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Estrogen Content Of Dairy And Swine Wastes, D. Raj Raman, Elizabeth L. Williams, Alice C. Layton, Robert T. Burns, James P. Easter, Adam S. Daugherty, Michael D. Mullen, Gary S. Sayler
Estrogen Content Of Dairy And Swine Wastes, D. Raj Raman, Elizabeth L. Williams, Alice C. Layton, Robert T. Burns, James P. Easter, Adam S. Daugherty, Michael D. Mullen, Gary S. Sayler
D. Raj Raman
Naturally occurring estrogens in animal wastes may cause negative environmental impacts, yet their abundance in animal waste treatment and storage structures is poorly documented. To better quantify estrogen concentra tions in animal wastes, multiple waste samples were collected from treatment and storage structures at dairy and swine facilities and analyzed for concentrations of 17β-estradiol (E2), estrone (E1), and 17α-estradiol by gas chromatography−mass spectroscopy and by enzyme linked immunosorbent assay (E2 only). Mass ratios of each estrogen to the macronutrients nitrogen, phosphorus, and potassium were also determined. Because manure application rates are typically macronutrient-based, estrogen to macronutrient ratios are proportional to …