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Examination Of Blueberry Anthocyanins In Prevention Of Age-Related Macular Degeneration Through Retinal Pigment Epithelial Cell Culture Study, Naomi Marie Sundalius
Examination Of Blueberry Anthocyanins In Prevention Of Age-Related Macular Degeneration Through Retinal Pigment Epithelial Cell Culture Study, Naomi Marie Sundalius
LSU Master's Theses
This research investigated the ability of blueberry anthocyanins to inhibit the uptake of N-retinyl-N-retinylidene ethanolamine (A2E) by retinal pigment epithelial (RPE) cells as quantified by pigment epithelial derived factor (PEDF) levels in RPE cells. The A2E was synthesized and identified by matrix-assisted laser desorption/ionization mass spectrometry (MALDI-MS). Freeze dried blueberry powder of 50/50 blend (Tifblue/Rubel) anthocyanins were extracted by acetone and concentrated through chromatography. A RPE cell culture study investigating four treatments using a combination of 100 ìM A2E in DMSO and 100 ìg of blueberry anthocyanins in DMSO was tested. The treatments included: (A) control RPE cells; (B) RPE …