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Varying Weed Densities Alter The Corn Transcriptome, Highlighting A Core Set Of Weed-Induced Genes And Processes With Potential For Manipulating Weed Tolerance, David P. Horvath, Sharon A. Clay, Stephanie A. Bruggeman, James V. Anderson, Wun S. Chao, Kathleen Yeater Nov 2019

Varying Weed Densities Alter The Corn Transcriptome, Highlighting A Core Set Of Weed-Induced Genes And Processes With Potential For Manipulating Weed Tolerance, David P. Horvath, Sharon A. Clay, Stephanie A. Bruggeman, James V. Anderson, Wun S. Chao, Kathleen Yeater

Agronomy, Horticulture and Plant Science Faculty Publications

The phenological responses of corn (Zea mays L.) to competition with increasing densities of winter canola (Brassica napus L.) as the weedy competitor were investigated. Changes in the corn transcriptome resulting from varying weed densities were used to identify genes and processes responsive to competition under controlled conditions where light, nutrients, and water were not limited. Increasing densities of weeds resulted in decreased corn growth and development and increased the number and expression intensity of competition-responsive genes. The physiological processes identified in corn that were consistently induced by competition with weeds included protein synthesis and various transport functions. …


Genetic Analysis Of Stilbenoid Profiles In Grapevine Stems Reveals A Major Mqtl Hotspot On Chromosome 18 Associated With Disease-Resistance Motifs, Soon L. Teh, Bety Rostandy, Mani Awale, James J. Luby, Anne Fennell, Adrian D. Hegeman Nov 2019

Genetic Analysis Of Stilbenoid Profiles In Grapevine Stems Reveals A Major Mqtl Hotspot On Chromosome 18 Associated With Disease-Resistance Motifs, Soon L. Teh, Bety Rostandy, Mani Awale, James J. Luby, Anne Fennell, Adrian D. Hegeman

Agronomy, Horticulture and Plant Science Faculty Publications

Grapevine (Vitis spp.) contains a wealth of phytochemicals that have received considerable attention due to health-promoting properties and biological activities as phytoalexins. To date, the genetic basis of the quantitative variations for these potentially beneficial compounds has been limited. Here, metabolic quantitative trait locus (mQTL) mapping was conducted using grapevine stems of a segregating F2 population. Metabolic profiling of grapevine stems was performed using liquid chromatography–high-resolution mass spectrometry (LC-HRMS), resulting in the detection of 1317 ions/features. In total, 19 of these features matched with literature-reported stilbenoid masses and were genetically mapped using a 1449-SNP linkage map and R/qtl …


Ethanol Sprays To Release Grapevine Bud Dormancy: A Potential Alternative To Cyanamides, Christian Chervin, Anne Fennell Oct 2019

Ethanol Sprays To Release Grapevine Bud Dormancy: A Potential Alternative To Cyanamides, Christian Chervin, Anne Fennell

Agronomy, Horticulture and Plant Science Faculty Publications

Aim: Grape growers sometimes use cyanamides (calcium or hydrogen) to release bud dormancy in warm climate regions, where the chilling requirement has not been met during winter. However, these products can cause damage to plants and are dangerous to handle, so alternatives would be welcomed by growers. Connections between metabolisms of ethanol, ethylene and cyanide revealed by previous studies led us to test the potential of ethanol sprays on bud break and early shoot growth.
Methods and results: Trials were performed over three years on Vitis vinifera grapevines trained in Guyot or cordon, and on cuttings in growth chambers. Cultivars …


United States Midwest Soil And Weather Conditions Influence Anaerobic Potentially Mineralizable Nitrogen, Jason D. Clark, Kristen S. Veum, Fabian G. Fernandez, James J. Camberato, Paul R. Carter, Richard B. Ferguson, David W. Franzen, Daniel E. Kaiser, Newell R. Kitchen, Carrie A.M. Laboski, Emerson D. Nafziger, John E. Sawyer, John F. Shanahan Sep 2019

