Open Access. Powered by Scholars. Published by Universities.®
Articles 1 - 8 of 8
Full-Text Articles in Life Sciences
Identification Of Conserved Mirna Molecules In Einkorn Wheat (Triticum Monococcum Subsp. Monococcum) By Using Small Rna Sequencing Analysis, Ercan Selçuk Ünlü, Sara Bataw, Di̇dem Şen, Yunus Şahi̇n, Nusret Zenci̇rci̇
Identification Of Conserved Mirna Molecules In Einkorn Wheat (Triticum Monococcum Subsp. Monococcum) By Using Small Rna Sequencing Analysis, Ercan Selçuk Ünlü, Sara Bataw, Di̇dem Şen, Yunus Şahi̇n, Nusret Zenci̇rci̇
Turkish Journal of Biology
Triticum monococcum subsp. monococcum as a first cultivated diploid wheat species possesses desirable agronomic and quality characteristics. Drought and salinity are the most dramatic environmental stress factors that have serious impact on yield and quality of crops; however, plants can use alternative defense mechanisms against these stresses. The posttranscriptional alteration of gene expression by microRNAs (miRNAs) is one of the most conserved mechanisms. In plant species including wheat genomes, miRNAs have been implicated in the management of salt and drought stress; however, studies on einkorn wheat (Triticum monococcum subsp. monococcum) are not yet available. In this study, we aimed to …
Effect Of Polyamines On Somatic Embryogenesis Via Mature Embryo In Wheat, Murat Aydin, Arash Hossein Pour, Kami̇l Hali̇loğlu, Meti̇n Tosun
Effect Of Polyamines On Somatic Embryogenesis Via Mature Embryo In Wheat, Murat Aydin, Arash Hossein Pour, Kami̇l Hali̇loğlu, Meti̇n Tosun
Turkish Journal of Biology
Plant tissue culture via somatic embryogenesis plays a key role in wheat genetic transformation studies. Therefore, the production of embryogenic calli with a high regeneration capacity is a prerequisite for efficient plant regeneration. Although immature embryos are the best type of explants for plant regeneration via somatic embryogenesis, the use of mature embryos has remarkable advantages compared to immature embryos as explants. However, the regeneration capacity of mature embryos is still lower than that of immature embryos. Plant regeneration from somatic embryos can only occur when they are mature enough. Moreover, the maturation of somatic embryos and their conversion into …
Enhanced Salt Tolerance Of Transgenic Tobacco Expressing A Wheat Salt Tolerance Gene, Musa Kavas, Mehmet Cengi̇z Baloğlu, Ayşe Meral Yücel, Hüseyi̇n Avni̇ Öktem
Enhanced Salt Tolerance Of Transgenic Tobacco Expressing A Wheat Salt Tolerance Gene, Musa Kavas, Mehmet Cengi̇z Baloğlu, Ayşe Meral Yücel, Hüseyi̇n Avni̇ Öktem
Turkish Journal of Biology
Soil salinity is one of the most important limiting factors of agricultural productivity in the world. The Triticum aestivum salt tolerance-related gene (TaSTRG) possesses a functional response to salt and drought stress conditions. A variety of stress factors, such as salt, drought, abscisic acid, and cold, may induce the expression of TaSTRG in wheat. In this study, the TaSTRG gene was transferred to tobacco via Agrobacterium-mediated transformation. Overexpression of TaSTRG in transgenic tobacco plants indicated higher salt tolerance and mediated more vigorous growth than in wild-type plants. Under salt stress conditions, the transgenic tobacco plants had higher germination and survival …
Differential Expression Analysis Of Defense-Related Genes Responsive To Tilletia Indica Infection In Wheat, Aradhika Tripathi, Rashmi Aggarwal, Anita Yadav
Differential Expression Analysis Of Defense-Related Genes Responsive To Tilletia Indica Infection In Wheat, Aradhika Tripathi, Rashmi Aggarwal, Anita Yadav
Turkish Journal of Biology
Karnal bunt caused by Tilletia indica is a quarantine disease responsible for qualitative and quantitative losses in wheat. The present study aimed to determine the expression of defense-related genes involved in early stages of infection in response to T. indica by using the differential display reverse transcriptase-PCR method (DDRT-PCR) in susceptible (WL 711) and resistant (HD 29) cultivars of wheat. The DDRT-PCR banding profiles generated by the random primers in combination with oligo-dT primers of resistant and susceptible wheat plants were evaluated at 0 h, 12 h, 24 h, and 48 h after inoculation. Out of 9 sets of primers, …
