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Inhibition Of Hormone Dependent And Independent Breast Cancer Cells By Oyster(Crassostrea Virginica) Ceramide, Madhavi Chintalapati
Inhibition Of Hormone Dependent And Independent Breast Cancer Cells By Oyster(Crassostrea Virginica) Ceramide, Madhavi Chintalapati
LSU Master's Theses
The purpose of this research was to investigate the in vitro and in vivo anti-angiogenic properties of ceramide isolated from oyster (Crassostrea virginica). Ceramide was isolated from oyster by silicic acid chromatography, purified by alkaline hydrolysis, and analyzed by electrospray ionization mass spectrometry (ESI-MS). In vitro, the activity of ceramide on human hormone-dependent (MCF-7) and hormone-independent (MDA-MB-435s) breast cancer cells was evaluated for cell viability and proliferation, VEGF-induced cell migration and invasion, apoptosis, and autophagy. The activity of ceramide was also evaluated for VEGF-induced human umbilical vein endothelial cells (HUVEC) tube formation. The protein levels of vascular endothelial growth factor …