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Molecular Mechanisms Of Bacillus Thuringiensis Resistance In The Sugarcane Borer, Yunlong Yang
Molecular Mechanisms Of Bacillus Thuringiensis Resistance In The Sugarcane Borer, Yunlong Yang
LSU Doctoral Dissertations
The sugarcane borer, Diatraea saccharalis, is a major target pest of transgenic corn expressing Bacillus thuringiensis (Bt) proteins in many areas of the U.S. mid-southern region. A Cry1Ab-resistant (Cry1Ab-RR) strain of D. saccharalis has been developed from a single two-parent family-line. To examine the molecular mechanisms of the Cry1Ab resistance in this insect strain, cDNAs of five types of potential candidate genes related to Bt resistance were sequenced using reverse transcriptase polymerase chain reaction (RT-PCR) and 5’ rapid amplification of cDNA end (5’ RACE). The Bt resistance candidate genes examined included three trypsins (DsTRYs), three chymotrypsins (DsCHYs), three aminopeptidases N …
Ecology And Chemical Ecology Of Plant-Insect Interactions In Rice: Implications For Pest Management, Jason Charles Hamm
Ecology And Chemical Ecology Of Plant-Insect Interactions In Rice: Implications For Pest Management, Jason Charles Hamm
LSU Doctoral Dissertations
Induced resistance to the rice water weevil, Lissorhoptrus oryzophilus, was assessed in greenhouse and field experiments. The fall armyworm,Spodoptera frugiperda, and an elicitor, jasmonic acid, were used to induce resistance. The effect of these treatments on rice resistance to oviposition varied between cultivar used, but significantly fewer larvae were found on plants exposed to S. frugiperda and jasmonic acid on both cultivars tested. Application of jasmonic acid significantly reduced the number of L. oryzophilus larvae per plant, and represents the first example of elicitor-induced resistance in rice in field experiments. Oviposition by the sugarcane borer, Diatraea saccharalis, induced resistance to …
Molecular Mechanisms Of Bacillus Thuringiensis Resistance In The Sugarcane Borer, Yunlong Yang
Molecular Mechanisms Of Bacillus Thuringiensis Resistance In The Sugarcane Borer, Yunlong Yang
LSU Doctoral Dissertations
The sugarcane borer, Diatraea saccharalis, is a major target pest of transgenic corn expressing Bacillus thuringiensis (Bt) proteins in many areas of the U.S. mid-southern region. A Cry1Ab-resistant (Cry1Ab-RR) strain of D. saccharalis has been developed from a single two-parent family-line. To examine the molecular mechanisms of the Cry1Ab resistance in this insect strain, cDNAs of five types of potential candidate genes related to Bt resistance were sequenced using reverse transcriptase polymerase chain reaction (RT-PCR) and 5’ rapid amplification of cDNA end (5’ RACE). The Bt resistance candidate genes examined included three trypsins (DsTRYs), three chymotrypsins (DsCHYs), three aminopeptidases N …