Germination Characteristics And Molecular Characterizations Of Some Wheat Varieties In Sulaimanyah By Ssr Marker, Nawroz Abdul-Razzak Tahir
Germination Characteristics And Molecular Characterizations Of Some Wheat Varieties In Sulaimanyah By Ssr Marker, Nawroz Abdul-Razzak Tahir
Turkish Journal of Biology
The genetic diversity of 11 bread and durum wheat varieties was estimated using germination characteristic and molecular analysis. The phenotypic data showed highly significant differences between cultivars for germination speed, germination percentage, seedling length, fresh weight, and dry weight. Simple sequence repeats (SSRs) were used to examine the genomic diversity of wheat (Triticum aestivum L. and Triticum durum L.). Eleven wheat varieties of diverse origins were analyzed with 12 selected SSRs to provide uniform and maximum genome coverage. A total of 18 bread wheat and 20 durum wheat polymorphic allelic variants were detected, ranging from 1 to 4 per locus …
Plant Growth Promoting Properties Of Rhizobacteria Isolated From Wheat And Pea Grown In Loamy Sand Soil, Dilfuza Egamberdieva
Plant Growth Promoting Properties Of Rhizobacteria Isolated From Wheat And Pea Grown In Loamy Sand Soil, Dilfuza Egamberdieva
Turkish Journal of Biology
Microbes are important catalysts to regulate functional properties of terrestrial ecosystems. In this study, rhizosphere and phyllosphere bacteria were isolated from wheat and peas and examined for their plant growth promoting properties. The effects of bacterial inoculants on the growth of peas and wheat were studied in a series of pot experiments using loamy sand soil. The results showed that the colonisation of bacteria was higher in the rhizosphere as compared to the phyllosphere of both plants. Bacterial strains were identified as Pseudomonas, Bacillus, Kocuria, and Microbacterium, and Cellulomonas species. The response of wheat and peas when inoculated with bacteria …
Genetic Identification Of Diploid And Tetraploid Wheat Species With Rapd Markers, Rami̇z Tagi̇ Ali̇yev, Mehraj Ali̇ Abbasov, Alamdar Charkaz Mammadov
Genetic Identification Of Diploid And Tetraploid Wheat Species With Rapd Markers, Rami̇z Tagi̇ Ali̇yev, Mehraj Ali̇ Abbasov, Alamdar Charkaz Mammadov
Turkish Journal of Biology
The genetic diversity of 10 diploid and tetraploid wheat species was estimated using random amplified polymorphic DNA (RAPD) markers. Two species from diploid [Triticum boeticum (wild), Triticum monococcum] and five from tetraploid wheats [Triticum dicoccoides var. arabicum (wild), Triticum dicoccum var. farrum, Triticum dicoccum var. atratum, Triticum durum var. hordeiforme (Bereketli 95), Triticum durum var. leucurum (Sharq), Triticum turgidum var. alboyadurum, Triticum turgidum var. salomonis and Triticum persicum] were included for the analyses. Jacard's cluster analysis algorithm was used to determine genetic similarities. There were two main classes in dendrogram: the varieties [T. boeticum, T. dicoccoides var. arabicum, T. dicoccum …
Efficient Cloning Of The Rare Wheat (Triticum Aestivum L.) Transcripts, Muharrem Di̇lbi̇rli̇ği̇, Mustafa Erayman
Efficient Cloning Of The Rare Wheat (Triticum Aestivum L.) Transcripts, Muharrem Di̇lbi̇rli̇ği̇, Mustafa Erayman
Turkish Journal of Biology
Expressed sequence tag (EST) technology has spurred targeting the functional portion of genomes for several organisms. Constructed redundant complementary DNA (cDNA) libraries have targeted a majority of constitutive and over-expressed genes. However, the majority of lowly expressed and un-induced genes have not been able to be easily tagged and cloned. The objective of this study was to clone low and rarely expressed transcripts in wheat. Using a combination of a polyacrylamide gel and a motif-based PCR amplification, we cloned 144 bands containing 189 wheat fragments. Sequence analysis revealed 74 contigs, of which only 22 being present in database. The remaining …