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- Keyword
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- Amyotrophic lateral sclerosis; ALS; superoxide dimutase 1; Drosophila Melanogaster; protein aggregation; (1)
- Biology (1)
- Ssal; Hsp70; N-terminal acetylation; PTMs; protein purification; Hsp40; Molecular chaperones; Ydj1; Post-translational modification; chaperone modification; one-step purification; (1)
Articles 1 - 2 of 2
Full-Text Articles in Life Sciences
Detecting Aggregated Superoxide-Dismutase Protein Using Aggregate Specific Antibodies, Helen Magana
Detecting Aggregated Superoxide-Dismutase Protein Using Aggregate Specific Antibodies, Helen Magana
Honors Projects
Amyotrophic lateral sclerosis is neurodegenerative disease characterized by late-onset, loss of motor neurons, paralysis, and eventual death. SOD1 is a free radical scavenger that normally resides in the cytoplasm, nucleus, and intermembrane space of the mitochondria. Mutations in SOD1, a ubiquitously expressed free-radical scavenger, are one cause of ALS. Degeneration of motor neurons is thought to be triggered by aggregation of mutant SOD1 followed by propagation to adjacent cells though cell-cell contacts. Here, we present immunohistochemical findings on tissues of a Drosophila melanogaster expressing mutant dsod1 alleles at the endogenous locus. Aggregate-specific antibodies were used to assess the presence of …
The Purification And Characterization Of N-Terminally Acetylated Ssal From Saccharomyces Cerevisiae, Alijah A. Griffith
The Purification And Characterization Of N-Terminally Acetylated Ssal From Saccharomyces Cerevisiae, Alijah A. Griffith
Honors Projects
Molecular chaperones are highly conserved, ubiquitous enzymes that maintain proteostasis by mediating protein structure in vivo. Post-translational modifications (PTMs) can act as a functional switch between chaperone pathways, allowing for cells to enact a coordinated response to cellular perturbations. Several PTMs are well characterized in the context of chaperone function, though the role of N-terminal acetylation is commonly overlooked, leading to an overwhelming lack of scientific understanding in this area of study. Ssa1, a Hsp70 class chaperone endogenous to Saccharomyces cerevisiae, is likely N-terminally acetylated, though in vitro studies of Ssa1 are limited by current purification methods which are resource-intensive. …