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Full-Text Articles in Life Sciences
Investigation Of Glycoside Hydrolases And Calcium In Cytophaga Hutchinsonii Cellulose Utilization, Choua Kou Vang
Investigation Of Glycoside Hydrolases And Calcium In Cytophaga Hutchinsonii Cellulose Utilization, Choua Kou Vang
All Graduate Theses, Dissertations, and Other Capstone Projects
Cytophaga hutchinsonii is a soil bacterium that can utilize cellulose as a carbon source. The cellulose utilization system of C. hutchinsonii has been predicted to be novel and its endoglucanases could potentially be used in the production of cellulosic based biofuels. These enzymes could be used to process cellulose to glucose or other soluble sugars, which can be further converted to ethanol by yeast fermentation. Current biofuels utilize starches, primarily from corn, which has direct competition with farming space for food crops. This study focuses on observing the growth of C. hutchinsonii knockout mutant strains on cellulose substrate to better …
Investigation Of Fermentation Efficiency In Saccharomyces Cerevisiae Through Telomere Integrity And Maltase Expression, Dana Bennett
Investigation Of Fermentation Efficiency In Saccharomyces Cerevisiae Through Telomere Integrity And Maltase Expression, Dana Bennett
All Graduate Theses, Dissertations, and Other Capstone Projects
Many microbreweries practice serial fermentation, or serial repitching. Serial repitching is the process by which one yeast culture is reused for multiple batches of beer. Each batch of subsequent beer is called a “pitch.” This technique helps breweries limit production costs. However, fermentation is difficult to predict throughout serial fermentation. This leads to beer quality issues such as fluctuation in alcohol production, buildup of unwanted flavor compounds, and decreased carbon dioxide production, which results in reduced profit. To combat this issue, many breweries will attempt to predict fermentation efficiency through viable cell counting in order to ensure a consistent number …
Nisin Resistance Of Bacillus Cerus: Preparation Of Nisin, Peter Weber
Nisin Resistance Of Bacillus Cerus: Preparation Of Nisin, Peter Weber
Journal of Undergraduate Research at Minnesota State University, Mankato
Nisin is a peptide that is made by the bacterium Lactococcus lactis. Nisin is a small molecule that kills gram positive bacteria by binding to their membrane and by disrupting the proton motive force. When food is processed it is heated to kill bacteria, but some bacteria still survive. Adding nisin to the food provides a second barrier for the growth of the bacteria. Purified nisin has become quite expensive in the current marketplace. Therefore this study is directed at producing nisin and purifying it. L. lactis was grown in five different media (BHI, BHI + 1% glucose, BHI + …
The Innate Immune Response In Eisenia Fetida To Microbial Challenges, Aaron Schindler
The Innate Immune Response In Eisenia Fetida To Microbial Challenges, Aaron Schindler
Journal of Undergraduate Research at Minnesota State University, Mankato
The common earthworm, Eisenia fetida, exhibits a rudimentary immune system. The earthworm needs cellular and chemical responses against a constant microbial exposure from its natural environment. Some cellular and chemical responses are found in the coelomic fluid and have been shown to demonstrate anti-microbial characteristics. This project uses microscopy and modified staining techniques to differentiate and categorize the cellular components found in the coelomic fluid. Following a microbial challenge by Klebsiella pneumoniae, an inflammatory response was initiated. Six groups of earthworms were injected with 0.05 ml of 1.0 x 106 cfu /ml K. pneumoniae on day one and tested over …