United States Midwest Soil And Weather Conditions Influence Anaerobic Potentially Mineralizable Nitrogen, Jason D. Clark, Kristen S. Veum, Fabian G. Fernandez, James J. Camberato, Paul R. Carter, Richard B. Ferguson, David W. Franzen, Daniel E. Kaiser, Newell R. Kitchen, Carrie A.M. Laboski, Emerson D. Nafziger, John E. Sawyer, John F. Shanahan

Agronomy, Horticulture and Plant Science Faculty Publications

Nitrogen provided to crops through mineralization is an important factor in N management guidelines. Understanding of the interactive effects of soil and weather conditions on N mineralization needs to be improved. Relationships between anaerobic potentially mineralizable N (PMNan) and soil and weather conditions were evaluated under the contrasting climates of eight US Midwestern states. Soil was sampled (0–30 cm) for PMNan analysis before pre-plant N application (PP0N) and at the V5 development stage from the pre-plant 0 (V50N) and 180 kg N ha−1 (V5180N) rates and incubated for 7, 14, …


Management Considerations For Palmer Amaranth In A Northern Great Plains Soybean Production System, Brian Vab De Stroet, Sharon Clay Aug 2019

Management Considerations For Palmer Amaranth In A Northern Great Plains Soybean Production System, Brian Vab De Stroet, Sharon Clay

Agronomy, Horticulture and Plant Science Faculty Publications

Palmer amaranth (Amaranthus palmeri S. Watson) was first observed in a South Dakota field in 2015. This study assessed Palmer amaranth growth based on planting date (PD), impact on soybean [Glycine max (L.) Merr.] yield, and response of seedlings of South Dakota biotype seedlings to herbicides with different mechanisms of action (MOA). Soybean yield loss was influenced by Palmer amaranth density in 2016 (p = 0.001), with yield losses of 33% at densities greater than 15 plants m−2 (R2 = 0.65), although yield losses at low densities were greater than predicted by the fitted …


Predicting Economic Optimal Nitrogen Rate With The Anaerobic Potentially Mineralizable Nitrogen Test, Jason D. Clark, Fabian G. Fernandez, Kristen S. Veum, James J. Camberato, Paul R. Carter, Richard B. Ferguson, David W. Franzen, Daniel E. Kaiser, Newell R. Kitchen, Carrie A.M. Laboski, Emerson D. Nafziger, Carl J. Rosen, John E. Sawyer, John F. Shanahan Aug 2019

Predicting Economic Optimal Nitrogen Rate With The Anaerobic Potentially Mineralizable Nitrogen Test, Jason D. Clark, Fabian G. Fernandez, Kristen S. Veum, James J. Camberato, Paul R. Carter, Richard B. Ferguson, David W. Franzen, Daniel E. Kaiser, Newell R. Kitchen, Carrie A.M. Laboski, Emerson D. Nafziger, Carl J. Rosen, John E. Sawyer, John F. Shanahan

Agronomy, Horticulture and Plant Science Faculty Publications

Estimates of mineralizable N with the anaerobic potentially mineralizable N (PMNan) test could improve predictions of corn (Zea mays L.) economic optimal N rate (EONR). A study across eight US midwestern states was conducted to quantify the predictability of EONR for single and split N applications by PMNan. Treatment factors included different soil sample timings (pre-plant and V5 development stage), planting N rates (0 and 180 kg N ha−1), and incubation lengths (7, 14, and 28 d) with and without initial soil NH4–N included with PMNan. Soil was sampled …


Qtl × Environment Interactions Underlie Adaptive Divergence In Switchgrass Across A Large Latitudinal Gradient, David B. Lowry, John T. Lovell, Li Zhang, Jason Bonnette, Philip A. Fay, Robert B. Mitchell, John Lloyd-Reilley, Arvid R. Boe, Et. Al Jun 2019

Qtl × Environment Interactions Underlie Adaptive Divergence In Switchgrass Across A Large Latitudinal Gradient, David B. Lowry, John T. Lovell, Li Zhang, Jason Bonnette, Philip A. Fay, Robert B. Mitchell, John Lloyd-Reilley, Arvid R. Boe, Et. Al

