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Determining Gene Copy Number In Transfected Caprine Fibroblast Cells, Jessica A. Wilson
Determining Gene Copy Number In Transfected Caprine Fibroblast Cells, Jessica A. Wilson
LSU Master's Theses
Transgene expression in stably transgenic organisms is affected by many factors, including the copy number of the transgene in the genome, and by interactions between the transgene and flanking DNA sequences. Transgene copy number has also been shown to effect genetic stability in transgenic plants. Two commonly used methods for transfecting cells are liposome mediated transfection and electroporation. Little is known about the mean transgene copy number or variability of the copy number with these techniques. Quantitative PCR (Q-PCR) has been shown to be an effective method for determining transgene copy number. The objective of this study was to determine …