and the natural environment. Climate change escalates wildfire risks with declining water supply coupled with hotter, drier conditions. California’s Wildfire and Forest Resilience Action Plan attempts to integrate and build upon previously successful wildfire resilience plans to amplify the scale and pace of the state’s land management and community protections. This research assesses the plan’s efficacy to respond to the growing wildfire threat. This study investigates if there is equitable planning for the needs of high-wildfire risk groups living in the WUI and …

Impacts Of Sea Ice Loss On Polar Bear Diet, Prey Availability, Foraging Behaviors, And Human-Bear Interactions In The Arctic, Jasmin Chen

Master's Projects and Capstones

Anthropogenic-induced climate change has warmed the Arctic 2-3 times faster than the rest of the world, causing sea ice declines that introduce challenges for specialist species, such as polar bears, in adapting to rapid environmental changes. Comparative and quantitative analyses of three Hudson Bay polar bear subpopulations were used to determine the impacts of sea ice loss on polar bear diet, prey availability, foraging behaviors, and human-bear interactions in the Arctic. The study reveal that Hudson Bay polar bears experience the most severe impacts from sea ice declines, resulting in a 30% population decline. Due to their smaller body size, …

Impacts Of Nitrogen Enrichment On Corals – A Remote Sensing Approach, David E. Carrasco

Master's Projects and Capstones

Anthropogenic activities have resulted in ever-increasing threats to coral reefs globally, wherein the rate of environmental changes have exceeded the historical capacity of corals to adapt. This has threatened the persistence of coral ecosystems and their associated ecosystem services, which billions of people rely on for their livelihoods. The most prevalent stressor is nitrogen enrichment, which while present naturally, is exacerbated by the anthropogenic input of nutrients via the discharge of agricultural and urban waste waters. The focus is to answer the central research question of how nitrogen enrichment impacts corals, and how it interacts with other stressors with particular …

A Circular Economy Approach To Improve E-Waste Recycling In California: Economic Potential And Policy Options, Kripa Shah

Master's Projects and Capstones

The higher consumption rates of electronic devices along with their short life cycles and few repair options poses a huge challenge for E-waste industries to manage them effectively. E-waste comprises of hazardous materials and toxic constituents that can affect the environment and public health through improper disposal. However, they also contain few valuable materials that, if recovered, can reduce the dependence on virgin raw materials. Circular economy has the potential to utilize these valuable materials and gain environment and socio-economic benefits. The research explains how the adoption of a circular economy approach can help improve the E-waste recycling in California …

Joint Management Of Upland & Aquatic Habitat For The California Red-Legged Frog & California Tiger Salamander, Kyle E. Verblaauw

Master's Projects and Capstones

As federally and state protected amphibians, the California tiger salamander (Ambystoma californiense) and the California red-legged frog (Rana draytonii) are recipients of ample management focus. Both species face a variety of threats, including habitat loss and alteration, introduction of non-native species, spread of disease, and effects of climate change. While management plans for the California tiger salamander and California red-legged frog exist, they frequently do not consider both species in tandem and often contain multiple shortcomings. This document aims to address the shortfalls of current management by providing practical recommendations for jointly managing the upland and …

Coyote Management In San Francisco, Matthew Greer

Master's Projects and Capstones

As cities in North America grow, coyotes have become progressively more important species in urban ecosystems. Moreover, as coyotes increasingly use these urban spaces, human-coyote conflicts have become more common, creating a need for new management strategies. This paper will explore how the city of San Francisco could create a new coyote management plan for its highly visible coyote population. San Francisco has had a persistent coyote population since the early 2000s (Todd, 2018). Currently, the coyote population is close to 100 individuals and is still growing (J. Young, Presidio Trust, pers. comm.). To explore this topic a literature review …

Examination Of Methylation Status And Occupancy Of Dna Methylation Modifying Proteins On Regulatory Regions Of The Dax-1 Gene, Caroline P. Riedstra

Master's Theses

Epigenetic modifications influence gene expression and thereby play a pivotal role in development and disease. Misregulation and mutations in the DAX-1 gene, or Dosage-Sensitive Sex Reversal, Adrenal Hypoplasia Congenita, Critical Region on the X chromosome, gene 1, have been implicated in Adrenal Hypoplasia Congenita (AHC) and Dosage Sensitive Sex Reversal (DSS). The orphan nuclear hormone receptor DAX-1 is expressed predominantly in tissues such as the testes, ovaries, breast, adrenal cortex, and lung. Critically, DAX-1 may serve as an indicator of aberrant growth in these tissues. Here we hypothesize that DAX-1 is epigenetically regulated, specifically in cancer cells, thereby reducing its …

Investigating The Mechanism Of The Escherichia Coli Atp-Binding Cassette (Abc) Transporter Metni, Matthew Foronda

Master's Theses

Chemical homeostasis is a baseline requirement for any cell to survive. ATP-binding cassette (ABC) transporters play a vital role in homeostasis by importing nutrients and exporting toxins against their concentration gradients by utilizing the energy of ATP hydrolysis. Malfunctioning ABC transporters cause a variety of health problems, including cystic fibrosis, Stargardt’s disease (vision loss), and the development of drug-resistant tumors. An important step in solving these medical issues is to first understand the structure and mechanism of ABC transporters. Various studies have made great strides in depicting the structure and details of different ABC transporters and their mechanisms, however, many …