Agronomy, Horticulture and Plant Science Faculty Publications

Local adaptation is the process by which natural selection drives adaptive phenotypic divergence across environmental gradients. Theory suggests that local adaptation results from genetic trade-offs at individual genetic loci, where adaptation to one set of environmental conditions results in a cost to fitness in alternative environments. However, the degree to which there are costs associated with local adaptation is poorly understood because most of these experiments rely on two-site reciprocal transplant experiments. Here, we quantify the benefits and costs of locally adaptive loci across 17° of latitude in a four-grandparent outbred mapping population in outcrossing switchgrass (Panicum virgatum L.), …


Chemical Amendments Of Dryland Saline–Sodic Soils Did Not Enhance Productivity And Soil Health In Fields Without Effective Drainage, Girma A. Birru, David E. Clay, Thomas M. Desutter, Cheryl L. Reese, Ann C. Kennedy, Sharon A. Clay, Stephanie A. Bruggeman, Rachel K. Owen, Douglas D. Malo Apr 2019

Chemical Amendments Of Dryland Saline–Sodic Soils Did Not Enhance Productivity And Soil Health In Fields Without Effective Drainage, Girma A. Birru, David E. Clay, Thomas M. Desutter, Cheryl L. Reese, Ann C. Kennedy, Sharon A. Clay, Stephanie A. Bruggeman, Rachel K. Owen, Douglas D. Malo

Agronomy, Horticulture and Plant Science Faculty Publications

A common restoration treatment for saline–sodic soils involves improving soil drainage, applying soil amendments (e.g., CaSO4, CaCl2, or elemental S), and leaching with water that has a relatively low electrical conductivity. However, due to high subsoil bulk densities and low drainable porosities, these treatments many not be effective in glaciated dryland systems. A 3-yr field study conducted in three model systems determined the impact of chemical amendments (none, CaCl2, CaSO4, and elemental S) on plant growth, microbial composition, temporal changes in electrical conductivity (ECe ), and the relative sodium content (%Na). …


Phomopsis Stem Canker Of Sunflower In North America: Correlation With Climate And Solutions Through Breeding And Management, Brent S. Hulke, Samuel G. Markell, Nolan C. Kane, Febina M. Mathew Mar 2019

Phomopsis Stem Canker Of Sunflower In North America: Correlation With Climate And Solutions Through Breeding And Management, Brent S. Hulke, Samuel G. Markell, Nolan C. Kane, Febina M. Mathew

Agronomy, Horticulture and Plant Science Faculty Publications

Climate change is occurring in the central US and is interacting with agroecological factors to increase biotic stress in sunflower. Certain species of Diaporthe cause Phomopsis stem canker in sunflower and other dicotyledonous weeds and crops. The increase in precipitation already observed in the states of North Dakota, South Dakota, and Minnesota have increased the chances of outbreaks of necrotrophic pathogens, like Diaporthe. We discuss how climate trends, combined with technological, management, and economic interactions, are correlated with increasing incidence of Phomopsis stem canker in these and adjacent areas in North America. Further, we discuss management options and the …


Nitrogen Requirements Of Ethiopian Mustard For Biofuel Feedstock In South Dakota, Phillip Alberti, Shannon Osborne, Febina Mathew, Shakaut Ali, Heidi Sieverding, Sandeep Kumar, Thandiwe Nieya Mar 2019

Nitrogen Requirements Of Ethiopian Mustard For Biofuel Feedstock In South Dakota, Phillip Alberti, Shannon Osborne, Febina Mathew, Shakaut Ali, Heidi Sieverding, Sandeep Kumar, Thandiwe Nieya

Agronomy, Horticulture and Plant Science Faculty Publications

Ethiopian mustard (Brassica carinata A. Braun) is a non-food oilseed crop that has received attention for its potential as a low-input biofuel feedstock suitable for production in the semiarid regions of the Northern Great Plains (NGP). Because B. carinata is a new crop to the NGP, the best management practices have yet to be developed. The objective of the study was to evaluate the effects of N fertilizer rate on seed yield, seed oil concentration, and oil yield of B. carinata and to determine the economic optimum N fertilizer rates. Field studies were conducted at two locations in South …


