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The Cγ Subunit Is A Unique Isozyme Of The Camp-Dependent Protein Kinase, Stephen J. Beebe, Paul Salomonsky, Tore Jahnsen, Yixin Li
The Cγ Subunit Is A Unique Isozyme Of The Camp-Dependent Protein Kinase, Stephen J. Beebe, Paul Salomonsky, Tore Jahnsen, Yixin Li
Bioelectrics Publications
There are at least three isozymes (Cα, Cβ, and Cγ) of the mammalian catalytic (C) subunit of cAMP-dependent protein kinase (PKA) (Beebe, S., Oyen, O., Sandberg, M., Froysa, A., Hansson, V., and Jahnsen, T. (1990) Mol. Endocrinol. 4, 465-475). To compare the Cγ and Cα isozymes, the respective cDNAs were expressed in permanently transformed Kin-8 PKA-deficient Y1 adrenal cells using the mouse metallothionein promoter. The recombinant C subunits were characterized as immunoreactive, zinc-inducible, cAMP-dependent kinase activities. In contrast to Cα, histone was a better substrate than Leu-Arg-Arg-Ala-Ser-Leu-Gly (Kemptide) for Cγ. Furthermore, Cγ histone kinase activity was not inhibited by the …
Molecular And Physiological Responses Of Diatoms To Variable Levels Of Irradiance And Nitrogen Availability: Growth Of Skeletonema Costatum In Simulated Upwelling Conditions, G. Jason Smith, Richard C. Zimmerman, Randall S. Alberte
Molecular And Physiological Responses Of Diatoms To Variable Levels Of Irradiance And Nitrogen Availability: Growth Of Skeletonema Costatum In Simulated Upwelling Conditions, G. Jason Smith, Richard C. Zimmerman, Randall S. Alberte
OES Faculty Publications
Molecular mechanisms that drive metabolic acclimation to environmental shifts have been poorly characterized in phytoplankton. In this laboratory study. the response of light- and N-limited Skeletonema costatum cells to an increase in light and NO3 availability was examined. C assimilation was depressed relative to N assimilation early in enrichment, and the photosynthetic quotient (O2: CO2) increased, consistent with the shunting of reducing equivalents from CO2 fixation to NO3- reduction. The concomitant increase in dark respiration was consistent with the increased energetic demand associated with macromolecular synthesis. The accelerations of N-specific rates of …