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Full-Text Articles in Life Sciences

Development Of A Highly Responsive Leaf-Based Regeneration System For Peperomia Species, Mohammad Ahmadabadi, Ralph Bock Jan 2010

Development Of A Highly Responsive Leaf-Based Regeneration System For Peperomia Species, Mohammad Ahmadabadi, Ralph Bock

Turkish Journal of Botany

Several species in the genus Peperomia Ruiz & Pav. have giant chloroplasts in the palisade parenchyma of their leaves. Because of this unique feature, Peperomia metallica L. Linden & Rodigas has become a valuable model plant for studying plastid biology. However, the use of Peperomia for molecular studies has been limited by the lack of efficient regeneration and transformation protocols. In this work, we aimed to develop a reliable regeneration system for Peperomia from leaf explants. Using MS medium complemented with different combinations of phytohormones, a highly efficient in vitro regeneration protocol was developed for P. metallica and P. peduncularis, …


Indole-3-Acetic Acid And Gibberellic Acid Production In Aspergillus Niger, Işil Seyi̇s Bi̇lkay, Şafak Karakoç, Ni̇lüfer Aksöz Jan 2010

Indole-3-Acetic Acid And Gibberellic Acid Production In Aspergillus Niger, Işil Seyi̇s Bi̇lkay, Şafak Karakoç, Ni̇lüfer Aksöz

Turkish Journal of Biology

The effects of incubation time, temperature, pH, and agitation on indole-3- acetic acid and gibberellic acid production in Aspergillus niger were studied. For indole-3-acetic acid production, 6 days of incubation at 25 °C and pH 6.0 was found to be optimum. Optimum conditions for gibberellic acid production were 12 days of incubation at 30 °C and pH 5.0. Agitation increased both indole-3-acetic acid and gibberellic acid production.


The Effects Of Gibberellic Acid And Kinetin On Overcoming The Effects Of Juglone Stress On Seed Germination And Seedling Growth, İrfan Terzi̇, İsmai̇l Kocaçalişkan Jan 2010

The Effects Of Gibberellic Acid And Kinetin On Overcoming The Effects Of Juglone Stress On Seed Germination And Seedling Growth, İrfan Terzi̇, İsmai̇l Kocaçalişkan

Turkish Journal of Botany

Juglone (5-hydroxy-1,4-naphthoquinone) is an allelochemical responsible for walnut allelopathy. The effects of gibberellic acid (GA_{3}) and kinetin (KIN) on overcoming the effects of juglone stress on seed germination and seedling growth were investigated in barley, wheat, cucumber, alfalfa, and tomato. Seeds pre-treated with plant growth regulators were used to test their effects on the alleviation of juglone stress. It was observed that seed germination in tomato and wheat was inhibited by juglone and that the plant growth regulators alleviated it significantly. Elongation and dry weight of the seedlings of all the species used in the study were reduced significantly by …


Effects Of Moist Chilling, Gibberellic Acid, And Scarification On Seed Dormancy In The Rare Endemic Pedicularis Olympica (Scrophulariaceae), Serap Kirmizi, Gürcan Güleryüz, Hülya Arslan, Fatma Selcen Sakar Jan 2010

Effects Of Moist Chilling, Gibberellic Acid, And Scarification On Seed Dormancy In The Rare Endemic Pedicularis Olympica (Scrophulariaceae), Serap Kirmizi, Gürcan Güleryüz, Hülya Arslan, Fatma Selcen Sakar

Turkish Journal of Botany

We investigated the germination requirements of a rare endemic plant species, Pedicularis olympica Boiss. (Scrophulariaceae), which grows in wet habitats on Uludağ Mountain, Turkey. We studied the effects of scarification, moist chilling (+4 °C) for 15 days, different doses of gibberellic acid (GA_{3}; 100, 150, and 250 ppm) and combined hormone and moist chilling treatments under dark (20 °C) and photoperiod (20/10 °C; 12/12 h, respectively) conditions. GA_{3} was able to break dormancy. The highest germination rate was found in the seeds treated with 250 ppm GA_{3}; 64% of these seeds germinated when treated with moist chilling and incubated in …