Soil And Land- Use Change Sustainability In The Northern Great Plains Of The Usa, Deepak Raj Joshi, David E. Clay, Alexander Smart, Sharon A. Clay, Tulsi P. Kharel, Umakanta Mishra Mar 2019

Soil And Land- Use Change Sustainability In The Northern Great Plains Of The Usa, Deepak Raj Joshi, David E. Clay, Alexander Smart, Sharon A. Clay, Tulsi P. Kharel, Umakanta Mishra

Agronomy, Horticulture and Plant Science Faculty Publications

In the Northern Great Plains (NGP), the combined impacts of land-use and climate variability have the potential to place many soils on the tipping point of sustainability. The objectives of this study were to assess if the conversion of grassland to croplands occurred on fragile landscapes in the North America Northern Great Plains. South Dakota and Nebraska were selected for this study because they are located in a climate transition zone. We visually classified 43,200 and 38,400 points in South Dakota and Nebraska, respectively, from high-resolution imagery in 2006, 2012, and 2014 into five different categories (cropland, grassland, habitat, NonAg, …


Transcriptomic Response Is More Sensitive To Water Deficit In Shoots Than Roots Of Vitis Riparia (Michx.), Vedbar Singh Khada, Kimberly Vaughn, Juan Xie, Padmapriya Swaminathan, Qin Ma, Grant Cramer, Anne Y. Fennell Dr Feb 2019

Transcriptomic Response Is More Sensitive To Water Deficit In Shoots Than Roots Of Vitis Riparia (Michx.), Vedbar Singh Khada, Kimberly Vaughn, Juan Xie, Padmapriya Swaminathan, Qin Ma, Grant Cramer, Anne Y. Fennell Dr

Agronomy, Horticulture and Plant Science Faculty Publications

Background: Drought is an important constraint on grapevine sustainability. Vitis riparia, widely used in rootstock and scion breeding, has been studied in isolated leaf drying response studies; however, it is essential to identify key root and shoot water deficit signaling traits in intact plants. This information will aid improved scion and rootstock selection and management practices in grapevine. RNAseq data were generated from V. riparia roots and shoots under water deficit and well-watered conditions to determine root signaling and shoot responses to water deficit.
Results: Shoot elongation, photosynthetic rate, and stomatal conductance were significantly reduced in water deficit (WD) treated …


Iris-Eda: An Integrated Rna-Seq Interpretation System For Gene Expression Data Analysis, Brandon Monier, Adam Mcdermaid, Cankun Wang, Jing Zhao, Allison Miller, Anne Fennell, Qin Ma Feb 2019

Iris-Eda: An Integrated Rna-Seq Interpretation System For Gene Expression Data Analysis, Brandon Monier, Adam Mcdermaid, Cankun Wang, Jing Zhao, Allison Miller, Anne Fennell, Qin Ma

Agronomy, Horticulture and Plant Science Faculty Publications

Next-Generation Sequencing has made available substantial amounts of large-scale Omics data, providing unprecedented opportunities to understand complex biological systems. Specifically, the value of RNA-Sequencing (RNA-Seq) data has been confirmed in inferring how gene regulatory systems will respond under various conditions (bulk data) or cell types (single-cell data). RNA-Seq can generate genome-scale gene expression profiles that can be further analyzed using correlation analysis, co-expression analysis, clustering, differential gene expression (DGE), among many other studies. While these analyses can provide invaluable information related to gene expression, integration and interpretation of the results can prove challenging. Here we present a tool called IRIS-EDA, …


Evaluation Of Volatile Metabolites Emitted In-Vivo From Cold-Hardy Grapes During Ripening Using Spme And Gc-Ms: A Proof-Of-Concept, Somchai Rice, Devin L. Maurer, Anne Fennell, Murlidhar Dharmadhikari, Jacek A. Koziel Feb 2019

