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Full-Text Articles in Life Sciences
Effect Of Culture Conditions On Gene Expression In Manipulated Bovine Embryos, Megan Nicole Purpera
Effect Of Culture Conditions On Gene Expression In Manipulated Bovine Embryos, Megan Nicole Purpera
LSU Master's Theses
Numerous studies have reported aberrant gene expression levels attributed to suboptimal in vitro culture conditions presented to embryos. Since the culture environment is a common aspect of both in vitro production (IVP) and nuclear transfer (NT), research focusing on the in vitro culture system will have the potential to improve both techniques. This study investigated the effects of different culture systems and protein sources on the developmental competence of IVP embryos measured by cleavage and blastocyst rates, cell number, and relative abundance of oct-4, nanog, connexin 43, and GLUT-1 transcripts when compared to in vivo embryos. Experiment 1 compared IVP …
Effects Of Cryopreservation And Constituents Of Semen Extenders On Mitochondrial Function Of Bull Spermatozoa, Abdulhakeem Hashim Eljarah
Effects Of Cryopreservation And Constituents Of Semen Extenders On Mitochondrial Function Of Bull Spermatozoa, Abdulhakeem Hashim Eljarah
LSU Doctoral Dissertations
This study investigated the effects of semen extender constituents and cryopreservation on bovine spermatozoal mitochondrial function. Three yearling Holstein bulls were used. Two ejaculates per bull were collected and pooled on a weekly basis for five weeks and extended in four treatments: 1) sodium citrate egg yolk extender with antibiotics (lincomycin, spectinomycin, gentamicin and tylosin); 2) ¡°1¡± with glycerol; 3) ¡°2¡± without antibiotics; and 4) ¡°1¡± without antibiotics. Each was divided into portions for analyses before freezing and after cryopreservation. The pre-freeze and thawed semen were transferred to a 37¡ãC water bath, the same assays were performed. In experiment 1, …
In Utero And In Vitro Sex Ratio Of Bovine Embryos And Calves Originating From The Left And Right Ovaries, Darin Alan Hylan
In Utero And In Vitro Sex Ratio Of Bovine Embryos And Calves Originating From The Left And Right Ovaries, Darin Alan Hylan
LSU Doctoral Dissertations
An asymmetric distribution of the sexes within the left and right uterine horns has been described in multiple polytocous, laboratory species. A series of experiments were conducted to evaluate the sex ratio (% male) of calves gestated in the left and right uterine horns, as well as the sex ratio of embryos originating from the left and right ovaries of cattle. In Experiment 1, the sex ratio of calves and fetuses gestated in the left and right uterine horns was investigated. The sex ratio of calves and fetuses gestated in the right uterine horn was significantly higher compared with the …