Evaluation Of Volatile Metabolites Emitted In-Vivo From Cold-Hardy Grapes During Ripening Using Spme And Gc-Ms: A Proof-Of-Concept, Somchai Rice, Devin L. Maurer, Anne Fennell, Murlidhar Dharmadhikari, Jacek A. Koziel

Agronomy, Horticulture and Plant Science Faculty Publications

In this research, we propose a novel concept for a non-destructive evaluation of volatiles emitted from ripening grapes using solid-phase microextraction (SPME). This concept is novel to both the traditional vinifera grapes and the cold-hardy cultivars. Our sample models are cold-hardy varieties in the upper Midwest for which many of the basic multiyear grape flavor and wine style data is needed. Non-destructive sampling included a use of polyvinyl fluoride (PVF) chambers temporarily enclosing and concentrating volatiles emitted by a whole cluster of grapes on a vine and a modified 2 mL glass vial for a vacuum-assisted sampling of volatiles from …


Crop Residue Management Challenges: A Special Issue Overview, David Clay, Ronald Alverson, Jane M.F. Johnson, Douglas L. Karlen, Sharon Clay, Michael Q. Wang, Stephanie A. Bruggeman, Shaina Westhoff Jan 2019

Crop Residue Management Challenges: A Special Issue Overview, David Clay, Ronald Alverson, Jane M.F. Johnson, Douglas L. Karlen, Sharon Clay, Michael Q. Wang, Stephanie A. Bruggeman, Shaina Westhoff

Agronomy, Horticulture and Plant Science Faculty Publications

The amount of crop residues that can be sustainability removed is highly variable and is a function of many factors including the soil, climatic, and plant characteristics. For example, leaving an insufficient amount of crop residue on the soil surface can be detrimental for soil quality, result in loss of soil organic matter (SOM), and increase soil erosion, whereas leaving excessive amounts can impair soil-seed contact, immobilize N, and/or keep soils cool and wet. This special issue evolved as an outcome of, “Crop Residues for Advanced Biofuels: Effects on Soil Carbon” workshop held in Sacramento, CA, in 2017. The goal …


Effects Of Harvest Time On The Aroma Of White Wines Made From Cold-Hardy Brianna And Frontenac Gris Grapes Using Headspace Solid-Phase Microextraction And Gas Chromatography-Mass Spectrometry-Olfactometry, Somchai Rice, Madina Tursumbayeva, Matthew Clark, David Greenlee, Murlidhar Dharmadhikari, Anne Fennell Dr, Jacek A. Koziel Jan 2019

Effects Of Harvest Time On The Aroma Of White Wines Made From Cold-Hardy Brianna And Frontenac Gris Grapes Using Headspace Solid-Phase Microextraction And Gas Chromatography-Mass Spectrometry-Olfactometry, Somchai Rice, Madina Tursumbayeva, Matthew Clark, David Greenlee, Murlidhar Dharmadhikari, Anne Fennell Dr, Jacek A. Koziel

Agronomy, Horticulture and Plant Science Faculty Publications

The Midwest wine industry has shown a marked increase in growers, hectares planted, wineries, and wine production. This growth coincides with the release of cold-hardy cultivars such as Brianna and Frontenac gris, in 2001 and 2003, respectively. These white grape varieties account for one-third of the total area grown in the state of Iowa. It is generally accepted that the wine aroma profile plays a crucial role in developing a local, sustainable brand. However, the identity of Brianna/Frontenac Gris-based wine aromas and their link to the grape berry chemistry at harvest is unknown. This study aims to preliminarily characterize key …


Biogenic Volatiles Emitted From Four Cold-Hardy Grape Cultivars During Ripening, Somchai Rice, Devin L. Mauer, Anne Fennell, Murlidhar Dharmadhikari, Jacek A. Koziel Jan 2019

Biogenic Volatiles Emitted From Four Cold-Hardy Grape Cultivars During Ripening, Somchai Rice, Devin L. Mauer, Anne Fennell, Murlidhar Dharmadhikari, Jacek A. Koziel

Agronomy, Horticulture and Plant Science Faculty Publications

In this research dataset, we summarize for the first time volatile organic compounds (VOCs) emitted in vivo from ripening wine grapes. We studied four cold-hardy cultivars grown in the Midwestern U.S.: St. Croix, Frontenac, Marquette, and La Crescent. These cultivars have gained popularity among local growers and winemakers, but still very little is known about their performance compared with long-established V. vinifera grapes. Volatiles were collected using two novel approaches: biogenic emissions from grape clusters on a vine and single grape berries. A third approach was headspace collection of volatiles from crushed grapes. Solid-phase microextraction (SPME) was used to collect …


Identification And Characterization Of Mitogen-Activated Protein Kinase (Mapk) Genes In Sunflower (Helianthus Annuus L.), Surendra Neupane, Sarah E. Schweitzer, Achal Neupane, Ethan J. Anderson, Anne Fennell, Ruanbao Zhou, Madhav P. Nepal Jan 2019

Identification And Characterization Of Mitogen-Activated Protein Kinase (Mapk) Genes In Sunflower (Helianthus Annuus L.), Surendra Neupane, Sarah E. Schweitzer, Achal Neupane, Ethan J. Anderson, Anne Fennell, Ruanbao Zhou, Madhav P. Nepal

Agronomy, Horticulture and Plant Science Faculty Publications

Mitogen-Activated Protein Kinase (MAPK) genes encode proteins that regulate biotic and abiotic stresses in plants through signaling cascades comprised of three major subfamilies: MAP Kinase (MPK), MAPK Kinase (MKK), and MAPKK Kinase (MKKK). The main objectives of this research were to conduct genome-wide identification of MAPK genes in Helianthus annuus and examine functional divergence of these genes in relation to those in nine other plant species (Amborella trichopoda, Aquilegia coerulea, Arabidopsis thaliana, Daucus carota, Glycine max, Oryza sativa, Solanum lycopersicum, Sphagnum fallax, and Vitis vinifera), representing diverse taxonomic groups of …


Soil Quality And Region Influence Performance And Ranking Of Switchgrass Genotypes, M. D. Casler, S. Sosa, A. R. Boe, S. A. Bonos Jan 2019

Soil Quality And Region Influence Performance And Ranking Of Switchgrass Genotypes, M. D. Casler, S. Sosa, A. R. Boe, S. A. Bonos

Agronomy, Horticulture and Plant Science Faculty Publications

Development of switchgrass (Panicum virgatum L.) as a dedicated bioenergy feedstock requires intensive and extensive breeding programs that include careful and thoughtful consideration of appropriate target populations of environments (TPEs). The purpose of this study was to evaluate region (climate), soil quality, and N fertilization level as potential factors influencing the choice of TPE. A total of 45 switchgrass genotypes were evaluated in uniform field studies at six field sites defined as prime or marginal soils in New Jersey, South Dakota, and Wisconsin. Region and soil quality had strong interactions with genotype, but N fertilization had little impact on …


Mob Grazing Results In High Forage Utilization And Reduced Western Snowberry Size, Heidi Reed, Sharon Clay, Alexander Smart, David Clay, Michelle Ohrtman Jan 2019

Mob Grazing Results In High Forage Utilization And Reduced Western Snowberry Size, Heidi Reed, Sharon Clay, Alexander Smart, David Clay, Michelle Ohrtman

Agronomy, Horticulture and Plant Science Faculty Publications

Mob-grazing strives to maximize forage utilization and minimize selective grazing by using high stocking densities in small paddocks for short durations (12–24 hr). Rotational-grazing uses low stocking densities for a longer time period, retaining about half of the original available forage; although selective grazing can occur. Three cattle (Bos taurus × Bos indicus) grazing intensities: mob- (stocking densities from 32,000 to 67,000 kg ha−1; duration—24 hr); rotation (stocking density—2500 kg ha−1; duration—35 d); and non-grazed systems were compared based on forage utilization and changes to western snowberry (Symphoricarpos occidentalis) (WS) patch volume in a 2-year South Dakota study. …